{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T05:17:33Z","timestamp":1790572653488,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,5,25]],"date-time":"2025-05-25T00:00:00Z","timestamp":1748131200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai Municipality","doi-asserted-by":"publisher","award":["19ZR1410000"],"award-info":[{"award-number":["19ZR1410000"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Primary immune thrombocytopenia (ITP) is a condition characterized by immune\u2010mediated platelet loss due to excessive destruction and\/or insufficient production. IL\u201012p35 is involved in autoimmune uveitis; however, its role in ITP remains unknown. In the study, CD19<jats:sup>+<\/jats:sup> B and CD4<jats:sup>+<\/jats:sup> T cells were isolated from peripheral blood mononuclear cells (PBMCs) of patients with ITP and healthy individuals. CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>\u2212<\/jats:sup> T cells were cocultured with the CD19<jats:sup>+<\/jats:sup> B cells treated with anti\u2010IL\u201012p35 antibody or IL\u201012p35 recombinant protein. The impact of IL\u201012p35 on CD19<jats:sup>+<\/jats:sup> B cells was assessed by enzyme\u2010linked immunosorbent assay, Western blotting, quantitative real\u2010time PCR and flow cytometry. PBMCs from patients with ITP showed lower proportions of IL\u201010<jats:sup>+<\/jats:sup>CD19<jats:sup>+<\/jats:sup> B cells and decreased mRNA levels of IL\u201012p35\u2010coding gene <jats:italic>IL12A<\/jats:italic> than those from healthy individuals. Anti\u2010IL\u201012p35 antibody\u2010treated CD19<jats:sup>+<\/jats:sup> B cells could reduce the population of IL\u201010<jats:sup>+<\/jats:sup> in CD19<jats:sup>+<\/jats:sup> B cells and regulate the differentiation of CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>\u2212<\/jats:sup> T cells, while recombinant IL\u201012p35 demonstrated the opposite effects. Moreover, the increased IL\u201010<jats:sup>+<\/jats:sup> B\u2010cell population and HIF\u20101\u03b1 expression in CD19<jats:sup>+<\/jats:sup> B cells induced by recombinant IL\u201012p35 were reversed by HIF\u20101\u03b1 and JAK2\/STAT3 inhibitors. In conclusion, CD19<jats:sup>+<\/jats:sup>IL\u201010<jats:sup>+<\/jats:sup> B cells induced by IL\u201012p35 regulate CD4<jats:sup>+<\/jats:sup> T\u2010cell subsets in patients with ITP via the JAK2\/STAT3\/HIF\u20101\u03b1 signalling pathway.<\/jats:p>","DOI":"10.1111\/bjh.20173","type":"journal-article","created":{"date-parts":[[2025,5,26]],"date-time":"2025-05-26T02:18:03Z","timestamp":1748225883000},"page":"369-380","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>CD19<\/scp><sup>+<\/sup><scp>IL<\/scp>\u201010<sup>+<\/sup> regulatory B cells induced by <scp>IL<\/scp>\u201012p35 regulates functional T\u2010cell subsets in patients with immune thrombocytopenia"],"prefix":"10.1111","volume":"207","author":[{"given":"Fanli","family":"Hua","sequence":"first","affiliation":[{"name":"Department of Hematology Zhongshan Hospital, Qingpu Branch, Fudan University  Shanghai 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