{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T04:55:36Z","timestamp":1781844936376,"version":"3.54.5"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,8,19]],"date-time":"2015-08-19T00:00:00Z","timestamp":1439942400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ministry of Education, Culture, Sport, Science and Technology of the Japanese Government","award":["40159588"],"award-info":[{"award-number":["40159588"]}]},{"name":"Ministry of Education, Culture, Sport, Science and Technology of the Japanese Government","award":["80595257"],"award-info":[{"award-number":["80595257"]}]},{"name":"Gester Foundation"}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2016,5]]},"abstract":"\n Objective<\/jats:title>\n \n To determine if inhibition of the endocannabinoid\u2010degrading enzyme fatty acid amide hydrolase (\n FAAH<\/jats:styled-content>\n ) can counteract the changes in urodynamic variables and bladder afferent activities induced by intravesical prostaglandin E\n 2<\/jats:sub>\n (\n PGE<\/jats:styled-content>\n 2<\/jats:sub>\n ) instillation in rats.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and methods<\/jats:title>\n \n In female Sprague\u2013Dawley rats we studied the effects of\n URB<\/jats:styled-content>\n 937, a peripherally restricted\n FAAH<\/jats:styled-content>\n inhibitor, on single\u2010unit afferent activity (\n SAA<\/jats:styled-content>\n ) during\n PGE<\/jats:styled-content>\n 2<\/jats:sub>\n \u2010induced bladder overactivity (\n BO<\/jats:styled-content>\n ).\n SAA<\/jats:styled-content>\n measurements were made in urethane\u2010anaesthetised rats and A\u03b4\u2010 and C\u2010fibres were identified by electrical stimulation of the pelvic nerve and by bladder distention. Cystometry (\n CMG<\/jats:styled-content>\n ) in conscious animals and during\n SAA<\/jats:styled-content>\n measurements was performed during intravesical instillation of\n PGE<\/jats:styled-content>\n 2<\/jats:sub>\n (50 or 100\u00a0\u03bc\n m<\/jats:sc>\n ) after intravenous administration of\n URB<\/jats:styled-content>\n 937 (0.1 and 1\u00a0mg\/kg) or vehicle. In separate experiments, the comparative expressions of\n FAAH<\/jats:styled-content>\n and cannabinoid receptors,\n CB<\/jats:styled-content>\n 1<\/jats:sub>\n and\n CB<\/jats:styled-content>\n 2<\/jats:sub>\n , in microsurgically removed L6 dorsal root ganglion (\n DRG<\/jats:styled-content>\n ) were studied by immunofluorescence.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n During\n CMG<\/jats:styled-content>\n , 1\u00a0mg\/kg\n URB<\/jats:styled-content>\n 937, but not vehicle or 0.1\u00a0mg\/kg\n URB<\/jats:styled-content>\n 937, counteracted the\n PGE<\/jats:styled-content>\n 2<\/jats:sub>\n \u2010induced changes in urodynamic variables.\n PGE<\/jats:styled-content>\n 2<\/jats:sub>\n increased the\n SAA<\/jats:styled-content>\n s of C\u2010fibres, but not A\u03b4\u2010fibres.\n URB<\/jats:styled-content>\n 937 (1\u00a0mg\/kg) depressed A\u03b4\u2010fibre\n SAA<\/jats:styled-content>\n and abolished the facilitated C\u2010fibre\n SAA<\/jats:styled-content>\n induced by\n PGE<\/jats:styled-content>\n 2<\/jats:sub>\n . The\n DRG<\/jats:styled-content>\n nerve cells showed strong staining for\n FAAH<\/jats:styled-content>\n ,\n CB<\/jats:styled-content>\n 1<\/jats:sub>\n and\n CB<\/jats:styled-content>\n 2<\/jats:sub>\n , with a mean (\n sem<\/jats:sc>\n ) of 77 (2)% and 87 (3)% of\n FAAH<\/jats:styled-content>\n \u2010positive nerve cell bodies co\u2010expressing\n CB<\/jats:styled-content>\n 1<\/jats:sub>\n or\n CB<\/jats:styled-content>\n 2<\/jats:sub>\n immunofluorescence, respectively.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n The present results show that\n URB<\/jats:styled-content>\n 937, a peripherally restricted\n FAAH<\/jats:styled-content>\n inhibitor, reduces\n BO<\/jats:styled-content>\n and C\u2010fibre hyperactivity in the rat bladder provoked by\n PGE<\/jats:styled-content>\n 2<\/jats:sub>\n , suggesting an important role of the peripheral endocannabinoid system in\n BO<\/jats:styled-content>\n and hypersensitivity.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/bju.13223","type":"journal-article","created":{"date-parts":[[2015,7,18]],"date-time":"2015-07-18T05:03:23Z","timestamp":1437195803000},"page":"821-828","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["URB<\/scp>\n 937, a peripherally restricted inhibitor for fatty acid amide hydrolase, reduces prostaglandin E\n 2<\/sub>\n \u2010induced bladder overactivity and hyperactivity of bladder mechano\u2010afferent nerve fibres in rats"],"prefix":"10.1111","volume":"117","author":[{"given":"Naoki","family":"Aizawa","sequence":"first","affiliation":[{"name":"Department of Continence Medicine The University of Tokyo Graduate School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgio","family":"Gandaglia","sequence":"additional","affiliation":[{"name":"Division of Oncology\/Unit of Urology Urological Research Institute IRCCS Ospedale San Raffaele Milan 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