{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T22:34:06Z","timestamp":1773354846171,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,8,27]],"date-time":"2024-08-27T00:00:00Z","timestamp":1724716800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>To use proteomic techniques to identify sensitive diagnostic biomarkers for paediatric immune thrombocytopenia (ITP). We selected children in ITP and control groups, using a four\u2010dimensional data\u2010independent acquisition approach (4D\u2010DIA) to analyse its protein expression. The significantly differentially expressed proteins were selected for enzyme\u2010linked immunosorbent assay (ELISA) validation in a cohort comprising 50 samples (13 healthy controls, 15 secondary thrombocytopenia controls and 22 children with ITP). Receiver operating characteristics (ROC) were generated to diagnose ITP and to assess the diagnostic effectiveness of this approach. Compared with the control group, 55 differentially expressed proteins (43 increased and 12 decreased) were determined in the ITP group. Matrix metalloproteinases\u20109 (MMP\u20109) and thrombospondin\u20101 (THBS1) were significantly expressed and selected for ELISA. The verification outcomes aligned with the findings from the proteomic examinations. In contrast to the control cohort, the ITP subjects exhibited markedly elevated plasma MMP\u20109 levels and reduced plasma THBS1 concentrations. Additionally, the ROC curves indicated the diagnostic value of these biomarkers. In conclusion, proteomics facilitates identifying the sensitive biomarkers for ITP diagnosis. We have preliminarily selected two differentially expressed proteins, MMP\u20109 and THBS1, whose potential role as biomarkers for diagnosing ITP requires further research.<\/jats:p>","DOI":"10.1111\/bjh.19730","type":"journal-article","created":{"date-parts":[[2024,8,27]],"date-time":"2024-08-27T04:15:17Z","timestamp":1724732117000},"page":"1921-1929","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Study on diagnostic\u2010sensitive markers of primary immune thrombocytopenia in children based on plasma proteomics"],"prefix":"10.1111","volume":"205","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-5808-5825","authenticated-orcid":false,"given":"Wei","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Pediatrics The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University  Huai'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University  Huai'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingqing","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Pediatrics The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University  Huai'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanyuan","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Pediatrics The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University  Huai'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiyan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University  Huai'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rongrong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University  Huai'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaofang","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Neonatology The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University  Huai'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3520-4773","authenticated-orcid":false,"given":"Yufang","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Pediatrics The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University  Huai'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,8,27]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12098-019-03155-4"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2021.100822"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation-2015.1.237"},{"issue":"11","key":"e_1_2_13_5_1","article-title":"Pathogenesis and therapeutic mechanisms in immune thrombocytopenia (ITP)","volume":"64","author":"Zufferey A","year":"2017","journal-title":"J Clin Med"},{"issue":"10","key":"e_1_2_13_6_1","first-page":"810","article-title":"Adapted guideline for the diagnosis and treatment of primary immune thrombocytopenia for Chinese children (2021)","volume":"59","author":"Working Group of Chinese Guideline for the Diagnosis and Treatment of Childhood Primary Immune thrombocytopenia; 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