{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T04:59:33Z","timestamp":1787979573451,"version":"build-2784847793"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,4,18]],"date-time":"2023-04-18T00:00:00Z","timestamp":1681776000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Chimeric antigen receptor (CAR) T cells targeted to the CD19 B\u2010cell antigen form an approved treatment for patients with relapsed\/refractory diffuse large B\u2010cell lymphoma (r\/r DLBCL). However, since this therapy is administered after multiple lines of treatment and exposure to lymphotoxic agents, there is an urgent need to optimize this modality of treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>To circumvent the difficulties of harvesting adequate and optimal T cells from DLBCL patients and improve CART therapy, we suggest an earlier lymphopheresis (i.e. at first relapse, before salvage treatment). We conducted a prospective study and evaluated the potential benefit of an earlier lymphopheresis (early group, <jats:italic>n<\/jats:italic>\u2009=\u200922) on the clinical outcome of CD19\u2010CART infused DLBCL patients, in comparison with standard lymphopheresis (i.e. at second relapse and beyond; standard group, <jats:italic>n<\/jats:italic>\u2009=\u200923).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>An increased percentage of na\u00efve T cells and increased in vitro T\u2010cell functionality were observed in the early group. Additionally, these cells exhibit a lower exhaustion profile than T cells collected in the standard group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>While improved T\u2010cell phenotype and function in the lymphopheresis product did not translate into significantly improved clinical outcomes, a trend towards better overall survival (OS) and progression\u2010free survival (PFS) was observed. Early lymphopheresis maximizes the potential of salvage therapies, without compromising CAR T\u2010cell quality.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bjh.18816","type":"journal-article","created":{"date-parts":[[2023,4,18]],"date-time":"2023-04-18T08:35:57Z","timestamp":1681806957000},"page":"74-85","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Early lymphocyte collection for <scp>anti\u2010CD19 CART<\/scp> production improves T\u2010cell fitness in patients with relapsed\/refractory diffuse large B\u2010cell lymphoma"],"prefix":"10.1111","volume":"202","author":[{"given":"T.","family":"Dubnikov\u00a0Sharon","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Department of Bone Marrow Transplantation and Cancer Immunotherapy, Hadassah Medical Center Hebrew University of Jerusalem  Jerusalem Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Assayag","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Bone Marrow Transplantation and Cancer Immunotherapy, Hadassah Medical Center Hebrew University of Jerusalem  Jerusalem Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8516-2751","authenticated-orcid":false,"given":"B.","family":"Avni","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Bone Marrow Transplantation and Cancer Immunotherapy, Hadassah Medical Center Hebrew University of Jerusalem  Jerusalem Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Kfir\u2010Erenfeld","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Bone Marrow Transplantation and Cancer Immunotherapy, Hadassah Medical Center Hebrew University of Jerusalem  Jerusalem Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Lebel","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Hematology, Hadassah Medical Center Hebrew University of Jerusalem  Jerusalem Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9426-4482","authenticated-orcid":false,"given":"M. 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