{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T17:00:20Z","timestamp":1787590820269,"version":"build-2736575974"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T00:00:00Z","timestamp":1773446400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T00:00:00Z","timestamp":1773446400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["CA076167"],"award-info":[{"award-number":["CA076167"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    A truncated derivative of Fibroblast growth factor receptor\u20101 (FGFR1) (tnFGFR1) independently transforms haematopoietic stem cells leading to a stem cell\u2010like leukaemia\/lymphoma syndrome (SCLL). In mouse models, these transformed cells show extensive genetic reprogramming that is distinct from cells transformed with full\u2010length chimeric FGFR1 kinases. We now show that the truncated derivative is insensitive to kinase inhibitors, and its increased expression is related to resistance to kinase inhibitors. Chromatin immunoprecipitation (ChIP) analysis shows that tnFGFR1 can occupy the promoters of the\n                    <jats:italic>Myc<\/jats:italic>\n                    ,\n                    <jats:italic>Flt3<\/jats:italic>\n                    and\n                    <jats:italic>Kit<\/jats:italic>\n                    genes suggesting a transcription regulatory function. However, without a deoxyribonucleic acid\u2010binding motif, tnFGFR1 must interact with binding partners that supply this function. To define this potential regulatory complex, we performed immunoprecipitation\u2010mass spectroscopy (IP\u2010MS) and demonstrate that tnFGFR1 is present in a complex with the splicing factor proline\u2010 and glutamine\u2010rich (SFPQ) protein and non\u2010POU domain\u2010containing octamer\u2010binding protein (NONO) protein. In addition, this protein complex is present on the promoters of target genes\n                    <jats:italic>Flt3<\/jats:italic>\n                    and\n                    <jats:italic>Kit<\/jats:italic>\n                    , and knockdown of either SFPQ or NONO prevents activation of their target genes. In addition, treatment with the NONO inhibitor auranofin suppresses cell proliferation of tnFGFR1\u2010transformed cells in\u00a0vitro mitigating leukaemia progression in\u00a0vivo in a mouse model. Thus, future targeting of this tnFGFR1 transcription complex may provide a means for treating tnFGFR1\u2010driven leukaemia.\n                  <\/jats:p>","DOI":"10.1111\/bjh.70426","type":"journal-article","created":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T09:41:12Z","timestamp":1773481272000},"page":"1564-1571","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["An oncogenic, truncated\n                    <scp>FGFR1<\/scp>\n                    variant cooperates with\n                    <scp>SPFQ<\/scp>\n                    \/\n                    <scp>NONO<\/scp>\n                    to regulate gene transcription in\n                    <scp>FGFR1<\/scp>\n                    \u2010driven leukaemia"],"prefix":"10.1111","volume":"208","author":[{"given":"John K.","family":"Cowell","sequence":"first","affiliation":[{"name":"Georgia Cancer Center  Augusta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuexiu","family":"Fang","sequence":"additional","affiliation":[{"name":"Georgia Cancer Center  Augusta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie F.","family":"Mori","sequence":"additional","affiliation":[{"name":"Georgia Cancer Center  Augusta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianxiang","family":"Hu","sequence":"additional","affiliation":[{"name":"Georgia Cancer Center  Augusta Georgia 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