{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T06:13:49Z","timestamp":1789193629076,"version":"build-2803163510"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Immune thrombocytopenia (ITP) is an autoimmune disorder characterized by platelet destruction and dysfunction associated with anti\u2010platelet antibodies. This study evaluated the relationship between total anti\u2010platelet antibody burden, measured using a modified platelet immunofluorescence test (PIFT), platelet functional responses and clinical features in chronic ITP. Thirty\u2010one patients with chronic ITP and 20 healthy controls were included. Bleeding severity was assessed, and platelet function was analysed in peripheral blood by measuring P\u2010selectin expression, PAC\u20101 binding (antibody against active conformation of GPIIb\/IIIa)\u00a0and reactive oxygen species (ROS) generation at baseline and following agonist stimulation. Relative platelet\u2010reactive antibody burden was assessed using a ratio\u2010based PIFT assay. ITP patients demonstrated significantly higher antibody burden compared with controls. Increased platelet\u2010reactive immunoglobulin G (IgG) signals were associated with reduced platelet responsiveness to agonist stimulation. Antibody burden correlated with bleeding severity (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01) but not with platelet count. Non\u2010responders exhibited significantly higher antibody levels than responders. receiver operator characteristic (ROC) analysis demonstrated discrimination between responder groups at a PIFT cut\u2010off \u22653.85 (area under the curve [AUC] 0.877, sensitivity 80%; specificity 87%). Patients above this threshold showed attenuated platelet functional responses. Taken together, this study concluded that quantitative assessment of total antibody burden against platelets using modified PIFT is associated with platelet dysfunction, bleeding severity and treatment response status in chronic ITP.\n                  <\/jats:p>","DOI":"10.1111\/bjh.70706","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T10:54:12Z","timestamp":1786445652000},"page":"1258-1268","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Design of a modified platelet immunofluorescence test to assess platelet\u2010reactive antibody burden and its association with platelet functional exhaustion and clinical features in chronic immune thrombocytopenia"],"prefix":"10.1111","volume":"209","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6807-0908","authenticated-orcid":false,"given":"Ehteramolsadat","family":"Hosseini","sequence":"first","affiliation":[{"name":"Blood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0445-3192","authenticated-orcid":false,"given":"Nazanin","family":"Heidari","sequence":"additional","affiliation":[{"name":"Blood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2775-2347","authenticated-orcid":false,"given":"Mohammad","family":"Faranoush","sequence":"additional","affiliation":[{"name":"Pediatric Growth and Development Research Center, Institute of Endocrinology and Metabolism Iran University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8247-5282","authenticated-orcid":false,"given":"Seyed Mohammad 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