{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T12:17:11Z","timestamp":1767183431660,"version":"build-2065373602"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,4,20]],"date-time":"2025-04-20T00:00:00Z","timestamp":1745107200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000009","name":"Foundation for the National Institutes of Health","doi-asserted-by":"publisher","award":["2P50CA100707\u201016A1"],"award-info":[{"award-number":["2P50CA100707\u201016A1"]}],"id":[{"id":"10.13039\/100000009","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005189","name":"Leukemia and Lymphoma Society","doi-asserted-by":"publisher","award":["6673\u201024"],"award-info":[{"award-number":["6673\u201024"]}],"id":[{"id":"10.13039\/100005189","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Cell lines have enabled a comprehensive understanding of disease biology and the advancement of new therapeutics in Waldenstr\u00f6m macroglobulinaemia (WM). Herein, we report the development of BCWM.2, a novel WM cell line derived from an untreated symptomatic WM patient. Immunophenotypic and genomic analyses confirmed its origin from primary bone marrow WM cells. Flow cytometry revealed expression of CD19, CD20, CD23, CD38, CD45RA, CD52, immunoglobulin M (IgM) heavy chain and \u03bb light chain on BCWM.2 cells. Whole genome sequencing demonstrated that, unlike BCWM.1, MWCL\u20101 and RPCI\u2010WM1, which harbour <jats:italic>MYD88<\/jats:italic><jats:sup><jats:italic>L265P<\/jats:italic><\/jats:sup> mutations, BCWM.2 carries <jats:italic>MYD88<\/jats:italic><jats:sup><jats:italic>S243N<\/jats:italic><\/jats:sup>. BCWM.2 also exhibited hallmark WM genetic aberrations, including 6q deletion and 6p amplification, along with unique mutations in <jats:italic>LYN, AKAP9, HDAC5, RUNX3<\/jats:italic> and <jats:italic>SPI1<\/jats:italic>. BCWM.2 cells exhibited optimal growth when co\u2010cultured with HS\u20105 stromal cells and developed subcutaneous flank masses in all five NOD\u2010SCID mice, along with measurable serum human IgM levels. BCWM.2 cells also showed remarkable sensitivity to the B\u2010cell lymphoma 2 inhibitor venetoclax and the Janus kinase 2\/Interleukin\u20101 receptor\u2010associated kinase 1 inhibitor pacritinib, underscoring their utility for identification of pharmacologically active agents. BCWM.2 represents a novel myeloid differentiation primary response 88\u2010mutated, 6q\u2010deleted cell line with distinct genomic features for in\u00a0vivo and in\u00a0vitro studies for WM.<\/jats:p>","DOI":"10.1111\/bjh.20072","type":"journal-article","created":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T09:24:50Z","timestamp":1748337890000},"page":"1604-1614","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Development and characterization of the novel <i>MYD88<\/i> mutated, 6q deleted <scp>BCWM<\/scp>.2 cell line for Waldenstr\u00f6m macroglobulinaemia"],"prefix":"10.1111","volume":"206","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3821-9134","authenticated-orcid":false,"given":"Shirong","family":"Liu","sequence":"first","affiliation":[{"name":"Bing Center for Waldenstr\u00f6m Macroglobulinemia Dana\u2010Farber Cancer Institute  Boston Massachusetts USA"},{"name":"Department of Medicine Harvard 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and Belfer Center for Applied Cancer Science Dana\u2010Farber Cancer Institute  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johany","family":"Penailillo","sequence":"additional","affiliation":[{"name":"Department of Oncologic Pathology, Dana\u2010Farber Cancer Institute Harvard Medical School  Boston Massachusetts USA"},{"name":"Department of Pathology, Brigham and Women's Hospital Harvard Medical School  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexa G.","family":"Canning","sequence":"additional","affiliation":[{"name":"Bing Center for Waldenstr\u00f6m Macroglobulinemia Dana\u2010Farber Cancer Institute  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Cao","sequence":"additional","affiliation":[{"name":"Bing Center for Waldenstr\u00f6m Macroglobulinemia Dana\u2010Farber Cancer Institute  Boston Massachusetts 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for Waldenstr\u00f6m Macroglobulinemia Dana\u2010Farber Cancer Institute  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruben D.","family":"Carrasco","sequence":"additional","affiliation":[{"name":"Department of Oncologic Pathology, Dana\u2010Farber Cancer Institute Harvard Medical School  Boston Massachusetts USA"},{"name":"Department of Pathology, Brigham and Women's Hospital Harvard Medical School  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prafulla C.","family":"Gokhale","sequence":"additional","affiliation":[{"name":"Experimental Therapeutics Core and Belfer Center for Applied Cancer Science Dana\u2010Farber Cancer Institute  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0075-6735","authenticated-orcid":false,"given":"Shayna R.","family":"Sarosiek","sequence":"additional","affiliation":[{"name":"Bing Center for 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