{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T19:22:22Z","timestamp":1761765742934,"version":"build-2065373602"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,8,25]],"date-time":"2014-08-25T00:00:00Z","timestamp":1408924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2014,9]]},"abstract":"\n Objective<\/jats:title>\n \n To investigate the expression of \u2018\n T<\/jats:styled-content>\n \u2010type\u2019 and \u2018\n L<\/jats:styled-content>\n \u2010type\u2019 voltage\u2010operated\n \n \n Ca\n 2<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n +<\/jats:sup>\n channels in single interstitial cells of the guinea\u2010pig prostate.\n <\/jats:p>\n <\/jats:sec>\n \n Material and Methods<\/jats:title>\n \n Whole\u2010cell and perforated patch\u2010clamp techniques were applied to prostatic interstitial cells (\n PIC<\/jats:styled-content>\n s) dispersed using collagenase.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n In contrast to prostatic myocytes,\n PIC<\/jats:styled-content>\n s under voltage clamp and filled with\n \n K<\/jats:roman>\n <\/jats:styled-content>\n +<\/jats:sup>\n (130\u2009m\n m<\/jats:sc>\n ) were distinguished by the absence of a voltage\u2010operated transient outward\n \n K<\/jats:roman>\n <\/jats:styled-content>\n +<\/jats:sup>\n current or spike discharge upon membrane depolarisation when under current clamp. Depolarisation of\n C<\/jats:styled-content>\n s\n +<\/jats:sup>\n \u2010filled\n PIC<\/jats:styled-content>\n s evoked an inward current at potentials positive to \u221260 mV, which peaked in amplitude near 0 mV. This inward current increased when\n \n B<\/jats:roman>\n <\/jats:styled-content>\n a\n 2+<\/jats:sup>\n (5\u2009m\n m<\/jats:sc>\n ) replaced the external\n \n \n Ca\n 2<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n +<\/jats:sup>\n (1.5\u2009m\n m<\/jats:sc>\n ) and displayed a variable sensitivity to the inhibitory actions of conditioning depolarisations to \u221240 mV applied before the test depolarisation or to 1\u2009\u03bc\n m<\/jats:sc>\n nifedipine, the \u2018\n L<\/jats:styled-content>\n \u2010type\u2019\n \n \n Ca\n 2<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n +<\/jats:sup>\n channel blocker. A residual inward current recorded in nifedipine was blocked by 10\u2009\u03bc\n m<\/jats:sc>\n \n \n Ni\n 2<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n +<\/jats:sup>\n .\n \n Cs<\/jats:roman>\n <\/jats:styled-content>\n +<\/jats:sup>\n \u2010filled\n PICs<\/jats:styled-content>\n also displayed a slowly inactivating outward current that was little affected by nifedipine, reduced by the\n \n Cl<\/jats:roman>\n <\/jats:styled-content>\n \u2013<\/jats:sup>\n channel blocker, niflumic acid (10\u2009\u03bc\n m<\/jats:sc>\n ) and blocked by\n \n \n Ba\n 2<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n +<\/jats:sup>\n or a conditioning depolarisation.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n PIC<\/jats:styled-content>\n s express both a small \u2018\n T<\/jats:styled-content>\n \u2010type\u2019\n \n \n Ca\n 2<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n +<\/jats:sup>\n channel current (\n \n I\n Ca<\/jats:sub>\n <\/jats:styled-content>\n ) and a large \u2018\n L<\/jats:styled-content>\n \u2010type\u2019\n \n I\n Ca<\/jats:sub>\n <\/jats:styled-content>\n .\n \n \n Ca\n 2<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n +<\/jats:sup>\n influx through \u2018\n T<\/jats:styled-content>\n \u2010type\u2019\n \n I\n Ca<\/jats:sub>\n <\/jats:styled-content>\n was an essential trigger for the activation of a\n \n \n Ca\n 2<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n +<\/jats:sup>\n \u2010activated\n C<\/jats:styled-content>\n l\n \u2013<\/jats:sup>\n \u2010selective current. The dependence of\n \n PIC\n \n Ca\n 2<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n +<\/jats:sup>\n signalling on \u2018\n T<\/jats:styled-content>\n \u2010type\u2019 and \u2018\n L<\/jats:styled-content>\n \u2010type\u2019\n \n I\n Ca<\/jats:sub>\n <\/jats:styled-content>\n is unique compared with other interstitial cells of the urogenital tract and may well be pharmaceutically exploitable.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/bju.12656","type":"journal-article","created":{"date-parts":[[2014,1,28]],"date-time":"2014-01-28T06:12:16Z","timestamp":1390889536000},"page":"436-446","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Voltage\u2010operated\n \n \n Ca\n 2<\/sup>\n <\/scp>\n <\/scp>\n +<\/sup>\n currents and\n \n \n Ca\n 2<\/sup>\n <\/scp>\n <\/scp>\n +<\/sup>\n \u2010activated\n \n Cl<\/scp>\n <\/scp>\n \u2013<\/sup>\n currents in single interstitial cells of the guinea\u2010pig 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