{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T19:25:18Z","timestamp":1761765918719,"version":"build-2065373602"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,7,29]],"date-time":"2015-07-29T00:00:00Z","timestamp":1438128000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The Urological Foundation"}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2016,4]]},"abstract":"\n Objectives<\/jats:title>\n \n To evaluate the immunocytochemical detection of\n ERG<\/jats:styled-content>\n protein in exfoliated cells as a means of identifying patients with prostate cancer (\n PC<\/jats:styled-content>\n a) before prostate biopsy.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n \n Urine samples (30\u00a0mL) were collected after digital rectal examination (\n DRE<\/jats:styled-content>\n ) from 159 patients with an elevated age\u2010specific prostate\u2010specific antigen (\n PSA<\/jats:styled-content>\n ) and\/or an abnormal\n DRE<\/jats:styled-content>\n who underwent prostate biopsy. In all cases, exfoliated urinary cells from half of the urine sample underwent immunocytochemical assessment for\n ERG<\/jats:styled-content>\n protein expression. Exfoliated cells in the remaining half underwent assessment of\n \n TMPRSS<\/jats:styled-content>\n 2:\n ERG<\/jats:styled-content>\n <\/jats:italic>\n status using either nested reverse\u2010transcriptase (\n RT<\/jats:styled-content>\n )\u2010\n PCR<\/jats:styled-content>\n (151 cases) or fluorescence\n in\u00a0situ<\/jats:italic>\n hybridization (\n FISH<\/jats:styled-content>\n ; eight cases). Corresponding tissue samples were evaluated using\n FISH<\/jats:styled-content>\n to determine chromosomal gene fusion tissue status and immunohistochemistry (\n IHC<\/jats:styled-content>\n ) to determine\n ERG<\/jats:styled-content>\n protein expression. Results were correlated with clinicopathological variables.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The sensitivity and specificity of urinary\n ERG<\/jats:styled-content>\n immunocytochemistry (\n ICC<\/jats:styled-content>\n ) for\n PC<\/jats:styled-content>\n a were 22.7 and 100%, respectively.\n ERG ICC<\/jats:styled-content>\n results correlated with advanced tumour grade, stage and higher serum\n PSA<\/jats:styled-content>\n . In comparison, urine\n \n TMPRSS<\/jats:styled-content>\n 2:\n ERG<\/jats:styled-content>\n <\/jats:italic>\n transcript analysis had 27% sensitivity and 98% specificity for\n PC<\/jats:styled-content>\n a detection. On tissue\n IHC<\/jats:styled-content>\n ,\n ERG<\/jats:styled-content>\n staining was highly specific for\n PC<\/jats:styled-content>\n a. In all, 52% of cancers harboured foci of\n ERG<\/jats:styled-content>\n staining; however, only 46% of cancers that were found to have\n ERG<\/jats:styled-content>\n overexpression were positive on urine\n ICC<\/jats:styled-content>\n . The\n ERG ICC<\/jats:styled-content>\n results showed strong concordance with urinary\n RT<\/jats:styled-content>\n \u2010\n PCR<\/jats:styled-content>\n and\n FISH<\/jats:styled-content>\n , and tissue\n IHC<\/jats:styled-content>\n and\n FISH<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n This is the first study to show that cytological gene fusion detection using\n ICC<\/jats:styled-content>\n is feasible and identifies patients with adverse disease markers.\n ERG ICC<\/jats:styled-content>\n was highly specific, but this technique was less sensitive than\n RT<\/jats:styled-content>\n \u2010\n PCR<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/bju.13184","type":"journal-article","created":{"date-parts":[[2015,6,1]],"date-time":"2015-06-01T02:12:30Z","timestamp":1433124750000},"page":"686-696","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Immunocytochemical detection of\n ERG<\/scp>\n expression in exfoliated urinary cells identifies with high specificity patients with prostate cancer"],"prefix":"10.1111","volume":"117","author":[{"given":"Raj P.","family":"Pal","sequence":"first","affiliation":[{"name":"Department of Cancer Studies and Molecular Medicine University of Leicester Leicester UK"},{"name":"Department of Urology University Hospitals of Leicester NHS Trust Leicester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger C.","family":"Kockelbergh","sequence":"additional","affiliation":[{"name":"Department of Urology University Hospitals of Leicester NHS Trust Leicester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John Howard","family":"Pringle","sequence":"additional","affiliation":[{"name":"Department of Cancer Studies and Molecular Medicine University of Leicester Leicester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lara","family":"Cresswell","sequence":"additional","affiliation":[{"name":"Department of Cytogenetics University Hospitals of Leicester NHS Trust Leicester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger","family":"Hew","sequence":"additional","affiliation":[{"name":"Department of Cellular Pathology University Hospitals of Leicester NHS Trust Leicester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John P.","family":"Dormer","sequence":"additional","affiliation":[{"name":"Department of Cellular Pathology University Hospitals of Leicester NHS Trust Leicester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colin","family":"Cooper","sequence":"additional","affiliation":[{"name":"Department of Cancer Genetics University of East Anglia Norwich UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John Kilian","family":"Mellon","sequence":"additional","affiliation":[{"name":"Department of Urology University Hospitals of Leicester NHS Trust Leicester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julian G.","family":"Barwell","sequence":"additional","affiliation":[{"name":"Department of Genetics University of Leicester Leicester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward J.","family":"Hollox","sequence":"additional","affiliation":[{"name":"Department of Genetics University of Leicester Leicester 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