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Using an Olink proteomics platform with a 92 immune response\u2010related human protein panel, we analysed plasma samples from ITP patients (ITP, <jats:italic>n<\/jats:italic>\u2009=\u200940), patients with thrombocytopaenia secondary to other causes (Non\u2010ITP, <jats:italic>n<\/jats:italic>\u2009=\u200919) and healthy controls (NC, <jats:italic>n<\/jats:italic>\u2009=\u200918), of a discovery cohort as well as a validation cohort (ITP, <jats:italic>n<\/jats:italic>\u2009=\u200936; NC, <jats:italic>n<\/jats:italic>\u2009=\u200920). A total of 10 differentially expressed proteins (DEPs) were identified in the ITP group compared with the non\u2010ITP and NC groups of the discovery cohort. These include CXCL11, GZMH, ARG1, TGF\u2010\u03b21, ANGPT1, CXCL12, CD40\u2010L, PDGF subunit B, IL4 and TNFSF14. Furthermore, least absolute shrinkage and selection operator regression analysis showed some of these DEPs, such as CXCL11, TGF\u2010\u03b21, ARG1 and GZMH to be significant in differentiating between patients with ITP and healthy controls (validation area under the curve\u2009=\u20090.87). The analysis demonstrated that the ITP group has a specific proteomic profile relative to non\u2010ITP and NC groups. In summary, we report for the first time that Olink precision proteomics can specifically detect up\u2010regulated inflammatory proteins as potential diagnostic biomarkers for ITP.<\/jats:p>","DOI":"10.1111\/bjh.19760","type":"journal-article","created":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T05:05:02Z","timestamp":1727154302000},"page":"133-143","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Targeted proteomics profiling reveals valuable biomarkers in the diagnosis of primary immune thrombocytopaenia"],"prefix":"10.1111","volume":"206","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9386-3678","authenticated-orcid":false,"given":"Yizhi","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Hematology The First Affiliated Hospital of Wannan Medical College  Wuhu China"},{"name":"NHC Key Laboratory of Thrombosis and Hemostasis, Jiangsu Institute of Hematology The First Affiliated 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