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Therefore, the present study aimed to examine the effect of thrombopoietin (TPO) on the recovery of BM EPCs of CIT patients and to identify the underlying mechanisms. The cell functions were determined by 1,1\u2018\u2010dioctadecyl\u20103,3,3\u2019,3\u2018\u2010tetramethylindocarbocyanine perchlorate (Dil)\u2013acetylated low\u2010density lipoprotein (Dil\u2010Ac\u2010LDL) uptake and fluorescein isothiocyanate (FITC)\u2010labeled Ulex europaeus agglutinin\u2010I (FITC\u2010UEA\u2010I) binding assay, as well as proliferation, migration and tube formation experiments. Endothelial cells were transfected with METTL16 lentivirus, followed by methylated RNA immunoprecipitation sequencing. Zebrafish with vascular defect was used as the in\u00a0vivo model. TPO significantly improved the quantity and functions of BM EPCs from CIT patients in\u00a0vitro and restored the subintestinal vein area of zebrafish with vascular defect in\u00a0vivo. Mechanically, TPO enhanced the BM EPC functions through Akt signal mediated by METTL16, which was downregulated in BM EPCs of CIT patients and involved in the regulation of endothelial functions. The present study demonstrates that TPO improves the recovery of BM EPCs from CIT patients with haematological malignancies via METTL16\/Akt signalling, which provides new insights into the role of TPO in treating CIT in addition to direct megakaryopoiesis.<\/jats:p>","DOI":"10.1111\/bjh.19722","type":"journal-article","created":{"date-parts":[[2024,8,27]],"date-time":"2024-08-27T04:19:03Z","timestamp":1724732343000},"page":"1532-1545","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Thrombopoietin improves the functions of bone marrow endothelial progenitor cells via <scp>METTL16<\/scp>\/Akt signalling of haematological patients with chemotherapy\u2010induced thrombocytopenia"],"prefix":"10.1111","volume":"205","author":[{"given":"Hui","family":"Chen","sequence":"first","affiliation":[{"name":"The Seventh Affiliated Hospital, Sun Yat\u2010Sen University  Shenzhen Guangdong P.R. 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