{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T20:18:18Z","timestamp":1789330698876,"version":"build-2803163510"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T00:00:00Z","timestamp":1756425600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100024250","name":"Israel Innovation Authority","doi-asserted-by":"publisher","award":["68113"],"award-info":[{"award-number":["68113"]}],"id":[{"id":"10.13039\/501100024250","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>Traditional microscopy compromises between resolution and field of view, limiting its diagnostic sensitivity. We present here next\u2010generation morphology (NGM), a novel five\u2010layer analytical approach leveraging a physical solution that enables high\u2010resolution, large\u2010field digital imaging, data mining and analysis of blood specimens. To test NGM performance, peripheral blood smears (PBS) from 37 myelodysplastic syndrome (MDS) patients and 30 age\u2010matched controls were analysed. PBS topology was mapped to identify regions optimal for analysis. Red blood cells (RBCs) displayed a gradient distribution with a narrow zone suitable for accurate morphological assessment. NGM facilitated direct morphometric measurements of individual red and white blood cells (RBCs and WBCs), which were correlated with clinical variables. Significant morphometric differences were found between MDS and controls, particularly in RBC size, shape and neutrophil granularity and subpopulations profile. WBC differentials based on 1000 WBC significantly elevated blasts detection compared to conventional 100\u2010WBC counts. Multivariate analysis linked RBC morphometrics with high\u2010risk MDS. In summary, NGM overcomes traditional microscopy limitations, offering high\u2010resolution, large\u2010field imaging for sensitive digital morphometry of PBS. This technology can enhance the detection and quantitative characterization of haematological abnormalities, thereby contributing to the understanding of MDS physiology, and may support early detection of dysplasia in PBS.<\/jats:p>","DOI":"10.1111\/bjh.70110","type":"journal-article","created":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T10:02:03Z","timestamp":1756461723000},"page":"2379-2390","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Next\u2010generation morphology, a novel multilayer morphometric digital analysis, reveals both the basic topology and new trends of myelodysplasia of peripheral blood specimens"],"prefix":"10.1111","volume":"207","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5058-943X","authenticated-orcid":false,"given":"Ben\u2010Zion","family":"Katz","sequence":"first","affiliation":[{"name":"Division of Hematology Tel Aviv Sourasky Medical Center  Tel Aviv Israel"},{"name":"Division of Clinical Laboratories Tel Aviv Sourasky Medical Center  Tel Aviv Israel"},{"name":"Faculty of Medicine Tel Aviv University  Tel Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yakir","family":"Moshe","sequence":"additional","affiliation":[{"name":"Division of Hematology Tel Aviv Sourasky Medical Center  Tel Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Benisty","sequence":"additional","affiliation":[{"name":"Division of Hematology Tel Aviv Sourasky Medical Center  Tel Aviv Israel"},{"name":"Division of Clinical Laboratories Tel Aviv Sourasky Medical Center  Tel Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0212-2135","authenticated-orcid":false,"given":"Efrat","family":"Luttwak","sequence":"additional","affiliation":[{"name":"Division of Hematology Tel Aviv Sourasky Medical Center  Tel Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Merav","family":"Brazilai","sequence":"additional","affiliation":[{"name":"Division of Hematology Tel Aviv Sourasky Medical Center  Tel Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1936-4675","authenticated-orcid":false,"given":"Howard S.","family":"Oster","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Tel Aviv University  Tel Aviv Israel"},{"name":"Division of Internal Medicine Tel Aviv Sourasky Medical Center  Tel Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Pozdnyakova","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Hospital of the University of Pennsylvania  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moshe","family":"Mittelman","sequence":"additional","affiliation":[{"name":"Division of Hematology Tel Aviv Sourasky Medical Center  Tel Aviv Israel"},{"name":"Faculty of Medicine Tel Aviv University  Tel Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8663-444X","authenticated-orcid":false,"given":"Irit","family":"Avivi","sequence":"additional","affiliation":[{"name":"Division of Hematology Tel Aviv Sourasky Medical Center  Tel Aviv Israel"},{"name":"Faculty of Medicine Tel Aviv University  Tel Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,29]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2020.100732"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.19.01231"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-021-01861-y"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2020010568"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12818"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-39676-y"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.13042"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2005.035402"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqae091"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.13681"},{"issue":"3","key":"e_1_2_12_12_1","first-page":"279CT","article-title":"Computed tomography: a technical review","volume":"89","author":"Seeram E","year":"2018","journal-title":"Radiol Technol"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1364\/AO.21.002758"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-03-420489"},{"key":"e_1_2_12_15_1","volume-title":"Reference leukocyte (WBC) differential count (proportional) and evaluation of instrumental methods; approved standard","author":"Clinical and Laboratory Standard Institute","year":"2007"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra043442"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-05-1615"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.448280"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1055\/a-1957-6219"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/9934144"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0261848"},{"key":"e_1_2_12_22_1","volume-title":"Blood cells; a practical guide","author":"Bain BJ","year":"2015"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMp048266"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0000000000000325"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2014.03.036"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.19599"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.602963"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.4110\/in.2017.17.5.298"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/107.5.582"},{"key":"e_1_2_12_30_1","first-page":"39","volume-title":"Differential leukocyte counting","author":"Rumke CL","year":"1977"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2022008967"}],"container-title":["British Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bjh.70110","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T11:44:18Z","timestamp":1767959058000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bjh.70110"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,29]]},"references-count":30,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1111\/bjh.70110"],"URL":"https:\/\/doi.org\/10.1111\/bjh.70110","archive":["Portico"],"relation":{},"ISSN":["0007-1048","1365-2141"],"issn-type":[{"value":"0007-1048","type":"print"},{"value":"1365-2141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,29]]},"assertion":[{"value":"2025-05-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}