{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T16:01:07Z","timestamp":1768838467148,"version":"3.49.0"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,4,15]],"date-time":"2024-04-15T00:00:00Z","timestamp":1713139200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["14411950600"],"award-info":[{"award-number":["14411950600"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Juvenile myelomonocytic leukaemia (JMML) is a rare myeloproliferative neoplasm requiring haematopoietic stem cell transplantation (HSCT) for potential cure. Relapse poses a significant obstacle to JMML HSCT treatment, as the lack of effective minimal residual disease (MRD)\u2010monitoring methods leads to delayed interventions. This retrospective study utilized the droplet digital PCR (ddPCR) technique, a highly sensitive nucleic acid detection and quantification technique, to monitor MRD in 32 JMML patients. The results demonstrated that ddPCR detected relapse manifestations earlier than traditional methods and uncovered molecular insights into JMML MRD dynamics. The findings emphasized a critical 1\u2010 to 3\u2010month window post\u2010HSCT for detecting molecular relapse, with 66.7% (8\/12) of relapses occurring within this period. Slow MRD clearance post\u2010HSCT was observed, as 65% (13\/20) of non\u2010relapse patients took over 6\u2009months to achieve ddPCR\u2010MRD negativity. Furthermore, bone marrow ddPCR\u2010MRD levels at 1\u2010month post\u2010HSCT proved to be prognostically significant. Relapsed patients exhibited significantly elevated ddPCR\u2010MRD levels at this time point (<jats:italic>p<\/jats:italic>\u2009=\u20090.026), with a cut\u2010off of 0.465% effectively stratifying overall survival (<jats:italic>p<\/jats:italic>\u2009=\u20090.007), event\u2010free survival (<jats:italic>p<\/jats:italic>\u2009=\u20090.035) and cumulative incidence of relapse (<jats:italic>p<\/jats:italic>\u2009=\u20090.035). In conclusion, this study underscored ddPCR's superiority in JMML MRD monitoring post\u2010HSCT. It provided valuable insights into JMML MRD dynamics, offering guidance for the effective management of JMML.<\/jats:p>","DOI":"10.1111\/bjh.19465","type":"journal-article","created":{"date-parts":[[2024,4,16]],"date-time":"2024-04-16T02:04:54Z","timestamp":1713233094000},"page":"2332-2341","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Droplet digital <scp>PCR<\/scp>: An effective method for monitoring and prognostic evaluation of minimal residual disease in <scp>JMML<\/scp>"],"prefix":"10.1111","volume":"204","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-0893-0733","authenticated-orcid":false,"given":"Shengqiao","family":"Mao","sequence":"first","affiliation":[{"name":"Department of Hematology and Oncology Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuchen","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0638-5397","authenticated-orcid":false,"given":"Xia","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Miao","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changying","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengjuan","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianmin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohang","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junchen","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2895-700X","authenticated-orcid":false,"given":"Jing","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4,15]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-022-01613-1"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-11-844688"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-08-550483"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41409-023-01942-4"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2019.11.029"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-05-1944"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-03-491589"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.14778"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.17098"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2005.05489.x"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.24259"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-023-05448-3"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2016-0900"},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41540-023-00303-7"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3400"},{"key":"e_1_2_14_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics10030143"},{"key":"e_1_2_14_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac202028g"},{"key":"e_1_2_14_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-09-601690"},{"key":"e_1_2_14_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-020-0817-x"},{"key":"e_1_2_14_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-02217-x"},{"key":"e_1_2_14_22_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-03-643544"},{"key":"e_1_2_14_23_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2014.109892"},{"key":"e_1_2_14_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02177-w"},{"key":"e_1_2_14_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0051862"},{"key":"e_1_2_14_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14051335"},{"key":"e_1_2_14_27_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2020004144"},{"key":"e_1_2_14_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.18474"},{"key":"e_1_2_14_29_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2018-0827"},{"key":"e_1_2_14_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/petr.13825"},{"key":"e_1_2_14_31_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2021004367"},{"key":"e_1_2_14_32_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2021-145523"},{"key":"e_1_2_14_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-015-1877-y"},{"key":"e_1_2_14_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-019-0662-y"},{"key":"e_1_2_14_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40272-023-00588-5"},{"key":"e_1_2_14_36_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-01-619734"},{"key":"e_1_2_14_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-021-03248-x"},{"key":"e_1_2_14_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-021-01148-5"},{"key":"e_1_2_14_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3420"},{"key":"e_1_2_14_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00431-019-03468-8"},{"key":"e_1_2_14_41_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20180853"}],"container-title":["British Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bjh.19465","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T10:09:07Z","timestamp":1754993347000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bjh.19465"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,15]]},"references-count":40,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["10.1111\/bjh.19465"],"URL":"https:\/\/doi.org\/10.1111\/bjh.19465","archive":["Portico"],"relation":{},"ISSN":["0007-1048","1365-2141"],"issn-type":[{"value":"0007-1048","type":"print"},{"value":"1365-2141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,15]]},"assertion":[{"value":"2024-01-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}