{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T01:53:13Z","timestamp":1789609993179,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,1,26]],"date-time":"2015-01-26T00:00:00Z","timestamp":1422230400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia (FCT)","award":["SFRH\/BPD\/68716\/2010"],"award-info":[{"award-number":["SFRH\/BPD\/68716\/2010"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia (FCT)","award":["EXPL\/NEU-SCC\/0288\/2013"],"award-info":[{"award-number":["EXPL\/NEU-SCC\/0288\/2013"]}]}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2015,3]]},"abstract":"\n Objective<\/jats:title>\n \n To investigate transient receptor potential vanilloid 4 (\n TRPV4<\/jats:styled-content>\n ) expression in bladder afferents and study the effect of\n TRPV4<\/jats:styled-content>\n and\n TRPV1<\/jats:styled-content>\n antagonists, alone and in combination, in bladder hyperactivity and pain induced by cystitis.\n <\/jats:p>\n <\/jats:sec>\n \n Material and Methods<\/jats:title>\n \n TRPV4<\/jats:styled-content>\n expression in bladder afferents was analysed by immunohistochemistry in\n L6<\/jats:styled-content>\n dorsal root ganglia (\n DRG<\/jats:styled-content>\n ), labelled by fluorogold injected in the urinary bladder.\n TRPV4<\/jats:styled-content>\n and\n TRPV1<\/jats:styled-content>\n co\u2010expression was also investigated in L6\n DRG<\/jats:styled-content>\n neurones of control rats and in rats with lipopolysaccharide (\n LPS<\/jats:styled-content>\n )\u2010induced cystitis. The effect of\n TRPV4<\/jats:styled-content>\n antagonist\n RN1734<\/jats:styled-content>\n and\n TRPV1<\/jats:styled-content>\n antagonist\n SB366791<\/jats:styled-content>\n on bladder hyperactivity and pain induced by cystitis was assessed by cystometry and visceral pain behaviour tests, respectively.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n TRPV4<\/jats:styled-content>\n is expressed in sensory neurones that innervate the urinary bladder.\n TRPV4<\/jats:styled-content>\n \u2010positive bladder afferents represent a different population than the\n TRPV1<\/jats:styled-content>\n \u2010expressing bladder afferents, as their co\u2010localisation was minimal in control and inflamed rats. While low doses of\n RN1734<\/jats:styled-content>\n and\n SB366791<\/jats:styled-content>\n (176.7\u2009ng\/kg and 143.9\u2009ng\/kg, respectively) had no effect on bladder activity, the co\u2010administration of the two totally reversed bladder hyperactivity induced by\n LPS<\/jats:styled-content>\n . In these same doses, the antagonists partially reversed bladder pain behaviour induced by cystitis.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n TRPV4<\/jats:styled-content>\n and\n TRPV1<\/jats:styled-content>\n are present in different bladder afferent populations. The synergistic activity of antagonists for these receptors in very low doses may offer the opportunity to treat lower urinary tract symptoms while minimising the potential side\u2010effects of each drug.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/bju.12861","type":"journal-article","created":{"date-parts":[[2014,7,16]],"date-time":"2014-07-16T03:03:36Z","timestamp":1405479816000},"page":"452-460","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Co\u2010administration of transient receptor potential vanilloid 4 (\n TRPV4<\/scp>\n ) and\n TRPV1<\/scp>\n antagonists potentiate the effect of each drug in a rat model of cystitis"],"prefix":"10.1111","volume":"115","author":[{"given":"Ana","family":"Charrua","sequence":"first","affiliation":[{"name":"Department of Renal Urologic and Infectious Disease Porto Portugal"},{"name":"Department of Experimental Biology Faculty of Medicine of the University of Porto Porto Portugal"},{"name":"IBMC \u2013 Instituto de Biologia Molecular e Celular da Universidade do Porto Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9lia D.","family":"Cruz","sequence":"additional","affiliation":[{"name":"Department of Experimental Biology Faculty of Medicine of the University of Porto Porto Portugal"},{"name":"IBMC \u2013 Instituto de Biologia Molecular e Celular da Universidade do Porto Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dick","family":"Jansen","sequence":"additional","affiliation":[{"name":"Department of Urology Radboud University Nijmegen Medical Centre Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boy","family":"Rozenberg","sequence":"additional","affiliation":[{"name":"Department of Urology Radboud University Nijmegen Medical Centre Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Heesakkers","sequence":"additional","affiliation":[{"name":"Department of Urology Radboud University Nijmegen Medical Centre Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Cruz","sequence":"additional","affiliation":[{"name":"Department of Urology S. Jo\u00e3o Hospital Porto Portugal"},{"name":"Department of Renal Urologic and Infectious Disease Porto Portugal"},{"name":"IBMC \u2013 Instituto de Biologia Molecular e Celular da Universidade do Porto Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,1,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.12021"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2008.08.121"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2006.11.046"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1005333107"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(01)00496-1"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.231243698"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2004.08.007"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2009.08.022"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI31766"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.2008.951962"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1744-8069-5-5"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)65211-X"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.107.125435"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.21212"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2009.09.007"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1211946109"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0028-3908(03)00305-8"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)67495-2"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1053\/eujp.1998.0105"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(01)00275-5"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M302561200"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12031-012-9867-5"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-16499-6_17"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.108.139295"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2657-04.2004"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2009.03.085"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2012.09.055"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2008.11.004"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/HJH.0b013e328333af48"}],"container-title":["BJU International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbju.12861","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbju.12861","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bjui-journals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bju.12861","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T12:29:11Z","timestamp":1761740951000},"score":1,"resource":{"primary":{"URL":"https:\/\/bjui-journals.onlinelibrary.wiley.com\/doi\/10.1111\/bju.12861"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,1,26]]},"references-count":29,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,3]]}},"alternative-id":["10.1111\/bju.12861"],"URL":"https:\/\/doi.org\/10.1111\/bju.12861","archive":["Portico"],"relation":{},"ISSN":["1464-4096","1464-410X"],"issn-type":[{"value":"1464-4096","type":"print"},{"value":"1464-410X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,1,26]]},"assertion":[{"value":"2015-01-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}