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This study investigates the detection of <jats:italic>CAR<\/jats:italic> copies in circulating cell\u2010free DNA (cfDNA) as a potential predictive biomarker of early relapse (&lt;6\u2009months) to improve patient management. In this research, we have consecutively selected 73 ABCL patients treated with anti\u2010CD19 CAR T\u2010cells, analysing <jats:italic>CAR<\/jats:italic> levels in peripheral blood and other clinical variables. Our results indicate that no correlation is present between genomic DNA and cfDNA; moreover, higher levels of <jats:italic>CAR<\/jats:italic>\u2010cfDNA on day +14 after infusion (0.44 vs. 0.07; <jats:italic>p<\/jats:italic>\u2009=\u20090.019) are associated with improved 6\u2010month progression\u2010free survival rates (74.2% vs. 26%. <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01), suggesting that <jats:italic>CAR<\/jats:italic>\u2010cfDNA could be a strong predictor of CAR T\u2010cell therapy short\u2010term outcomes. These findings underscore the potential of integrating <jats:italic>CAR<\/jats:italic>\u2010cfDNA analysis into routine clinical practice to enhance the prognostic accuracy and therapeutic strategies for ABCL patients undergoing CAR T\u2010cell therapy.<\/jats:p>","DOI":"10.1111\/bjh.19916","type":"journal-article","created":{"date-parts":[[2024,12,13]],"date-time":"2024-12-13T06:03:47Z","timestamp":1734069827000},"page":"195-203","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Chimeric antigen receptor copies in cell\u2010free <scp>DNA<\/scp> predict relapse in aggressive B\u2010cell lymphoma patients treated with <scp>CAR<\/scp> T\u2010cell therapy"],"prefix":"10.1111","volume":"206","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-2111-9719","authenticated-orcid":false,"given":"Ismael","family":"de la Iglesia\u2010San Sebasti\u00e1n","sequence":"first","affiliation":[{"name":"Department of Hematology Hospital 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