{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T20:13:30Z","timestamp":1783455210562,"version":"3.55.0"},"reference-count":60,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,5,24]],"date-time":"2026-05-24T00:00:00Z","timestamp":1779580800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,24]],"date-time":"2026-05-24T00:00:00Z","timestamp":1779580800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Classical cytogenetics (CC) encompassing fluorescence in\u00a0situ hybridisation (FISH) and karyotype remains central to the diagnostic work\u2010up of non\u2010Hodgkin lymphoma (NHL). However, karyotype is limited by restricted access to fresh tumour tissue and low resolution, while FISH provides targeted information. Optical genome mapping (OGM) has emerged as a genome\u2010wide, high\u2010resolution approach capable of detecting both translocations and copy number variations in a single assay. To assess its feasibility and diagnostic performance in routine practice, we prospectively compared OGM to CC in 105 patients with NHL. OGM was technically feasible in both fresh and frozen tissue specimens. Among 511 cytogenetic abnormalities (CAs) directly compared, 91.1% were concordant between the two methods. Using the low allele fraction guided assembly pipeline, OGM identified all 77 immunoglobulin loci rearrangements, with a sensitivity threshold of 5%. Overall, OGM detected diagnostically relevant CAs in 99\/105 patients (94.2%). OGM outperformed CC in 10\/105 patients (9.5%), revealing cryptic rearrangements involving major target genes (\n                    <jats:italic>MYC<\/jats:italic>\n                    ,\n                    <jats:italic>BCL2<\/jats:italic>\n                    and\n                    <jats:italic>BCL6<\/jats:italic>\n                    ) as well as diagnostically relevant CAs in cases with uncertain diagnoses or non\u2010informative karyotypes. In conclusion, OGM demonstrated feasibility in routine practice and superior diagnostic performance compared with CC, supporting its integration into the standard genetic work\u2010up of NHL.\n                  <\/jats:p>","DOI":"10.1111\/bjh.70575","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T05:13:08Z","timestamp":1779685988000},"page":"31-41","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Optical genome mapping improves detection and characterisation of cytogenetic abnormalities in non\u2010Hodgkin lymphomas"],"prefix":"10.1111","volume":"209","author":[{"given":"Coura","family":"Fall","sequence":"first","affiliation":[{"name":"Department of Biological Hematology, Hematological Genetics Unit Grenoble Alpes University Hospital  Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4574-3663","authenticated-orcid":false,"given":"Agn\u00e8s","family":"Daudignon","sequence":"additional","affiliation":[{"name":"Institute of Medical Genetics Lille University Hospital  Lille France"},{"name":"Hematology and Immunology Laboratory Valenciennes University Hospital  Valenciennes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9verine","family":"Valmary\u2010Degano","sequence":"additional","affiliation":[{"name":"Department of Pathology Grenoble Alpes University Hospital  Grenoble France"},{"name":"INSERM U1209, CNRS UMR 5309, Institute for Advanced Biosciences (IAB) University Grenoble Alpes  Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0765-1100","authenticated-orcid":false,"given":"Julie","family":"Mondet","sequence":"additional","affiliation":[{"name":"Department of Pathology Grenoble Alpes University Hospital  Grenoble France"},{"name":"INSERM U1209, CNRS UMR 5309, Institute for Advanced Biosciences (IAB) University Grenoble Alpes  Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucile","family":"Bussot","sequence":"additional","affiliation":[{"name":"Department of Clinical Hematology Grenoble\u2010Alpes University Hospital  Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lysiane","family":"Molina","sequence":"additional","affiliation":[{"name":"Department of Clinical Hematology Grenoble\u2010Alpes University Hospital  Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Chevalier","sequence":"additional","affiliation":[{"name":"Department of Biological Hematology, Cell Hematology Unit Grenoble Alpes University Hospital  Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2935-2532","authenticated-orcid":false,"given":"Edouard","family":"Bonneville","sequence":"additional","affiliation":[{"name":"Department of Immunology, Flow Cytometry Immunophenotyping Unit Grenoble Alpes University Hospital  Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Aubert","sequence":"additional","affiliation":[{"name":"Department of Clinical Hematology Grenoble\u2010Alpes University Hospital  Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Tondeur","sequence":"additional","affiliation":[{"name":"Department of Biological Hematology, Hematological Genetics Unit Grenoble Alpes University Hospital  Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e9mentine","family":"Legrand","sequence":"additional","affiliation":[{"name":"Department of Biological Hematology, Hematological Genetics Unit Grenoble Alpes University Hospital  Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Baptiste","family":"Gaillard","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Cytogenomics, Hematological Genetics Unit Montpellier University Hospital  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4074-1623","authenticated-orcid":false,"given":"H\u00e9l\u00e8ne","family":"Guermouche","sequence":"additional","affiliation":[{"name":"Institute of Medical Genetics Lille University Hospital  Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1976-2668","authenticated-orcid":false,"given":"Anouk","family":"Emadali","sequence":"additional","affiliation":[{"name":"INSERM U1209, CNRS UMR 5309, Institute for Advanced Biosciences (IAB) University