{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T09:53:33Z","timestamp":1791453213980,"version":"4.3.3"},"reference-count":18,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100012538","name":"Swedish Cancer Foundation","doi-asserted-by":"publisher","award":["24 3277 Pj"],"award-info":[{"award-number":["24 3277 Pj"]}],"id":[{"id":"10.13039\/100012538","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["2021\u201002826_VR"],"award-info":[{"award-number":["2021\u201002826_VR"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>Autoimmune neutropenia (AIN) in early childhood is caused by autoantibodies targeting neutrophil surface antigens, most commonly CD16 (FCGR3B), and must be distinguished from congenital neutropenia, malignancy\u2010associated neutropenia and chronic idiopathic neutropenia (CIN). In this study, the diagnostic performance of the cell\u2010based granulocyte agglutination test (GAT) and granulocyte immunofluorescence test (GIFT) was compared with the bead\u2010based LabScreen Multi (LSM) assay in 214\u2010suspected AIN cases. While 36% of samples were positive for anti\u2010CD16 antibodies in GAT or GIFT, only 19% reacted against the same antigen in LSM, primarily missing weak or Immunoglobulin M (IgM)\u2010restricted antibody responses. [Correction added on 8 October 2026, after first online publication: The preceding sentence was corrected]. Patients testing positive in all three assays exhibited significantly higher pro\u2010LL\u201037 levels and lower absolute neutrophil counts (ANCs), indicating preserved granulopoiesis with peripheral destruction. Our findings question the use of LSM as a standalone test in AIN diagnostics. Incorporating pro\u2010LL\u201037 and ANC in AIN diagnosis may improve differentiation between antibody\u2010mediated neutropenia and other neutropenia forms in children.<\/jats:p>","DOI":"10.1111\/bjh.70783","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T12:38:48Z","timestamp":1787747928000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Autoimmune neutropenia in children: Test cells versus beads and a role for pro\u2010\n                    <scp>LL<\/scp>\n                    \u201037 and absolute neutrophil count"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9423-5921","authenticated-orcid":false,"given":"Paul","family":"Ratcliffe","sequence":"first","affiliation":[{"name":"Center for Haematology and Regenerative Medicine (HERM), Department of Medicine Huddinge, Karolinska Institute, and Clinical Immunology and Transfusion Medicine Karolinska University Hospital  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1806-0768","authenticated-orcid":false,"given":"Alexandra Haara","family":"L\u00f6fstedt","sequence":"additional","affiliation":[{"name":"Unit for Paediatrics, Department of Clinical Intervention and Technology, Karolinska Institute, and Paediatric Haematology Karolinska University Hospital  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9871-0961","authenticated-orcid":false,"given":"Mikael","family":"Sundin","sequence":"additional","affiliation":[{"name":"Unit for Paediatrics, Department of Clinical Intervention and Technology, Karolinska Institute, and Paediatric Haematology Karolinska University Hospital  Stockholm 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