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We aimed to reveal a unique amino acid sequence (AA) of complementarity\u2010determining region 3 (CDR3) of the T\u2010cell receptor (TCR)\u03b2 and TCR\u03b1 chains of HLA\u2010A*02:01\u2010restricted Tax<jats:sub>11\u201319<\/jats:sub>\u2010specific cytotoxic T cells (Tax\u2010CTLs). The gene expression profiles (GEP) of Tax\u2010CTLs were assessed by the next\u2010generation sequence (NGS) method with SMARTer technology. Tax\u2010CTLs seemed to be oligoclonal, and their gene compositions were skewed. The unique motifs of \u2018DSWGK\u2019 in TCR\u03b1 and \u2018LAG\u2019 in TCR\u03b2 at CDR3 were observed in almost all patients. Tax\u2010CTL clones harbouring the \u2018LAG\u2019 motif with BV28 had a higher binding score than those without either of them, besides a higher binding score associated with longer survival. Tax\u2010CTLs established from a single cell showed killing activities against Tax\u2010peptide\u2010pulsed HLA\u2010A2<jats:sup>+<\/jats:sup>T2 cell lines. GEP of Tax\u2010CTLs revealed that genes associated with immune response activity were well preserved in long\u2010term survivors with stable status. These methods and results can help us better understand immunity against ATL, and should contribute to future studies on the clinical application of adoptive T\u2010cell therapies.<\/jats:p>","DOI":"10.1111\/bjh.18918","type":"journal-article","created":{"date-parts":[[2023,6,15]],"date-time":"2023-06-15T08:19:06Z","timestamp":1686817146000},"page":"578-588","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Gene expression and<scp>TCR<\/scp>amino acid sequences selected by<scp>HLA\u2010A02<\/scp>:01\u2010restricted<scp>CTLs<\/scp>specific to<scp>HTLV<\/scp>\u20101 in<scp>ATL<\/scp>patients"],"prefix":"10.1111","volume":"202","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3313-860X","authenticated-orcid":false,"given":"Machiko","family":"Kusuda","sequence":"first","affiliation":[{"name":"Division of Hematology Jichi Medical University Saitama Medical Center Saitama 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