{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:57:59Z","timestamp":1787317079160,"version":"build-2736575974"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T00:00:00Z","timestamp":1694476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005010","name":"Associazione Italiana per la Ricerca sul Cancro","doi-asserted-by":"publisher","award":["21198"],"award-info":[{"award-number":["21198"]}],"id":[{"id":"10.13039\/501100005010","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>Blinatumomab is the first bi\u2010specific T\u2010cell engager approved for relapsed or refractory B\u2010cell precursor acute lymphoblastic leukaemia (B\u2010ALL). Despite remarkable clinical results, the effects of blinatumomab on the host immune cell repertoire are not fully elucidated. In the present study, we characterized the peripheral blood (PB) and, for the first time, the bone marrow (BM) immune cell repertoire upon blinatumomab treatment. Twenty\u2010nine patients with B\u2010ALL received blinatumomab according to clinical practice. Deep multiparametric flow cytometry was used to characterize lymphoid subsets during the first treatment cycle. Blinatumomab induced a transient redistribution of PB effector T\u2010cell subsets and Treg cells with a persistent increase in cytotoxic NK cells, which was associated with a transient upregulation of immune checkpoint receptors on PB CD4 and CD8 T\u2010cell subpopulations and of CD39 expression on suppressive Treg cells. Of note, BM immune T\u2010cell subsets showed a broader post\u2010treatment subversion, including the modulation of markers associated with a T\u2010cell\u2010exhausted phenotype. In conclusion, our study indicates that blinatumomab differentially modulates the PB and BM immune cell repertoire, which may have relevant clinical implications in the therapeutic setting.<\/jats:p>","DOI":"10.1111\/bjh.19104","type":"journal-article","created":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T01:31:05Z","timestamp":1694568665000},"page":"637-650","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Blinatumomab differentially modulates peripheral blood and bone marrow immune cell repertoire: A Campus ALL study"],"prefix":"10.1111","volume":"203","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6976-4450","authenticated-orcid":false,"given":"Darina","family":"Ocadlikova","sequence":"first","affiliation":[{"name":"Dipartimento di Medicina Specialistica, Diagnostica e Sperimentale Universit\u00e0 di Bologna  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6510-8616","authenticated-orcid":false,"given":"Federico","family":"Lussana","sequence":"additional","affiliation":[{"name":"Department of Oncology and Hematology Universit\u00e0 degli Studi di Milano  Milan Italy"},{"name":"Hematology and Bone Marrow Transplant Unit, ASST Papa Giovanni XXIII  Bergamo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"Fracchiolla","sequence":"additional","affiliation":[{"name":"Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, UO Ematologia  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0716-1686","authenticated-orcid":false,"given":"Massimiliano","family":"Bonifacio","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina UOC Ematologia, Universit\u00e0 di Verona and AOUI Verona  Verona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lidia","family":"Santoro","sequence":"additional","affiliation":[{"name":"AO \u201cS. 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