{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:21:52Z","timestamp":1790706112999,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T00:00:00Z","timestamp":1716940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005564","name":"Gilead Sciences","doi-asserted-by":"publisher","award":["GLD18\/00063"],"award-info":[{"award-number":["GLD18\/00063"]}],"id":[{"id":"10.13039\/100005564","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009093","name":"Fundaci\u00f3n Espa\u00f1ola de Hematolog\u00eda y Hemoterapia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009093","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Circulating tumour DNA (ctDNA) allows genotyping and minimal residual disease (MRD) detection in lymphomas. Using a next\u2010generation sequencing (NGS) approach (EuroClonality\u2010NDC), we evaluated the clinical and prognostic value of ctDNA in a series of R\u2010CHOP\u2010treated diffuse large B\u2010cell lymphoma (DLBCL) patients at baseline (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200968) and after two cycles (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200959), monitored by metabolic imaging (positron emission tomography combined with computed tomography [PET\/CT]). A molecular marker was identified in 61\/68 (90%) ctDNA samples at diagnosis. Pretreatment high ctDNA levels significantly correlated with elevated lactate dehydrogenase, advanced stage, high\u2010risk International Prognostic Index and a trend to shorter 2\u2010year progression\u2010free survival (PFS). Valuable NGS data after two cycles of treatment were obtained in 44 cases, and 38 achieved major molecular response (MMR; 2.5\u2010log drop in ctDNA). PFS curves displayed statistically significant differences among those achieving MMR versus those not achieving MMR (2\u2010year PFS of 76% vs. 0%,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). Similarly, more than 66% reduction in \u0394SUVmax by PET\/CT identified two subgroups with different prognosis (2\u2010year PFS of 83% vs. 38%;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). Combining both approaches MMR and \u0394SUVmax reduction, a better stratification was observed (2\u2010year PFS of 84% vs. 17% vs. 0%,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). EuroClonality\u2010NDC panel allows the detection of a molecular marker in the ctDNA in 90% of DLBCL. ctDNA reduction at two cycles and its combination with interim PET results improve patient prognosis stratification.\n                  <\/jats:p>","DOI":"10.1111\/bjh.19458","type":"journal-article","created":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T22:45:06Z","timestamp":1717022706000},"page":"109-121","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Liquid biopsy for molecular characterization of diffuse large B\u2010cell lymphoma and early assessment of minimal residual disease"],"prefix":"10.1111","volume":"205","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3819-4846","authenticated-orcid":false,"given":"Miguel","family":"Alcoceba","sequence":"first","affiliation":[{"name":"Servicio de Hematologia, Hospital Universitario de Salamanca (HUS\/IBSAL) CIBERONC, y Centro de Investigacion del Cancer de Salamanca\u2010IBMCC (USAL\u2010CSIC)  Salamanca Spain"},{"name":"Grupo de trabajo cooperativo de linfomas y procesos linfoproliferativos de la SCLHH  Castilla y Le\u00f3n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James P.","family":"Stewart","sequence":"additional","affiliation":[{"name":"Patrick G Johnston Centre for Cancer Research Queens University Belfast  Belfast UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8864-1168","authenticated-orcid":false,"given":"Mar\u00eda","family":"Garc\u00eda\u2010\u00c1lvarez","sequence":"additional","affiliation":[{"name":"Servicio de Hematologia, Hospital Universitario de Salamanca (HUS\/IBSAL) CIBERONC, y Centro de Investigacion del Cancer de Salamanca\u2010IBMCC (USAL\u2010CSIC)  Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis G.","family":"D\u00edaz","sequence":"additional","affiliation":[{"name":"Servicio de Medicina Nuclear Hospital Universitario de Salamanca (HUS\/IBSAL)  Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5543-5154","authenticated-orcid":false,"given":"Cristina","family":"Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Servicio de Hematologia, Hospital Universitario de Salamanca (HUS\/IBSAL) CIBERONC, y Centro de Investigacion del Cancer de Salamanca\u2010IBMCC (USAL\u2010CSIC)  Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5609-2969","authenticated-orcid":false,"given":"Alejandro","family":"Medina","sequence":"additional","affiliation":[{"name":"Servicio de Hematologia, Hospital Universitario de Salamanca (HUS\/IBSAL) CIBERONC, y Centro de Investigacion del Cancer de Salamanca\u2010IBMCC (USAL\u2010CSIC)  Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1624-6059","authenticated-orcid":false,"given":"M. Carmen","family":"Chill\u00f3n","sequence":"additional","affiliation":[{"name":"Servicio de Hematologia, Hospital Universitario de Salamanca (HUS\/IBSAL) CIBERONC, y Centro de Investigacion del Cancer de Salamanca\u2010IBMCC (USAL\u2010CSIC)  Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Gazdova","sequence":"additional","affiliation":[{"name":"Patrick G Johnston Centre for Cancer Research Queens University Belfast  Belfast UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2667-0489","authenticated-orcid":false,"given":"Oscar","family":"Blanco","sequence":"additional","affiliation":[{"name":"Servicio de Anatom\u00eda Patol\u00f3gica Hospital Universitario de Salamanca (HUS\/IBSAL)  Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco J.","family":"D\u00edaz","sequence":"additional","affiliation":[{"name":"Servicio de Hematologia, Complejo Asistencial de Burgos  Burgos Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda J.","family":"Pe\u00f1arrubia","sequence":"additional","affiliation":[{"name":"Servicio de Hematologia, Hospital Cl\u00ednico Universitario de Valladolid  Valladolid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Servicio de Hematologia, Complejo Asistencial Universitario de Le\u00f3n  Le\u00f3n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Montes","sequence":"additional","affiliation":[{"name":"Servicio de Radiof\u00edsica y Protecci\u00f3n Radiol\u00f3gica Hospital Universitario de Salamanca (HUS\/IBSAL)  Salamanca 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y procesos linfoproliferativos de la SCLHH  Castilla y Le\u00f3n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Patrick G Johnston Centre for Cancer Research Queens University Belfast  Belfast UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7466-514X","authenticated-orcid":false,"given":"Pilar","family":"Tamayo","sequence":"additional","affiliation":[{"name":"Servicio de Medicina Nuclear Hospital Universitario de Salamanca (HUS\/IBSAL)  Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5834-9510","authenticated-orcid":false,"given":"Norma C.","family":"Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"Servicio de Hematologia, Hospital Universitario de Salamanca (HUS\/IBSAL) CIBERONC, y Centro de Investigacion del Cancer de Salamanca\u2010IBMCC 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