{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T23:34:32Z","timestamp":1777592072812,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T00:00:00Z","timestamp":1756339200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The IKAROS (<jats:italic>IKZF1<\/jats:italic>) transcription factor regulates the differentiation of pre\u2010B cells to mature B lymphocytes. The loss of <jats:italic>IKZF1<\/jats:italic> expression has been linked with increased matrix adhesion along with increased expression of stemness markers that form a hallmark of B\u2010lymphoblasts. However, the spectrum of differentially regulated genes has not been investigated in detail in B\u2010cell acute lymphoblastic leukaemia (B\u2010ALL) patients expressing <jats:italic>IKZF1<\/jats:italic> deletion. In this study, we analysed the gene expression signature of 24 B\u2010ALL patients with <jats:italic>IKZF1<\/jats:italic> deletion against 32 B\u2010ALL patients with wild\u2010type <jats:italic>IKZF1<\/jats:italic>. RNA sequencing revealed an adhesion signature corresponding to deregulation of <jats:italic>MUC4<\/jats:italic>, secreted phosphoprotein 1 (<jats:italic>SPP1<\/jats:italic>), <jats:italic>LAMB3<\/jats:italic>, <jats:italic>CLSTN2<\/jats:italic> and <jats:italic>MERTK<\/jats:italic>. The upregulation of <jats:italic>MUC4<\/jats:italic> expression was further validated in a B\u2010ALL patient cohort. Moreover, by chromatin immunoprecipitation, we observed the direct binding of <jats:italic>IKZF1<\/jats:italic> on <jats:italic>MUC4<\/jats:italic> promoter and enhancer sequences. Thus, the increase in <jats:italic>MUC4<\/jats:italic> expression upon <jats:italic>IKZF1<\/jats:italic> deletion could be explained by a loss of <jats:italic>IKZF1<\/jats:italic> repression on the <jats:italic>MUC4<\/jats:italic> locus. Finally, using the TARGET\u2010ALL\u2010P2 database, we observed a correlation between decreased survival and high <jats:italic>MUC4<\/jats:italic> expression in B\u2010ALL patients. Thus, we propose <jats:italic>MUC4<\/jats:italic> expression as an indicator of patient prognosis that correlates with the loss of <jats:italic>IKZF1<\/jats:italic> in B\u2010ALL.<\/jats:p>","DOI":"10.1111\/bjh.70014","type":"journal-article","created":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T05:55:58Z","timestamp":1756446958000},"page":"1259-1270","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Gene expression signature of <scp><i>IKZF1<\/i><\/scp> deleted B\u2010cell acute lymphoblastic leukaemia reveals a unique regulation of <scp><i>MUC4<\/i><\/scp>"],"prefix":"10.1111","volume":"207","author":[{"given":"Preity","family":"Sharma","sequence":"first","affiliation":[{"name":"Laboratory Oncology Unit, Dr BRA IRCH All India Institute of Medical Sciences (AIIMS)  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gadha 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