{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T04:58:22Z","timestamp":1787979502763,"version":"build-2784847793"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,8,31]],"date-time":"2023-08-31T00:00:00Z","timestamp":1693440000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>To determine the prevalence and clinical relevance of glycosylphosphatidylinositol\u2010anchored protein\u2010deficient (GPI[\u2212]) cell populations (paroxysmal nocturnal haemoglobinuria [PNH]\u2010type cells) in patients with acquired aplastic anaemia (AA) or myelodysplastic syndrome (MDS), we prospectively studied peripheral blood samples of 2402 patients (1075 with AA, 900 with MDS, 144 with PNH, and 283 with other anaemia) using a high\u2010sensitivity flow cytometry assay in a nationwide multi\u2010centre observational study. PNH\u2010type cells were detected in 52.6% of AA and 13.7% of MDS patients. None of the 35 patients with refractory anaemia (RA) with ringed sideroblasts or the 86 patients with RA with excess of blasts carried PNH\u2010type cells. Among the 317 patients possessing PNH\u2010type granulocytes, the percentage of PNH\u2010type granulocytes increased by \u226510% in 47 patients (14.8%), remained unchanged in 240 patients (75.7%), and decreased by \u226510% in 30 patients (9.5%) during 3\u2009years of follow\u2010up. PNH\u2010type granulocyte expansion occurred more frequently (27.1%) in the 144 patients who originally carried PNH\u2010type granulocytes \u22651% than in the 173 patients with PNH\u2010type granulocytes &lt;1% (4.6%). This study confirmed that PNH\u2010type cells are undetectable in authentic clonal MDS patients, and the presence of \u22651% PNH\u2010type granulocytes predicts a higher likelihood of PNH\u2010type cell expansion than with &lt;1% PNH\u2010type granulocytes.<\/jats:p>","DOI":"10.1111\/bjh.19048","type":"journal-article","created":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T06:31:15Z","timestamp":1693549875000},"page":"468-476","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A longitudinal analysis of paroxysmal nocturnal haemoglobinuria\u2010type cells in patients with bone marrow failure: Results of a prospective multi\u2010centre study in Japan"],"prefix":"10.1111","volume":"203","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6189-0620","authenticated-orcid":false,"given":"Ken","family":"Ishiyama","sequence":"first","affiliation":[{"name":"Department of Hematology, Faculty of Medicine Institute of Medical Pharmaceutical and Health Sciences, Kanazawa University  Kanazawa Japan"},{"name":"Department of Hematology National Center for Global Health and Medicine  Tokyo Japan"},{"name":"Japan PNH Study Group  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuji","family":"Yonemura","sequence":"additional","affiliation":[{"name":"Japan PNH Study Group  Tokyo Japan"},{"name":"Department of Transfusion Medicine and Cell Therapy Kumamoto University  Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuya","family":"Kawaguchi","sequence":"additional","affiliation":[{"name":"Japan PNH Study Group  Tokyo Japan"},{"name":"Department of Medical Technology Kumamoto Health Science University  Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3423-1523","authenticated-orcid":false,"given":"Kohei","family":"Hosokawa","sequence":"additional","affiliation":[{"name":"Department of Hematology, Faculty of Medicine Institute of Medical Pharmaceutical and Health Sciences, Kanazawa University  Kanazawa Japan"},{"name":"Japan PNH Study Group  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiharu","family":"Sugimori","sequence":"additional","affiliation":[{"name":"Japan PNH Study Group  Tokyo Japan"},{"name":"Department of Hematology Ishikawa Prefectural Central Hospital  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasutaka","family":"Ueda","sequence":"additional","affiliation":[{"name":"Japan PNH Study Group  Tokyo Japan"},{"name":"Department of Hematology and Oncology Osaka University Graduate School of Medicine  Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Takamori","sequence":"additional","affiliation":[{"name":"Japan PNH Study Group  Tokyo Japan"},{"name":"Department of Hematology and Oncology Osaka University Graduate School of Medicine  Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6563-3093","authenticated-orcid":false,"given":"Naoshi","family":"Obara","sequence":"additional","affiliation":[{"name":"Japan PNH Study Group  Tokyo Japan"},{"name":"Department of Medical Sciences, Hematology Tsukuba University  Tsukuba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideyoshi","family":"Noji","sequence":"additional","affiliation":[{"name":"Japan PNH Study Group  Tokyo Japan"},{"name":"Department of Hematology Fukushima Medical University  Fukushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukari","family":"Shirasugi","sequence":"additional","affiliation":[{"name":"Japan