{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T20:06:01Z","timestamp":1787169961075,"version":"build-2736575974"},"reference-count":27,"publisher":"Wiley","issue":"6b","license":[{"start":{"date-parts":[[2014,4,29]],"date-time":"2014-04-29T00:00:00Z","timestamp":1398729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2014,12]]},"abstract":"\n Objective<\/jats:title>\n \n To evaluate the variability in prostate cancer gene 3 (\n PCA3<\/jats:styled-content>\n ) score over time in men with elevated serum prostate\u2010specific antigen (\n PSA<\/jats:styled-content>\n ) levels who are undergoing first or repeat prostate biopsy.\n <\/jats:p>\n <\/jats:sec>\n \n Patients and Methods<\/jats:title>\n \n A total of 360 men from two Italian institutions who had undergone at least two\n PCA3<\/jats:styled-content>\n assessments were selected. Of these, 97.5% were scheduled for first or repeat prostate biopsy because of elevated\n PSA<\/jats:styled-content>\n level and\/or positive digital rectal examination (\n DRE<\/jats:styled-content>\n ). We compared the\n PCA3<\/jats:styled-content>\n scores in men with a negative biopsy (normal parenchyma, benign prostatic hyperplasia [\n BPH<\/jats:styled-content>\n ], chronic prostatitis, high\u2010grade prostate intraepithelial neoplasia [\n HG\u2010PIN<\/jats:styled-content>\n ]) with those in men with a positive biopsy. We evaluated\n PCA3<\/jats:styled-content>\n repeated measures biological variability and its possible association with basic patient characteristics (age, family history of prostate cancer,\n DRE<\/jats:styled-content>\n , prostate volume,\n BPH<\/jats:styled-content>\n , prostatitis and\n HG\u2010PIN<\/jats:styled-content>\n ). Three different thresholds were used to evaluate the possible changes in risk class: the standard threshold (a\n PCA3<\/jats:styled-content>\n score of 35), a\n US F<\/jats:styled-content>\n ood and\n D<\/jats:styled-content>\n rug\n A<\/jats:styled-content>\n dministation\u2010approved\n PCA3<\/jats:styled-content>\n threshold of 25 and a threshold selected based on our previous research which was a\n PCA3<\/jats:styled-content>\n score of 50.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The\n PCA3<\/jats:styled-content>\n scores varied significantly (\n P<\/jats:italic>\n < 0.001) when comparing men with a negative biopsy with those with a positive biopsy (median [range]\n PCA3<\/jats:styled-content>\n score: 25 [2\u2013276] vs 43 [7\u2013331]). There was no significant difference in\n PCA3<\/jats:styled-content>\n scores in men with chronic prostatitis and\n HG\u2010PIN<\/jats:styled-content>\n compared with other men with negative biopsies. The median (range) time between the two\n PCA3<\/jats:styled-content>\n assessments was 16.2 (3\u201353.7) months. No association was found between\n PCA3<\/jats:styled-content>\n repeated measures modifications and age, family history of prostate cancer,\n DRE<\/jats:styled-content>\n ,\n BPH<\/jats:styled-content>\n , prostatitis,\n HG\u2010PIN<\/jats:styled-content>\n and use of 5\u2010\u03b1\u2010reductase inhibitors. The variability of\n PCA3<\/jats:styled-content>\n scores on repeated measures confirmed the risk class for about 80% of patients; of the remaining 20% of patients, the risk class was upgraded in two thirds and downgraded in one third.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n PCA3<\/jats:styled-content>\n score can be considered a stable marker over time in most cases but there is a group of men among whom there is clinically notable risk class change. Further investigation is required to determine the genesis of this phenomenon.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/bju.12654","type":"journal-article","created":{"date-parts":[[2014,1,29]],"date-time":"2014-01-29T00:08:47Z","timestamp":1390954127000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Fluctuation in prostate cancer gene 3 (\n PCA3<\/scp>\n ) score in men undergoing first or repeat prostate biopsies"],"prefix":"10.1111","volume":"114","author":[{"given":"Stefano","family":"De Luca","sequence":"first","affiliation":[{"name":"Department of Urology San Luigi Gonzaga Hospital Orbassano Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Passera","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine San Giovanni Battista Hospital Torino Italy"},{"name":"University of Torino Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanna","family":"Cappia","sequence":"additional","affiliation":[{"name":"Department of Pathology San Luigi Gonzaga Hospital Orbassano Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrico","family":"Bollito","sequence":"additional","affiliation":[{"name":"Department