{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T03:40:07Z","timestamp":1792813207584,"version":"4.5.0"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670126"],"award-info":[{"award-number":["81670126"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81500104"],"award-info":[{"award-number":["81500104"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82500180"],"award-info":[{"award-number":["82500180"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Progression to moderate\u2010to\u2010severe myelofibrosis (MF) in JAK2\u2009V617F\u2010positive myeloproliferative neoplasms (MPNs) is often clinically silent, and bone marrow biopsy is invasive and unsuitable for regular monitoring. In this multicentre pilot proof\u2010of\u2010concept study, we aimed to develop an interpretable, non\u2010invasive model using peripheral blood parameters to detect significant fibrosis and to explore the inflammatory mechanisms involved. A total of 336 JAK2\u2009V617F\u2010positive MPN patients were enrolled for model training and 92 for external validation. Candidate features (blood counts, cytokines, clinical data) were screened using least absolute shrinkage and selection operator (LASSO) and Boruta algorithms. Ten machine learning methods were compared; model discrimination, calibration and clinical benefit were evaluated. Molecular validation focused on interleukin\u20101\u03b2 (IL\u20101\u03b2) in\n                    <jats:italic>Jak2V617F<\/jats:italic>\n                    knock\u2010in mice and co\u2010culture assays. The support vector machine model yielded the best discrimination (area under the curve [AUC]\u2009=\u20090.916, 95% CI 0.83\u20130.99), good calibration and highest clinical utility. SHapley Additive exPlanations (SHAP) interpretation identified haemoglobin, IL\u20101\u03b2 and age as the most influential features. Preclinical experiments confirmed IL\u20101\u03b2 upregulation with fibrosis progression, reversed by ruxolitinib or pegylated interferon\u2010\u03b1 but not hydroxyurea (hydroxycarbamide). This multicentre pilot proof\u2010of\u2010concept study demonstrates that an interpretable blood\u2010based tool shows preliminary accuracy for identifying moderate\u2010to\u2010severe MF and underscores IL\u20101\u03b2 as a mechanistic biomarker. The data support potential utility as a complementary approach; bone marrow biopsy remains the reference standard.\n                  <\/jats:p>","DOI":"10.1111\/bjh.70552","type":"journal-article","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T01:30:00Z","timestamp":1779931800000},"page":"63-74","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Interpretable machine learning model using peripheral blood for non\u2010invasive detection of moderate\u2010to\u2010severe myelofibrosis in\n                    <scp>JAK2 V617F<\/scp>\n                    \u2010positive\n                    <scp>MPNs<\/scp>\n                    : A multicentre pilot proof\u2010of\u2010concept study"],"prefix":"10.1111","volume":"209","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8166-4022","authenticated-orcid":false,"given":"Zhuanghui","family":"Hao","sequence":"first","affiliation":[{"name":"Key Laboratory of Molecular 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Hematological Diseases of Shanxi Province, Department of Hematology The Second Hospital of Shanxi Medical University  Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhua","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Basic Medicine Shanxi University of Chinese Medicine  Jinzhong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Xia","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Diagnosis and Treatment of Hematological Diseases of Shanxi Province, Department of Hematology The Second Hospital of Shanxi Medical University  Taiyuan China"},{"name":"Department of Medical Genetics Shanxi Medical University  Taiyuan Shanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Diagnosis and Treatment of Hematological Diseases of Shanxi Province, Department of Hematology The Second Hospital of Shanxi Medical University  Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xialin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Hematology The First Hospital of Shanxi Medical University  Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyi","family":"Feng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Diagnosis and Treatment of Hematological Diseases of Shanxi Province, Department of Hematology The Second Hospital of Shanxi Medical University  Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Diagnosis and Treatment of Hematological Diseases of Shanxi Province, Department of Hematology The Second Hospital of Shanxi Medical University  Taiyuan 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