{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T14:15:57Z","timestamp":1761747357192,"version":"build-2065373602"},"reference-count":14,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,4,9]],"date-time":"2014-04-09T00:00:00Z","timestamp":1397001600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2014,5]]},"abstract":"\n Objective<\/jats:title>\n \n \n \n \n To describe a microsurgical technique for denervation of the spermatic cord and use of multiphoton microscopy (\n MPM<\/jats:styled-content>\n ) laser to identify and ablate residual nerves after microsurgical denervation.\n <\/jats:p>\n <\/jats:list-item>\n \n To evaluate structural and functional changes in the rat testis and vas deferens after denervation.<\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n \n \n \n \n Nine\n S<\/jats:styled-content>\n prague\u2010\n D<\/jats:styled-content>\n awley rats were divided into three experimental groups: sham, microsurgical denervation of the spermatic cord (\n MDSC<\/jats:styled-content>\n ), and\n MDSC<\/jats:styled-content>\n immediately followed by laser ablation with\n MPM<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:list-item>\n \n \n At 2 months after surgery, we assessed testicular volume, functional circulation of the testicular artery with\n D<\/jats:styled-content>\n oppler, patency of the vas deferens, and histology of the testis and vas deferens.\n <\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n \n \n \n There was a significant decrease in the median number of nerves remaining around the vas deferens with\n MDSC<\/jats:styled-content>\n alone (3.5 nerves) or\n MDSC<\/jats:styled-content>\n with\n MPM<\/jats:styled-content>\n (1.5 nerves) compared with sham rats (15.5 nerves) (\n P<\/jats:italic>\n = 0.003).\n <\/jats:p>\n <\/jats:list-item>\n \n \n Although,\n MDSC<\/jats:styled-content>\n with\n MPM<\/jats:styled-content>\n resulted in the fewest remaining nerves, this result was similar to\n MDSC<\/jats:styled-content>\n alone (\n P<\/jats:italic>\n = 0.29).\n <\/jats:p>\n <\/jats:list-item>\n \n No deleterious effects on spermatogenesis or vas patency were seen in the experimental groups when compared with the sham rats.<\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n \n \n A microsurgical approach can be used to effectively and safely denervate the rat spermatic cord with minimal changes to structure and function of the testis and vas deferens.<\/jats:p>\n <\/jats:list-item>\n \n \n MPM<\/jats:styled-content>\n can be used as an adjunct to identify and ablate residual nerves after\n MDSC<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/bju.12421","type":"journal-article","created":{"date-parts":[[2013,8,23]],"date-time":"2013-08-23T01:19:15Z","timestamp":1377220755000},"page":"795-800","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Microsurgical denervation of rat spermatic cord: safety and efficacy data"],"prefix":"10.1111","volume":"113","author":[{"given":"Melissa A.","family":"Laudano","sequence":"first","affiliation":[{"name":"Department of Urology Weill Cornell Medical College New York Presbyterian Hospital New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. Charles","family":"Osterberg","sequence":"additional","affiliation":[{"name":"Department of Urology Weill Cornell Medical College New York Presbyterian Hospital New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seema","family":"Sheth","sequence":"additional","affiliation":[{"name":"Department of Urology Weill Cornell Medical College New York Presbyterian Hospital New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ranjith","family":"Ramasamy","sequence":"additional","affiliation":[{"name":"Department of Urology Weill Cornell Medical College New York Presbyterian Hospital New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joshua","family":"Sterling","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Weill Cornell Medical College New York Presbyterian Hospital New York NY 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