{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:43:57Z","timestamp":1792183437158,"version":"4.4.0"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T00:00:00Z","timestamp":1702512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T00:00:00Z","timestamp":1702512000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100021839","name":"Snowdome Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100021839","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Flow cytometry (FC) incorporating the T\u2010cell receptor \u03b2 constant chain\u20101 (TRBC1) has been recently proposed as a new standard in T\u2010cell clonality assessment. While early studies demonstrated high sensitivity in samples with conspicuous tumour burden, performance in real\u2010world samples, including those with low tumour burden and correlation with molecular methods has been limited. We evaluated TRBC1\u2010FC performance and correlated the results with high\u2010throughput\n                    <jats:italic>TRB<\/jats:italic>\n                    sequencing and a targeted next\u2010generation sequencing gene panel. Our cohort consisted of 90 evaluable samples from 57 patients. TRBC1\u2010FC confirmed T\u2010cell clonality in 37 out of 38 samples (97%) that were involved in a mature T\u2010cell neoplasm (MTCN). T\u2010cell clonality was also identified in nine samples from patients lacking a current or prior diagnosis of MTCN, consistent with the emerging entity T\u2010cell clonality of uncertain significance. TRBC\u2010FC was polyclonal in all samples and negative for disease involvement by standard pathology assessment. However, correlation with\n                    <jats:italic>TRB<\/jats:italic>\n                    sequencing in 17 of these samples identified two cases that harboured the known clonal sequence from index testing, indicating the presence of measurable residual disease not otherwise detected. Our study provides real\u2010world correlative validation of TRBC1\u2010FC, highlighting the strengths and limitations pertinent to its increasing implementation by general diagnostic laboratories.\n                  <\/jats:p>","DOI":"10.1111\/bjh.19252","type":"journal-article","created":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T00:48:22Z","timestamp":1702601302000},"page":"910-920","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Evaluation of T\u2010cell clonality by\n                    <scp>anti\u2010TRBC1<\/scp>\n                    antibody\u2010based flow cytometry and correlation with T\u2010cell receptor sequencing"],"prefix":"10.1111","volume":"204","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4482-1014","authenticated-orcid":false,"given":"Phillip C.","family":"Nguyen","sequence":"first","affiliation":[{"name":"Department of Pathology Peter MacCallum Cancer Centre  Melbourne Victoria 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