{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T12:54:28Z","timestamp":1781528068000,"version":"3.54.1"},"reference-count":60,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T00:00:00Z","timestamp":1775692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T00:00:00Z","timestamp":1775692800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100018996","name":"Hellenic Academic Libraries Link","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100018996","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Omics technologies have transformed research in haemoglobinopathies, yet the proteome of RBCs remains largely unexplored in transfusion\u2010dependent thalassaemia (TDT). In this proteomic analysis, Red blood cell (RBC) membranes from 48 adults with TDT were compared with healthy controls. The resulting profile, corresponding to patient and donor RBCs, captured disease\u2010linked features and exposure to donor blood. Certain differentially expressed proteins were plasma\u2010absorbed or remnants of erythroid precursors, recapitulating findings in non\u2010transfusion\u2010dependent thalassaemia. Structural proteins were overexpressed en bloc, especially in severe haemoglobin subunit beta (\n                    <jats:italic>HBB<\/jats:italic>\n                    ) mutations, though higher transfusion doses attenuate these abnormalities.\n                  <\/jats:p>\n                  <jats:p>\n                    Other changes suggested impaired volume regulation, increased RBC adhesion and features not included in storage lesions, like downregulation of galectin\u20103 and septins (identified in TDT for the first time), which were modulated by genetic\u2010 and transfusion\u2010related variables. JAM\u2010A, an endothelium inflammation marker, was significantly related to heart T2*. Splenectomy correlated with oxidative, coagulative and inflammatory patterns. The moderate and severe genotype groups had similar proteomic and cell morphology profiles compared to the mildest cases. Upregulation of ferroportin, ICAM4, eryptosis and circadian rhythm\u2010related proteins further characterized severe\n                    <jats:italic>HBB<\/jats:italic>\n                    genotypes. Transfusion dose effects varied across clinical groups. Overall, the RBC membrane emerges as both a target and a sensitive reporter of the complex pathophysiology underlying TDT.\n                  <\/jats:p>","DOI":"10.1111\/bjh.70462","type":"journal-article","created":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T05:31:08Z","timestamp":1775799068000},"page":"1980-1992","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Red blood cell membrane proteome as a reporter of disease severity, transfusion impact and genetic background in transfusion\u2010dependent \u03b2\u2010thalassaemia"],"prefix":"10.1111","volume":"208","author":[{"given":"Konstantina","family":"Theocharaki","sequence":"first","affiliation":[{"name":"Department of Biology, School of Science National and Kapodistrian University of Athens (NKUA)  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7049-6131","authenticated-orcid":false,"given":"Sophia","family":"Delicou","sequence":"additional","affiliation":[{"name":"Thalassemia and Sickle Cell Unit, Expertise Center of Hemoglobinopathies and Their Complications Hippokration General Hospital  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aikaterini","family":"Ntzoura\u2010Kani","sequence":"additional","affiliation":[{"name":"Department of Biology, School of Science National and Kapodistrian University of Athens (NKUA)  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolaos","family":"Simantiris","sequence":"additional","affiliation":[{"name":"Department of Environment, Faculty of Environment Ionian University  Kerkira Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianna","family":"Politou","sequence":"additional","affiliation":[{"name":"Hematology Laboratory\u2010Blood Bank Aretaieio Hospital, School of Medicine, NKUA  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veroniki","family":"Komninaka","sequence":"additional","affiliation":[{"name":"Laboratory of Hematology Gennimatas General Hospital  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Samiotaki","sequence":"additional","affiliation":[{"name":"Institute for Bio\u2010Innovation, Biomedical Sciences Research Center \u201cAlexander Fleming\u201d  Vari Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerome","family":"Zoidakis","sequence":"additional","affiliation":[{"name":"Department of Biology, School of Science National and Kapodistrian University of Athens (NKUA)  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianna H.","family":"Antonelou","sequence":"additional","affiliation":[{"name":"Department of Biology, School of Science National and Kapodistrian University of Athens (NKUA)  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,9]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.24231"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2021011045"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2015.07.018"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/985210"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2024015232"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-12-397729"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13614"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2011.043612"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/gim.2016.173"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.104091"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-012-1629-5"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/16078454.2019.1568354"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcph.1297"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/prca.201600086"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/prca.200900073"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201300177"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcmd.2011.06.005"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/proteomes4040035"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2025017090"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-018-0082-x"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.2214\/ajr.173.6.10584785"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2018.00656"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1999.01367.x"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1507309112"},{"issue":"3","key":"e_1_2_12_26_1","first-page":"s126","article-title":"Deep\u2010coverage rhesus red blood cell proteome: a first comparison with the human and mouse red blood cell","volume":"8","author":"Pasini EM","year":"2010","journal-title":"Blood Transfus"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-9-96"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-68799-1"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2023.106048"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2022009114"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2017.00427"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2019.7753"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.11.4136"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-024-01859-x"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-04-152330"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2007.05.051"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12887-025-05741-2"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-64319-3"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2004.02073.x"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcmd.2010.02.001"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1997.d01-2070.x"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1159\/000112832"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2014.07.014"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms2540"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2019000467"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01630.x-i2"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2020007281"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25074118"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aal2022"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/83.5.555"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-022-01776-3"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1177\/0748730419836370"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232214275"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2020008563"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2025.102646"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12042"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-03-745349"},{"key":"e_1_2_12_58_1","first-page":"H1951","article-title":"Enhanced aggregability of red blood cells of beta\u2010thalassemia major patients","volume":"270","author":"Chen S","year":"1996","journal-title":"Am J Phys"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.17033"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.4084\/MJHID.2023.050"},{"key":"e_1_2_12_61_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.17897"}],"container-title":["British Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bjh.70462","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/bjh.70462","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bjh.70462","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T12:11:12Z","timestamp":1781525472000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bjh.70462"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,9]]},"references-count":60,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1111\/bjh.70462"],"URL":"https:\/\/doi.org\/10.1111\/bjh.70462","archive":["Portico"],"relation":{},"ISSN":["0007-1048","1365-2141"],"issn-type":[{"value":"0007-1048","type":"print"},{"value":"1365-2141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,9]]},"assertion":[{"value":"2026-01-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}