{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T13:51:08Z","timestamp":1792158668661,"version":"4.4.0"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Measurable residual disease (MRD) monitoring is crucial for prognostic stratification in paediatric acute myeloid leukaemia (AML). This study compared the performance of multiparameter flow cytometry (MFC) and droplet digital polymerase chain reaction (ddPCR) for MRD assessment in 116 paediatric AML patients at end of induction (EOI)\u20101, EOI\u20102 and end of consolidation (EOC)\u20101. Compared to MFC, ddPCR revealed a significantly slower rate of MRD negativity conversion, with moderate concordance between methods. Both MFC and ddPCR were significant predictors of 5\u2010year event\u2010free survival (EFS) at EOIs. For instance, MFC\u2010MRD \u22651% at EOI\u20101 was associated with a 5\u2010year EFS of 50.2\u2009\u00b1\u200919.8% vs. 79.4\u2009\u00b1\u20096.4% for patients with MRD &lt;1% (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.003), while corresponding ddPCR rates were 41.0\u2009\u00b1\u200923.8% vs. 91.7\u2009\u00b1\u20094.5% (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). Notably, the prognostic power of MFC diminished at EOC\u20101, while ddPCR remained significant. Subgroup analysis by genetic alterations revealed that ddPCR provided significant prognostic stratification across all genetic subgroups while MFC did not. Combining both methods yielded improved risk discrimination. Patients with double positive MRD presented very low survival rates either at EOI\u20101, EOI\u20102 and EOC\u20101. In conclusion, ddPCR offers higher sensitivity and sustained prognostic performance, whereas MFC remains a practical front\u2010line tool; integrating the two methods enables more precise risk stratification.\n                  <\/jats:p>","DOI":"10.1111\/bjh.70714","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T05:32:33Z","timestamp":1786512753000},"page":"1562-1573","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multiparameter flow cytometry versus droplet digital polymerase chain reaction for monitoring measurable residual disease in paediatric fusion gene\u2010positive acute myeloid leukaemia"],"prefix":"10.1111","volume":"209","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-7087-3143","authenticated-orcid":false,"given":"Rongrong","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Hematology\/Oncology National Clinical Research Center for Children and Adolescents' Health and Diseases, Children's Hospital, Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Hematology\/Oncology National Clinical Research Center for Children and Adolescents' Health and Diseases, Children's Hospital, Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Hematology\/Oncology National Clinical Research Center for Children and Adolescents' Health and Diseases, Children's Hospital, Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiqun","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Hematology\/Oncology National Clinical Research Center for Children and Adolescents' Health and Diseases, Children's Hospital, Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fenying","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Hematology\/Oncology National Clinical Research Center for Children and Adolescents' Health and Diseases, Children's Hospital, Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Hematology\/Oncology National Clinical Research Center for Children and Adolescents' Health and Diseases, Children's Hospital, Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diying","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Hematology\/Oncology National Clinical Research Center for Children and Adolescents' Health and Diseases, Children's Hospital, Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Hematology\/Oncology National Clinical Research Center for Children and Adolescents' Health and Diseases, Children's Hospital, Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiqi","family":"Tang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Infectious Diseases The First Affiliated Hospital, Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1409-5517","authenticated-orcid":false,"given":"Yongmin","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Hematology\/Oncology 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