{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T03:27:38Z","timestamp":1771126058797,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,7,2]],"date-time":"2013-07-02T00:00:00Z","timestamp":1372723200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2013,10]]},"abstract":"\n Objective<\/jats:title>\n \n \n \n \n To investigate whether secretion of human \u03b2\u2010defensin 2 (\n HBD<\/jats:styled-content>\n \u20102) is induced by bacillus\n C<\/jats:styled-content>\n almette\u2010\n G<\/jats:styled-content>\n u\u00e9rin (\n BCG<\/jats:styled-content>\n ) and to determine whether\n HBD<\/jats:styled-content>\n \u20102 affects\n BCG<\/jats:styled-content>\n internalisation in bladder cancer cells.\n <\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n \n \n \n \n Reverse transcription\u2010polymerase chain reaction analysis was used to determine whether\n HBD<\/jats:styled-content>\n \u20102\n mRNA<\/jats:styled-content>\n increases after incubation with\n BCG<\/jats:styled-content>\n .\n HBD<\/jats:styled-content>\n \u20102 proteins in 5637 and\n T<\/jats:styled-content>\n 24 human bladder cancer cell lines were assayed by enzyme\u2010linked immunosorbent assay.\n <\/jats:p>\n <\/jats:list-item>\n \n \n The internalisation rate was evaluated by double immunofluorescence assay and confocal microscopy to test the optimal dose of\n HBD<\/jats:styled-content>\n \u20102 for\n BCG<\/jats:styled-content>\n internalisation.\n <\/jats:p>\n <\/jats:list-item>\n \n \n We also investigated the difference in internalisation rates and cell viability between recombinant\n HBD<\/jats:styled-content>\n \u20102 protein, anti\u2010\n HBD<\/jats:styled-content>\n \u20102 antibody, and\n HBD<\/jats:styled-content>\n \u20102 plus anti\u2010\n HBD<\/jats:styled-content>\n \u20102 antibody pretreatments.\n <\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n \n \n \n BCG<\/jats:styled-content>\n induced\n HBD<\/jats:styled-content>\n \u20102\n mRNA<\/jats:styled-content>\n expression and\n HBD<\/jats:styled-content>\n \u20102 production dose and time\u2010dependently in bladder cancer cells and affected\n BCG<\/jats:styled-content>\n internalisation.\n <\/jats:p>\n <\/jats:list-item>\n \n \n Pretreatment with recombinant\n HBD<\/jats:styled-content>\n \u20102 protein lowered internalisation of\n BCG<\/jats:styled-content>\n dose\u2010dependently. Moreover, anti\u2010\n HBD<\/jats:styled-content>\n \u20102 antibody prevented the effect of\n HBD<\/jats:styled-content>\n \u20102 on\n BCG<\/jats:styled-content>\n internalisation in bladder cancer cells.\n <\/jats:p>\n <\/jats:list-item>\n \n \n The internalisation rate of\n BCG<\/jats:styled-content>\n pretreated with anti\u2010\n HBD<\/jats:styled-content>\n \u20102 antibody was higher than that in the control in 5637 (\n P<\/jats:italic>\n < 0.01) and T24 cells (\n P<\/jats:italic>\n < 0.05). The\n BCG<\/jats:styled-content>\n internalisation rate in cells pretreated with anti\u2010\n HBD<\/jats:styled-content>\n \u20102 antibody plus recombinant\n HBD<\/jats:styled-content>\n \u20102 protein was higher than that in the control in 5637 (\n P<\/jats:italic>\n < 0.01) and T24 cells (\n P<\/jats:italic>\n < 0.05).\n <\/jats:p>\n <\/jats:list-item>\n \n \n \n M<\/jats:styled-content>\n ycobacterium bovis\n <\/jats:italic>\n \u2005\n BCG<\/jats:styled-content>\n decreased bladder cancer cell viability, and anti\u2010\n HBD<\/jats:styled-content>\n \u20102 antibody prevented the inhibitory role of\n HBD<\/jats:styled-content>\n \u20102 on the anti\u2010proliferative effects of M.\n bovis<\/jats:italic>\n \u2005\n BCG<\/jats:styled-content>\n in bladder cancer cells\n <\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n \n \n \n Bladder cancer cells produce\n HBD<\/jats:styled-content>\n \u20102 when they are infected by\n BCG<\/jats:styled-content>\n to defend themselves against\n BCG<\/jats:styled-content>\n internalisation, which plays an important role during the initiation and propagation of the immunotherapeutic response in bladder cancer cells.\n <\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/bju.12196","type":"journal-article","created":{"date-parts":[[2013,7,2]],"date-time":"2013-07-02T10:13:39Z","timestamp":1372760019000},"page":"781-790","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Human \u03b2\u2010defensin 2 may inhibit internalisation of bacillus\n C<\/scp>\n almette\u2010\n G<\/scp>\n u\u00e9rin (\n BCG<\/scp>\n ) in bladder cancer cells"],"prefix":"10.1111","volume":"112","author":[{"given":"Jung Hoon","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Urology Chung\u2010Ang University College of Medicine Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soon\u2010Ja","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Urology Chung\u2010Ang University College of Medicine Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyung Mee","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Urology Chung\u2010Ang University College of Medicine Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"In Ho","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Urology Chung\u2010Ang University College of Medicine Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,7,2]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"280","article-title":"Intravesical therapy for superficial bladder cancer","volume":"18","author":"Malkowicz SB","year":"2000","journal-title":"Semin Urol Oncol"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.2217\/1750743X.1.2.281"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200636379"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-6-116"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI1861"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.1999.5.265"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2006.04.001"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19961201)25:4<381::AID-CYTO10>3.0.CO;2-R"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2006.08.009"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.119.4.1131"},{"key":"e_1_2_8_12_1","doi-asserted-by":"crossref","unstructured":"AliAS LanzM TownesCLet\u2009al.Release of the anti\u2010microbial peptide beta\u2010defensin 2 protects against attack by flagellatedEscherichia coliin human urothelium. 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