{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T14:58:17Z","timestamp":1791644297229,"version":"4.3.4"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,4,26]],"date-time":"2024-04-26T00:00:00Z","timestamp":1714089600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2021A1515111034"],"award-info":[{"award-number":["2021A1515111034"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Primary immune thrombocytopenia (ITP) is linked to specific pathogenic mechanisms, yet its relationship with mitophagy and ferroptosis is poorly understood. This study aimed to identify new biomarkers and explore the role of mitophagy and ferroptosis in ITP pathogenesis. Techniques such as differential analysis, Mfuzz expression pattern clustering, machine learning, gene set enrichment analysis, single\u2010cell RNA sequencing (scRNA\u2010seq) and immune infiltration analysis were employed to investigate the molecular pathways of pivotal genes. Two\u2010sample Mendelian randomization (TSMR) assessed the causal effects in ITP. Key genes identified in the training set included <jats:italic>GABARAPL1<\/jats:italic>, <jats:italic>S100A8<\/jats:italic>, <jats:italic>LIN28A<\/jats:italic>, and <jats:italic>GDF9<\/jats:italic>, which demonstrated diagnostic potential in validation sets. Functional analysis indicated these genes' involvement in ubiquitin phosphorylation, PPAR signalling pathway and T\u2010cell differentiation. Immune infiltration analysis revealed increased macrophage presence in ITP, related to the critical genes. scRNA\u2010seq indicated reduced <jats:italic>GABARAPL1<\/jats:italic> expression in ITP bone marrow macrophages. TSMR linked <jats:italic>S100A8<\/jats:italic> with ITP diagnosis, presenting an OR of 0.856 (95% CI\u2009=\u20090.736\u20130.997, <jats:italic>p<\/jats:italic>\u2009=\u20090.045). The study pinpointed four central genes, <jats:italic>GABARAPL1<\/jats:italic>, <jats:italic>S100A8<\/jats:italic>, <jats:italic>LIN28A<\/jats:italic>, and <jats:italic>GDF9<\/jats:italic>, tied to mitophagy and ferroptosis in ITP. It posits that diminished <jats:italic>GABARAPL1<\/jats:italic> expression may disrupts ubiquitin phosphorylation and PPAR signalling, impairing mitophagy and inhibiting ferroptosis, leading to immune imbalance.<\/jats:p>","DOI":"10.1111\/bjh.19489","type":"journal-article","created":{"date-parts":[[2024,4,26]],"date-time":"2024-04-26T03:23:41Z","timestamp":1714101821000},"page":"2429-2441","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Comprehensive analysis and prediction model of mitophagy and ferroptosis in primary immune thrombocytopenia"],"prefix":"10.1111","volume":"204","author":[{"given":"Jiani","family":"Mo","sequence":"first","affiliation":[{"name":"Department of Hematology Affiliated Hospital of Guangdong Medical University (GDMU)  Zhanjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Liu","sequence":"additional","affiliation":[{"name":"Scientific Research 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