{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T09:33:33Z","timestamp":1781602413175,"version":"3.54.5"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T00:00:00Z","timestamp":1715126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005973","name":"Dr. Mildred Scheel Stiftung f\u00fcr Krebsforschung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005973","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009379","name":"Interdisziplin\u00e4res Zentrum f\u00fcr Klinische Forschung, Universit\u00e4tsklinikum W\u00fcrzburg","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009379","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Biomarkers for cytopenias following CAR T\u2010cell treatment in relapsed\/refractory (RR) multiple myeloma (MM) are not completely defined. We prospectively analysed 275 sequential peripheral blood (PB) samples from 58 RRMM patients treated with BCMA\u2010targeted CAR T cells, and then divided them into three groups: (i) baseline (before leukapheresis), (ii) \u2264day+30, and (iii) &gt;day+30 after CAR T\u2010cell therapy. We evaluated laboratory data and performed flow cytometry to determine the (CAR) T\u2010cell subsets. Baseline hyperferritinaemia was a risk factor for long\u2010lasting grade \u22653 anaemia (<jats:italic>r<\/jats:italic>\u2009=\u20090.47, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) and thrombocytopenia (<jats:italic>r<\/jats:italic>\u2009=\u20090.44, <jats:italic>p<\/jats:italic>\u2009=\u20090.002) after CAR T\u2010cell therapy. Low baseline haemoglobin (Hb) and PLT were associated with long\u2010lasting grade \u22653 anaemia (<jats:italic>r<\/jats:italic>\u2009=\u2009\u22120.56, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) and thrombocytopenia (<jats:italic>r<\/jats:italic>\u2009=\u2009\u22120.44, <jats:italic>p<\/jats:italic>\u2009=\u20090.002) respectively. We observed dynamics of CAR\u2010negative T\u2010cell subsets following CAR T\u2010cell infusion. In the late phase after CAR T\u2010cell therapy (&gt;day+30), CD4Tn frequency correlated with anaemia (<jats:italic>r<\/jats:italic>\u2009=\u20090.41, <jats:italic>p<\/jats:italic>\u2009=\u20090.0014) and lymphocytopenia was related to frequencies of CD8<jats:sup>+<\/jats:sup> T cells (<jats:italic>r<\/jats:italic>\u2009=\u20090.72, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) and CD8Teff (<jats:italic>r<\/jats:italic>\u2009=\u20090.64, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). CD4Tcm frequency was correlated with leucocytopenia (<jats:italic>r<\/jats:italic>\u2009=\u2009\u22120.49, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). In summary, preexisting cytopenias and hyperferritinaemia indicated long duration of grade \u22653 post\u2010CAR T\u2010cell cytopenias. Prolonged cytopenia may be related to immune remodelling with a shift in the CAR\u2010negative T\u2010cell subsets following CAR T\u2010cell therapy.<\/jats:p>","DOI":"10.1111\/bjh.19515","type":"journal-article","created":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T23:54:23Z","timestamp":1715212463000},"page":"999-1010","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Changes in T\u2010cell subsets, preexisting cytopenias and hyperferritinaemia correlate with cytopenias after <scp>BCMA<\/scp> targeted <scp>CAR<\/scp> T\u2010cell therapy in relapsed\/refractory multiple myeloma: Results from a prospective comprehensive biomarker study"],"prefix":"10.1111","volume":"205","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5772-7700","authenticated-orcid":false,"given":"Xiang","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Internal Medicine II University Hospital of W\u00fcrzburg  W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivien","family":"Wagner","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II University Hospital of W\u00fcrzburg  W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lukas","family":"Scheller","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II University Hospital of W\u00fcrzburg  W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilia","family":"Stanojkovska","sequence":"additional","affiliation":[{"name":"Mildred\u2010Scheel\u2010Nachwuchszentrum University Hospital of W\u00fcrzburg  W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Riedhammer","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II University Hospital of W\u00fcrzburg  W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianghui","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II University Hospital of W\u00fcrzburg  W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maximilian Johannes","family":"Steinhardt","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II University Hospital of W\u00fcrzburg  W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelia","family":"Vogt","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II University Hospital of W\u00fcrzburg  W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Nerreter","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II University Hospital of W\u00fcrzburg  W\u00fcrzburg 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