{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T16:39:30Z","timestamp":1782837570070,"version":"3.54.5"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,8,8]],"date-time":"2025-08-08T00:00:00Z","timestamp":1754611200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82000131"],"award-info":[{"award-number":["82000131"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82341213"],"award-info":[{"award-number":["82341213"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010041","name":"Tianjin Municipal Science and Technology Committee","doi-asserted-by":"publisher","award":["23JCYBJC01050"],"award-info":[{"award-number":["23JCYBJC01050"]}],"id":[{"id":"10.13039\/501100010041","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010041","name":"Tianjin Municipal Science and Technology Committee","doi-asserted-by":"publisher","award":["23JCYBJC01060"],"award-info":[{"award-number":["23JCYBJC01060"]}],"id":[{"id":"10.13039\/501100010041","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFC2500300"],"award-info":[{"award-number":["2021YFC2500300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2025,10]]},"abstract":"Summary<\/jats:title>The t(8;21)(q22;q22) translocation is one of the most recurrent cytogenetic aberrations in acute myeloid leukaemia (AML). While most patients achieve complete remission, approximately 40% of them still relapse. Early identification and elimination of leukaemia clones with relapse potential could improve prognosis for t(8;21) AML patients. Here, through single\u2010cell RNA sequencing, we characterized the intra\u2010tumoral heterogeneity of t(8;21) AML and identified haematopoietic stem cell (HSC)\u2010like subset as the most quiescent and primitive subgroup among all leukaemia cell populations. Further investigations revealed IKZF2<\/jats:italic> as the master regulator for HSC\u2010like subset. Notably, IKZF2<\/jats:italic> was upregulated in t(8;21) AML compared with other AML subtypes and was specifically targeted by AML1\u2010ETO. Using primary samples and mouse models, we verified the high enrichment of IKZF2<\/jats:italic> in primitive and quiescent leukaemic cells. Moreover, IKZF2<\/jats:italic> knockout hindered the accumulation of aberrant stem cells driven by AML1\u2010ETO and promoted cellular differentiation both in\u00a0vitro and in\u00a0vivo. These facilitate a better understanding of the leukaemia cell heterogeneity in t(8;21) AML and unveil IKZF2<\/jats:italic> as a potential target for improving current treatment strategies.<\/jats:p>","DOI":"10.1111\/bjh.70077","type":"journal-article","created":{"date-parts":[[2025,8,9]],"date-time":"2025-08-09T05:44:32Z","timestamp":1754718272000},"page":"1309-1322","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Single\u2010cell transcriptome profiling reveals blast cell heterogeneity and identifies novel therapeutic target IKZF2<\/scp> in t(8;21) acute myeloid leukaemia"],"prefix":"10.1111","volume":"207","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-1979-7713","authenticated-orcid":false,"given":"Yu","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Tianjin China"},{"name":"Tianjin Institutes of Health Science Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbing","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Tianjin China"},{"name":"Tianjin Institutes of Health Science Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5438-2544","authenticated-orcid":false,"given":"Yihan","family":"Mei","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Tianjin China"},{"name":"Tianjin Institutes of Health Science Tianjin China"},{"name":"Department of Hematology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital University of Electronic Science and Technology of China Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianqian","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Tianjin China"},{"name":"Tianjin Institutes of Health Science Tianjin 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