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Mucosal\u2010associated invariant T (MAIT) cells are mucosa\u2010enriched unconventional T cells with major histocompatibility complex class I\u2010related protein 1 (MR1)\u2010restricted, major histocompatibility complex (MHC)\u2010independent recognition, suggesting a potentially reduced risk of alloreactivity. Our previous work showed that higher graft MAIT\u2010cell levels were associated with improved post\u2010transplant MAIT\u2010cell reconstitution and a lower incidence of GI aGVHD. Single\u2010cell ribonucleic acid (RNA) sequencing (sc\u2010RNA\u2010seq) and murine models revealed their functional heterogeneity in immune regulation, tissue repair and chemotaxis\u2014supporting their role as both biomarkers and therapeutic targets. In this prospective study, spectral flow cytometry was used to characterize MAIT\u2010cell phenotypes in peripheral blood stem cell grafts. higher graft MAIT\u2010cell abundance was associated with more robust early post\u2010transplant MAIT\u2010cell reconstitution and a lower risk of GI aGVHD. A three\u2010marker predictive panel based on MAIT\u2010cell functional markers (C\u2010C chemokine receptor type 2 [CCR2], interleukin\u20104 [IL\u20104], interleukin\u201017A [IL\u201017A]) achieved an area under the receiver operating characteristic curve (AUC) of 0.80, increasing to 0.85 after adjustment for clinical covariates. These findings identify graft\u2010derived MAIT cells as a predictive immune\u2010associated biomarker for GI aGVHD, enabling pre\u2010transplant risk stratification and supporting precision prevention strategies. Trial registration: ChiCTR2500095349.<\/jats:p>","DOI":"10.1111\/bjh.70683","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T03:29:37Z","timestamp":1784172577000},"page":"1208-1219","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Immune features of graft\u2010derived mucosal\u2010associated invariant T cells predict gastrointestinal graft\u2010versus\u2010host disease"],"prefix":"10.1111","volume":"209","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2329-5625","authenticated-orcid":false,"given":"Gao","family":"Mengge","sequence":"first","affiliation":[{"name":"Peking University People's Hospital, Peking University Institute of Hematology, National Clinical Research Center for Hematologic Disease, Beijing Key Laboratory of Cell and Gene Therapy for Hematologic Malignancies Peking University  Beijing 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Diagnosis and Treatments of Hematologic Malignancies Chinese Academy of Medical Sciences, 2019RU029  Beijing China"},{"name":"Peking\u2010Tsinghua Center for Life Sciences  Beijing China"},{"name":"Collaborative Innovation Center of Hematology Peking University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7749-8087","authenticated-orcid":false,"given":"Zhao","family":"Xiaosu","sequence":"additional","affiliation":[{"name":"Peking University People's Hospital, Peking University Institute of Hematology, National Clinical Research Center for Hematologic Disease, Beijing Key Laboratory of Cell and Gene Therapy for Hematologic Malignancies Peking University  Beijing China"},{"name":"Research Unit of Key Technique for Diagnosis and Treatments of Hematologic Malignancies Chinese Academy of Medical Sciences, 2019RU029  Beijing China"},{"name":"Collaborative Innovation Center of Hematology Peking University  Beijing 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