of Pathology San Luigi Gonzaga Hospital Orbassano Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donato Franco","family":"Randone","sequence":"additional","affiliation":[{"name":"Department of Urology Gradenigo Hospital Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Milillo","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Gradenigo Hospital Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro","family":"Papotti","sequence":"additional","affiliation":[{"name":"Department of Pathology San Luigi Gonzaga Hospital Orbassano Italy"},{"name":"University of Torino Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Porpiglia","sequence":"additional","affiliation":[{"name":"Department of Urology San Luigi Gonzaga Hospital Orbassano Italy"},{"name":"University of Torino Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,4,29]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2009.07.093"},{"key":"e_1_2_6_3_1","first-page":"695","article-title":"DD3(PCA3), a very sensitive and specific marker to detect prostate tumors","volume":"62","author":"Kok JB","year":"2002","journal-title":"Cancer Res"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(03)00759-3"},{"key":"e_1_2_6_5_1","first-page":"5975","article-title":"A new prostate\u2010specific gene, highly over expressed in prostate cancer","volume":"59","author":"Bussemakers MJ","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2008.06.043"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2010.09.035"},{"key":"e_1_2_6_8_1","first-page":"243","volume-title":"UICC TNM Classification of Malignant Tumors","author":"Sobin LH","year":"2009"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.pas.0000173646.99337.b1"},{"key":"e_1_2_6_10_1","first-page":"96","article-title":"Prostate cancer gene 3 urine assay cutoff in diagnosis of prostate cancer. A validation study on an italian population undergoing first and repeat biopsy","volume":"34","author":"Bollito E","year":"2012","journal-title":"Anal Quant Cytol Histol"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2010.10.039"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.6004\/jnccn.2010.0010"},{"key":"e_1_2_6_13_1","doi-asserted-by":"crossref","first-page":"1452","DOI":"10.1001\/jama.1997.03540420048028","article-title":"Prostate cancer detection in men with serum PSA concentrations of 2.6 to 4.0\u2009ng\/mL and benign prostate examination. Enhancement of specificity with free PSA measurements","volume":"277","author":"Catalona WJ","year":"1997","journal-title":"JAMA"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0302-2838(03)00201-X"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2012.11645.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2007.11.038"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2004.06.004"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2012.04.143"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.24447"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.21457"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2012.04.017"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2011.04007.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800053"},{"key":"e_1_2_6_24_1","first-page":"1","article-title":"Inflammation and preneoplastic lesions in bening prostate as risk factors for prostate cancer","volume":"41","author":"Kryvenko ON","year":"2012","journal-title":"Modern Pathol"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)00337-X"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00345-010-0580-0"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2008.06.071"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2005.063289"}],"container-title":["BJU International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbju.12654","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbju.12654","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bjui-journals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bju.12654","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T16:18:51Z","timestamp":1761754731000},"score":1,"resource":{"primary":{"URL":"https:\/\/bjui-journals.onlinelibrary.wiley.com\/doi\/10.1111\/bju.12654"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4,29]]},"references-count":27,"journal-issue":{"issue":"6b","published-print":{"date-parts":[[2014,12]]}},"alternative-id":["10.1111\/bju.12654"],"URL":"https:\/\/doi.org\/10.1111\/bju.12654","archive":["Portico"],"relation":{},"ISSN":["1464-4096","1464-410X"],"issn-type":[{"value":"1464-4096","type":"print"},{"value":"1464-410X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,4,29]]},"assertion":[{"value":"2014-04-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}