{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T19:41:21Z","timestamp":1779392481162,"version":"3.53.1"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,5,3]],"date-time":"2023-05-03T00:00:00Z","timestamp":1683072000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970188"],"award-info":[{"award-number":["81970188"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>In this prospective study, we aimed to evaluate the utility of circulating tumour DNA (ctDNA) in peripheral T\u2010cell lymphomas (PTCLs). Plasma cell\u2010free DNA (cfDNA) was obtained, and the mutational profile was assessed in 47 patients with newly diagnosed mature T\u2010 and NK\u2010cell lymphoma. To validate the mutations detected in cfDNA, paired tumour tissue samples were available for 36 patients. Targeted next\u2010generation sequencing was performed. A total of 279 somatic mutations involving 149 genes were identified in the 47 cfDNA samples. The overall sensitivity of plasma cfDNA in discovering biopsy\u2010confirmed mutations was 73.9% with a specificity of 99.6%. When we considered only mutations with variant allele frequency &gt;5% in the tumour biopsy, the sensitivity increased to 81.9%. Pretreatment ctDNA concentration and the number of mutations were highly correlated with tumour burden indicators including lactate dehydrogenase, Ann Arbor stage and International Prognostic Index score. Patients with high ctDNA level (&gt;1.9 log ng\/mL) had significantly lower overall response rates, inferior 1\u2010year progression\u2010free survival and overall survival rates than those with low level. Longitudinal analysis of ctDNA showed a strong agreement between ctDNA dynamics and the radiographic response. In conclusion, our study demonstrates that ctDNA may serve as a promising tool for mutational profiling, tumour burden assessment, outcome prediction and disease monitoring in PTCLs.<\/jats:p>","DOI":"10.1111\/bjh.18824","type":"journal-article","created":{"date-parts":[[2023,5,3]],"date-time":"2023-05-03T08:23:35Z","timestamp":1683102215000},"page":"86-95","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Mutation profiling, tumour burden assessment, outcome prediction and disease monitoring by circulating tumour <scp>DNA<\/scp> in peripheral T\u2010cell lymphoma"],"prefix":"10.1111","volume":"202","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9690-8555","authenticated-orcid":false,"given":"Chong","family":"Wei","sequence":"first","affiliation":[{"name":"Department of Hematology, Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing 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