Grenoble Alpes  Grenoble France"},{"name":"Health Research and Innovation Grenoble Alpes University Hospital  Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvain","family":"Carras","sequence":"additional","affiliation":[{"name":"Department of Biological Hematology, Hematological Genetics Unit Grenoble Alpes University Hospital  Grenoble France"},{"name":"INSERM U1209, CNRS UMR 5309, Institute for Advanced Biosciences (IAB) University Grenoble Alpes  Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0424-3731","authenticated-orcid":false,"given":"Christine","family":"Lefebvre","sequence":"additional","affiliation":[{"name":"Department of Biological Hematology, Hematological Genetics Unit Grenoble Alpes University Hospital  Grenoble France"},{"name":"INSERM U1209, CNRS UMR 5309, Institute for Advanced Biosciences (IAB) University Grenoble Alpes  Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375\u2010022\u201001620\u20102"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2022015851"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102017\u201012\u2010820605"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102011\u201001\u2010330795"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2021006034"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2024024251"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22123"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.31328"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102018\u201009\u2010873083"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375\u2010022\u201001561\u2010w"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2012.070458"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.retram.2023.103425"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.retram.2023.103428"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2018.207290"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2010.09.011"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2019002600"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102018\u201007\u2010862151"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0263980"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102013\u201006\u2010507996"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2018.207928"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2022007583"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375\u2010022\u201001652\u20108"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22971"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14143376"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2025.01.003"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.26587"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2025017980"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.modpat.2025.100951"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1159\/isbn.978-3-318-06867-2"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1159\/000538512"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2018023572"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2020005289"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102010\u201003\u2010272278"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2021.06.001"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers17091436"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.70349"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1097\/HS9.0000000000000925"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.27688"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.27690"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102013\u201010\u2010535443"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2021005872"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2022007779"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591\u2010018\u20100016\u20108"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2022018639"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.17550"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102017\u201004\u2010779736"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12082120"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102015\u201002\u2010628792"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102018\u201007\u2010865428"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102015\u201005\u2010647602"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.19.00743"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102014\u201005\u2010578963"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2018.06.006"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2023022354"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22849"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41408\u2010020\u201000395\u2010y"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.27575"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20100716\u20104750\u20109_20"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2020.03.015"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13148\u2010024\u201001788\u20107"}],"container-title":["British Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bjh.70575","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/bjh.70575","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bjh.70575","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T19:14:36Z","timestamp":1783451676000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bjh.70575"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,24]]},"references-count":60,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1111\/bjh.70575"],"URL":"https:\/\/doi.org\/10.1111\/bjh.70575","archive":["Portico"],"relation":{},"ISSN":["0007-1048","1365-2141"],"issn-type":[{"value":"0007-1048","type":"print"},{"value":"1365-2141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,24]]},"assertion":[{"value":"2026-03-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}