{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T03:08:15Z","timestamp":1787800095454,"version":"build-2784847793"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T00:00:00Z","timestamp":1756857600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T00:00:00Z","timestamp":1756857600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100007394","name":"Association Laurette Fugain","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007394","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Measurable residual disease (MRD) is a strong prognostic factor in acute myeloid leukaemia (AML). Next\u2010generation sequencing (NGS) offers promise but must distinguish true signal from background. We assessed MRD in 98 adult AML patients in first complete remission after intensive chemotherapy using a duplex unique molecular identifier (UMI)\u2010based NGS capture panel. Error reduction analysis showed up to a 20\u2010fold decrease in artefactual calls versus conventional sequencing. Linearity studies with serial dilutions confirmed accurate quantification down to 0.01% variant allele frequency. In this cohort, NGS\u2010MRD positivity did not significantly affect overall survival (OS) or relapse\u2010free survival (RFS) after one course of chemotherapy. However, NGS\u2010MRD positivity &gt;0.1%, excluding\n                    <jats:italic>DNMT3A<\/jats:italic>\n                    ,\n                    <jats:italic>TET2<\/jats:italic>\n                    ,\n                    <jats:italic>ASXL1<\/jats:italic>\n                    ,\n                    <jats:italic>IDH1<\/jats:italic>\n                    and\n                    <jats:italic>IDH2<\/jats:italic>\n                    mutations, was significantly associated with inferior outcomes after two courses (OS: hazard ratio [HR]\u2009=\u20093.04,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.0173; RFS: HR\u2009=\u20092.83,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.0097). Combining multiparameter flow cytometry (MFC\u2010MRD) with NGS\u2010MRD identified a double\u2010positive subgroup with particularly poor outcomes after the first course (OS: HR\u2009=\u20097.98,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001; RFS: HR\u2009=\u20097.87,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). These findings underscore that duplex UMI\u2010based NGS is a sensitive, quantitative approach for MRD assessment in AML, offering prognostic information complementary to MFC\u2010MRD.\n                  <\/jats:p>","DOI":"10.1111\/bjh.70135","type":"journal-article","created":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T03:24:36Z","timestamp":1756956276000},"page":"1953-1961","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Monitoring of measurable residual disease by next\u2010generation sequencing in patients with acute myeloid leukaemia"],"prefix":"10.1111","volume":"207","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9154-1085","authenticated-orcid":false,"given":"Augustin","family":"Boudry","sequence":"first","affiliation":[{"name":"Laboratory of Hematology, Biology and Pathology Center CHU Lille  Lille France"},{"name":"University of Lille, ULR 2694\u2013Metrics  Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laure","family":"Goursaud","sequence":"additional","affiliation":[{"name":"Department of Hematology Claude Huriez Hospital, CHU Lille  Lille France"},{"name":"University of Lille, U1277\u2010Canther, INSERM  Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphine","family":"Lebon","sequence":"additional","affiliation":[{"name":"Department of Hematology and Cell Therapy CHU Amiens\u2010Picardie  Amiens France"},{"name":"University of Picardie Jules Verne, EA4666\u2013HEMATIM  Amiens France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florent","family":"Dumezy","sequence":"additional","affiliation":[{"name":"Laboratory of Hematology, Biology and Pathology Center CHU Lille  Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e9ronique","family":"Harrivel","sequence":"additional","affiliation":[{"name":"Laboratory of Hematology CHU Amiens\u2010Picardie  Amiens France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Boue\u2010Raguet","sequence":"additional","affiliation":[{"name":"Department of Hematology and Cell Therapy CHU Amiens\u2010Picardie  Amiens France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romane","family":"Joudinaud","sequence":"additional","affiliation":[{"name":"Laboratory of Hematology, Biology and Pathology Center CHU Lille  Lille France"},{"name":"University of Lille, U1277\u2010Canther, INSERM  Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice","family":"Marceau\u2010Renaut","sequence":"additional","affiliation":[{"name":"Laboratory of Hematology, Biology and Pathology Center CHU Lille  Lille France"},{"name":"University of Lille, U1277\u2010Canther, INSERM  Lille 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Center CHU Lille  Lille France"},{"name":"University of Lille, U1277\u2010Canther, INSERM  Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9line","family":"Berthon","sequence":"additional","affiliation":[{"name":"Department of Hematology Claude Huriez Hospital, CHU Lille  Lille France"},{"name":"University of Lille, U1277\u2010Canther, INSERM  Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Pierre","family":"Marolleau","sequence":"additional","affiliation":[{"name":"Department of Hematology and Cell Therapy CHU Amiens\u2010Picardie  Amiens France"},{"name":"University of Picardie Jules Verne, EA4666\u2013HEMATIM  Amiens France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Figeac","sequence":"additional","affiliation":[{"name":"University of Lille, US 41\u2013UAR 2014\u2013PLBS, CNRS, INSERM, CHU Lille, Institut Pasteur de Lille  Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude","family":"Preudhomme","sequence":"additional","affiliation":[{"name":"Laboratory of Hematology, Biology and Pathology Center CHU Lille  Lille France"},{"name":"University of Lille, U1277\u2010Canther, INSERM  Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3927-1022","authenticated-orcid":false,"given":"Nicolas","family":"Duployez","sequence":"additional","affiliation":[{"name":"Laboratory of Hematology, Biology and Pathology Center CHU Lille  Lille France"},{"name":"University of Lille, U1277\u2010Canther, INSERM  Lille 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