{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T03:35:33Z","timestamp":1762659333331,"version":"build-2065373602"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,8,13]],"date-time":"2014-08-13T00:00:00Z","timestamp":1407888000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004084","name":"Korea Science and Engineering Foundation","doi-asserted-by":"crossref","award":["2011-0015771"],"award-info":[{"award-number":["2011-0015771"]}],"id":[{"id":"10.13039\/501100004084","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Korea government (Ministry for Education, Science, and Technology)"}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2014,12]]},"abstract":"\n Objectives<\/jats:title>\n \n To examine the therapeutic effect of adenovirus encoding histone deacetylase 2 (\n HDAC2<\/jats:styled-content>\n ) small hairpin\n RNA<\/jats:styled-content>\n (\n Ad\u2010HDAC2 shRNA<\/jats:styled-content>\n ) in a rat model of\n P<\/jats:styled-content>\n eyronie's disease (\n PD<\/jats:styled-content>\n ) and to determine the mechanisms by which\n HDAC2<\/jats:styled-content>\n knockdown ameliorates fibrotic responses in primary fibroblasts derived from human\n PD<\/jats:styled-content>\n plaque.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n \n Rats were distributed into four groups (\n n<\/jats:italic>\n = 6 per group): age\u2010matched controls without treatment; rats in which\n PD<\/jats:styled-content>\n has been induced (\n PD<\/jats:styled-content>\n rats) without treatment;\n PD<\/jats:styled-content>\n rats receiving a single injection of control adenovirus encoding scrambled small hairpin RNA\n (Ad\u2010shRNA<\/jats:styled-content>\n ) (day 15; 1 \u00d7 10\n 8<\/jats:sup>\n pfu\/0.1\u2009mL phosphate\u2010buffered saline [\n PBS<\/jats:styled-content>\n ]); and\n PD<\/jats:styled-content>\n rats receiving a single injection of\n Ad\u2010HDAC2 shRNA<\/jats:styled-content>\n (day 15; 1 \u00d7 10\n 8<\/jats:sup>\n pfu\/0.1\u2009mL\n PBS<\/jats:styled-content>\n ) into the lesion.\n PD<\/jats:styled-content>\n \u2010like plaque was induced by repeated intratunical injections of 100 \u03bcL each of human fibrin and thrombin solutions on days 0 and 5. On day 30, the penis was harvested for histological examination. Fibroblasts isolated from human\n PD<\/jats:styled-content>\n plaque were pretreated with\n HDAC2<\/jats:styled-content>\n small interfering (si)\n RNA<\/jats:styled-content>\n (100 pmoL) and then stimulated with transforming growth factor (\n TGF<\/jats:styled-content>\n )\u2010\u03b21 (10\u2009ng\/mL) to determine hydroxyproline levels, procollagen\n mRNA<\/jats:styled-content>\n , apoptosis and protein expression of poly(\n ADP<\/jats:styled-content>\n \u2010ribose) polymerase 1 (\n PARP1<\/jats:styled-content>\n ) and cyclin\n D1<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n We observed that\n Ad\u2010HDAC2 shRNA<\/jats:styled-content>\n decreased inflammatory cell infiltration, reduced transnuclear expression of phospho\u2010\n S<\/jats:styled-content>\n mad3 and regressed fibrotic plaque of the tunica albuginea in\n PD<\/jats:styled-content>\n rats\n in vivo<\/jats:italic>\n .\n siRNA<\/jats:styled-content>\n \u2010mediated silencing of\n HDAC2<\/jats:styled-content>\n significantly decreased the\n TGF<\/jats:styled-content>\n \u2010\u03b21\u2010induced transdifferentiation of fibroblasts into myofibroblasts and collagen production, and induced apoptosis by downregulating the expression of\n PARP1<\/jats:styled-content>\n , and decreased the expression of cyclin\n D1<\/jats:styled-content>\n (a positive cell\u2010cycle regulator) in primary cultured fibroblasts derived from human\n PD<\/jats:styled-content>\n plaque\n in vitro<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Specific inhibition of\n HDAC2<\/jats:styled-content>\n with\n RNA<\/jats:styled-content>\n interference may represent a novel targeted therapy for\n PD<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/bju.12812","type":"journal-article","created":{"date-parts":[[2014,5,19]],"date-time":"2014-05-19T08:05:13Z","timestamp":1400486713000},"page":"926-936","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Silencing histone deacetylase 2 using small hairpin\n RNA<\/scp>\n induces regression of fibrotic plaque in a rat model of\n P<\/scp>\n eyronie's disease"],"prefix":"10.1111","volume":"114","author":[{"given":"Ki\u2010Dong","family":"Kwon","sequence":"first","affiliation":[{"name":"National Research Center for Sexual Medicine Department of Urology Inha University School of Medicine Incheon 400\u2010711 Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min Ji","family":"Choi","sequence":"additional","affiliation":[{"name":"National Research Center for Sexual Medicine Department of Urology Inha University School of Medicine Incheon 400\u2010711 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