{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T14:55:01Z","timestamp":1766415301507,"version":"3.44.0"},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T00:00:00Z","timestamp":1752019200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Efficacy of chimeric antigen receptor (CAR) T\u2010cell therapy hinges on CAR\u2010T potency, as well as on tumour traits and disease status. Potency assessment currently relies on post\u2010infusion in\u00a0vivo growth; therefore, manufacturing metrics could provide early potency predictions, enabling potency\u2010guided strategies. To assess the impact of ex\u00a0vivo cell growth during manufacturing on clinical outcomes, we analysed diffuse large B\u2010cell lymphoma patients treated with tisagenlecleucel, merging clinical records with manufacturing parameters. Of 75 cases, 21 (28%) showed poor growth, indicated by any decrease in cell number during manufacturing. Good growth correlated with significantly better overall response rate (85.2% vs. 52.4%; <jats:italic>p<\/jats:italic>\u2009=\u20090.006), with enhanced lymphocyte increase in peripheral blood after infusion. Multivariate analysis revealed that this group had significantly higher progression\u2010free survival (PFS) (adjusted hazard ratio [aHR]: 0.377; 95% confidence interval [CI]: 0.180\u20130.789; <jats:italic>p<\/jats:italic>\u2009=\u20090.010) and overall survival (OS) (aHR: 0.191; 95% CI: 0.071\u20130.510; <jats:italic>p<\/jats:italic>\u2009=\u20090.001), with lower cumulative incidence of disease progression (aHR: 0.412; 95% CI: 0.198\u20130.858; <jats:italic>p<\/jats:italic>\u2009=\u20090.018), compared to the poor growth group, even after adjusting for clinical factors, bridging therapies performed between apheresis and infusion and disease status at infusion. Our findings suggest that good cell growth during manufacturing predicts favourable post\u2010CAR\u2010T outcomes. Identifying clinical factors that influence manufacturing parameters could improve post\u2010CAR\u2010T outcomes.<\/jats:p>","DOI":"10.1111\/bjh.20266","type":"journal-article","created":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T00:34:37Z","timestamp":1752107677000},"page":"1002-1010","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Consistent ex\u00a0vivo cell proliferation during manufacturing predicts favourable outcomes post\u2010<scp>CAR<\/scp>\u2010T\u2010cell therapy"],"prefix":"10.1111","volume":"207","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9381-0421","authenticated-orcid":false,"given":"Tomoyasu","family":"Jo","sequence":"first","affiliation":[{"name":"Department of Hematology Kyoto University Hospital  Kyoto Japan"},{"name":"Department of Cytotherapy Kyoto University Hospital  Kyoto Japan"},{"name":"Department of Clinical Laboratory Medicine and Center for Research and Application of 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