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In this study, we found that the copy number of prune exopolyphosphatase 1 (<jats:italic>PRUNE1<\/jats:italic>), located on chromosome 1q21.3, could further stratify the prognosis of MM patients with 1q21+. Using selected reaction monitoring\/multiple reaction monitoring (SRM\/MRM) analysis, liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS), transmission electron microscopy (TEM), confocal fluorescence microscopy, calculation of adenosine triphosphate (ATP), intracellular reactive oxygen species (ROS) and mitochondrial oxygen consumption rates (OCRs), we demonstrated for the first time that PRUNE1 promotes the proliferation and invasion of MM cells by stimulating purine metabolism, purine synthesis enzymes and mitochondrial functions, enhancing links between purinosomes and mitochondria. SOX11 was identified as a transcription factor for PRUNE1. Through integrated analysis of the transcriptome and proteome, CD73 was determined to be the downstream target of PRUNE1. Furthermore, it has been determined that dipyridamole can effectively suppress the proliferation of MM cells with high\u2010expression levels of PRUNE1 in vitro and in vivo. These findings provide insights into disease\u2010causing mechanisms and new therapeutic targets for MM patients with 1q21+.<\/jats:p>","DOI":"10.1111\/bjh.19088","type":"journal-article","created":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T01:48:25Z","timestamp":1693878505000},"page":"599-613","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<scp>PRUNE1<\/scp> (located on chromosome 1q21.3) promotes multiple myeloma with 1q21 Gain by enhancing the links between purine and mitochondrion"],"prefix":"10.1111","volume":"203","author":[{"given":"Jiadai","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Hematology Zhongshan Hospital, Fudan University  Shanghai China"},{"name":"Cancer Center Zhongshan Hospital, Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yawen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of 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