PNH Study Group  Tokyo Japan"},{"name":"Department of Hematology and Oncology Tokai University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiyoshi","family":"Ando","sequence":"additional","affiliation":[{"name":"Japan PNH Study Group  Tokyo Japan"},{"name":"Department of Hematology and Oncology Tokai University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsutomu","family":"Shichishima","sequence":"additional","affiliation":[{"name":"Japan PNH Study Group  Tokyo Japan"},{"name":"Department of Hematology Fukushima Medical University  Fukushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haruhiko","family":"Ninomiya","sequence":"additional","affiliation":[{"name":"Japan PNH Study Group  Tokyo Japan"},{"name":"Department of Hematology Tsukuba University  Tsukuba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7803-7338","authenticated-orcid":false,"given":"Shigeru","family":"Chiba","sequence":"additional","affiliation":[{"name":"Japan PNH Study Group  Tokyo Japan"},{"name":"Department of Hematology Tsukuba University  Tsukuba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5026-693X","authenticated-orcid":false,"given":"Jun\u2010ichi","family":"Nishimura","sequence":"additional","affiliation":[{"name":"Japan PNH Study Group  Tokyo Japan"},{"name":"Department of Hematology and Oncology Osaka University Graduate School of Medicine  Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuzuru","family":"Kanakura","sequence":"additional","affiliation":[{"name":"Japan PNH Study Group  Tokyo Japan"},{"name":"Department of Hematology and Oncology Osaka University Graduate School of Medicine  Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9674-624X","authenticated-orcid":false,"given":"Shinji","family":"Nakao","sequence":"additional","affiliation":[{"name":"Department of Hematology, Faculty of Medicine Institute of Medical Pharmaceutical and Health Sciences, Kanazawa University  Kanazawa Japan"},{"name":"Japan PNH Study Group  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,31]]},"reference":[{"issue":"1","key":"e_1_2_10_2_1","first-page":"81","article-title":"A pathogenetic link between aplastic anemia and paroxysmal nocturnal hemoglobinuria is suggested by a high frequency of aplastic anemia patients with a deficiency of phosphatidylinositol glycan anchored proteins","volume":"23","author":"Schrezenmeier H","year":"1995","journal-title":"Exp Hematol"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003\u20104819\u2010131\u20106\u2010199909210\u201000002"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102002\u201003\u20100799"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102141.2009.07822.x"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.017889"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.12661"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21139"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21480"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1532\/IJH97.06077"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375\u2010021\u201001190\u20109"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102005\u201006\u20102485"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277\u2010015\u20102348\u20105"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2015.132530"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/114.3.459"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277\u2010018\u20103443\u20101"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0054046"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0140\u20106736(88)90388\u20101"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102005\u201010\u20104149"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.13601"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06240.x"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21610"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102002\u201012\u20103706"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1414799"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100609.2011.01615.x"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.15443"}],"container-title":["British Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bjh.19048","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T10:58:05Z","timestamp":1698058685000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bjh.19048"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,31]]},"references-count":25,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,11]]}},"alternative-id":["10.1111\/bjh.19048"],"URL":"https:\/\/doi.org\/10.1111\/bjh.19048","archive":["Portico"],"relation":{},"ISSN":["0007-1048","1365-2141"],"issn-type":[{"value":"0007-1048","type":"print"},{"value":"1365-2141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,31]]},"assertion":[{"value":"2023-04-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}