{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T06:12:26Z","timestamp":1789193546325,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T00:00:00Z","timestamp":1783728000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T00:00:00Z","timestamp":1783728000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["55\/4\/10\/CARE\u2010AML\/2018\u2010NCD\u2010II"],"award-info":[{"award-number":["55\/4\/10\/CARE\u2010AML\/2018\u2010NCD\u2010II"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Leukaemic stem cells (LSCs) are chemo\u2010resistant leukaemia\u2010initiating clones that drive refractoriness and relapse in acute myeloid leukaemia (AML). The study evaluated the prognostic significance of LSC burden in treatment na\u00efve AML patients. LSCs were quantified using Next\u2010generation flow cytometry; patients were stratified into LSC\n                    <jats:sup>neg\/low<\/jats:sup>\n                    and LSC\n                    <jats:sup>high<\/jats:sup>\n                    groups based on the median LSC percentage, and correlated with post\u2010induction morphological remission and measurable residual disease (MRD) status, and long\u2010term outcomes including relapse\u2010free survival (RFS), event\u2010free survival (EFS) and overall survival (OS). LSCs were detectable in 93.5% patients, with a median frequency of 0.386%. Patients with LSC\n                    <jats:sup>neg\/low<\/jats:sup>\n                    (\u22640.386%) had higher rates of complete remission (CR) (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.05) and MRD negativity (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.002). Lower LSC burden was associated with improved RFS, EFS and OS (all\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) suggesting the prognostic relevance of LSC quantitation in AML. In both Cluster of Differentiation 34 positve (CD34+) and CD34 negative (CD34\u2212) AML, low LSC% was associated with superior survival outcomes (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). LSC burden is a strong, independent predictor of response and survival in AML, and its integration with European LeukemiaNet risk\u2010stratification and MRD assessment may enhance risk refinement and individualized therapy.\n                  <\/jats:p>","DOI":"10.1111\/bjh.70679","type":"journal-article","created":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T07:55:15Z","timestamp":1783756515000},"page":"1119-1127","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Leukaemic stem cell burden enhances prognostic precision in the non\u2010adverse European LeukemiaNet risk group of acute myeloid leukaemia"],"prefix":"10.1111","volume":"209","author":[{"given":"Aafreen","family":"Khan","sequence":"first","affiliation":[{"name":"Laboratory Oncology Unit Dr. BRAIRCH, AIIMS  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1759-7857","authenticated-orcid":false,"given":"Smeeta","family":"Gajendra","sequence":"additional","affiliation":[{"name":"Laboratory Oncology Unit Dr. BRAIRCH, AIIMS  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1858-6081","authenticated-orcid":false,"given":"Sandeep","family":"Rai","sequence":"additional","affiliation":[{"name":"Laboratory Oncology Unit Dr. BRAIRCH, AIIMS  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepshi","family":"Thakral","sequence":"additional","affiliation":[{"name":"Laboratory Oncology Unit Dr. BRAIRCH, AIIMS  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2655-2850","authenticated-orcid":false,"given":"Vivek Kumar","family":"Singh","sequence":"additional","affiliation":[{"name":"Laboratory Oncology Unit Dr. BRAIRCH, AIIMS  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashish Datt","family":"Upadhyay","sequence":"additional","affiliation":[{"name":"Clinical Research Unit AIIMS  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sameer","family":"Bakhshi","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology Dr.\u00a0BRAIRCH, AIIMS  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7746-547X","authenticated-orcid":false,"given":"Rachna","family":"Seth","sequence":"additional","affiliation":[{"name":"Department of Paediatrics AIIMS  New\u00a0Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranjit Kumar","family":"Sahoo","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology Dr.\u00a0BRAIRCH, AIIMS  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ritu","family":"Gupta","sequence":"additional","affiliation":[{"name":"Laboratory Oncology Unit Dr. BRAIRCH, AIIMS  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,11]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.6004\/jnccn.2023.0025"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.14007"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2015.0617"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/10428194.2020.1802450"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(18)31041\u20109"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.12750"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13133170"},{"issue":"16","key":"e_1_2_14_9_1","first-page":"4403","article-title":"Human acute myeloid leukemia CD34+\/CD38\u2212 progenitor cells have decreased sensitivity to chemotherapy and Fas\u2010induced apoptosis, reduced immunogenicity, and impaired dendritic cell transformation capacities1","volume":"60","author":"Costello RT","year":"2000","journal-title":"Cancer Res"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1350"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/367645a0"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI41495"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2023.101108"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102016\u201010\u2010696054"},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102009\u201002\u2010206565"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0107587"},{"key":"e_1_2_14_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2022019160"},{"key":"e_1_2_14_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/10428194.2021.2005042"},{"key":"e_1_2_14_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2022016867"},{"key":"e_1_2_14_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers17091405"},{"key":"e_1_2_14_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375\u2010022\u201001613\u20101"},{"key":"e_1_2_14_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41408\u2010022\u201000726\u20101"},{"key":"e_1_2_14_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2023.1109518"},{"key":"e_1_2_14_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.14250"},{"key":"e_1_2_14_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2026.108170"},{"key":"e_1_2_14_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clml.2020.05.004"},{"key":"e_1_2_14_27_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102009\u201008\u2010238899"},{"key":"e_1_2_14_28_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20151775"},{"key":"e_1_2_14_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277\u2010012\u20101501\u20107"},{"key":"e_1_2_14_30_1","unstructured":"ZahranAM AlySS RayanA El\u2010BadawyO FattahMA AliAM et\u00a0al.Survival outcomes of CD34 + CD38 \u2212 LSCs and their expression of CD123 in adult AML patients[Internet]. Available from:www.oncotarget.com"},{"key":"e_1_2_14_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2009.04.018"},{"key":"e_1_2_14_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clml.2021.03.009"},{"key":"e_1_2_14_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2022.867684"},{"key":"e_1_2_14_34_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078\u20100432.CCR\u201005\u20100468"},{"key":"e_1_2_14_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2009.10.001"},{"key":"e_1_2_14_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12051174"},{"key":"e_1_2_14_37_1","doi-asserted-by":"publisher","DOI":"10.3109\/08880018.2010.528171"},{"key":"e_1_2_14_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clml.2019.11.018"},{"key":"e_1_2_14_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375\u2010025\u201002539\u20100"},{"key":"e_1_2_14_40_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102005\u201006\u20102325"},{"key":"e_1_2_14_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375\u2010018\u20100180\u20103"}],"container-title":["British Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bjh.70679","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/bjh.70679","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bjh.70679","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T06:03:38Z","timestamp":1789193018000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bjh.70679"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,11]]},"references-count":40,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/bjh.70679"],"URL":"https:\/\/doi.org\/10.1111\/bjh.70679","archive":["Portico"],"relation":{},"ISSN":["0007-1048","1365-2141"],"issn-type":[{"value":"0007-1048","type":"print"},{"value":"1365-2141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,11]]},"assertion":[{"value":"2026-01-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}