{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:13:57Z","timestamp":1791346437252,"version":"4.3.1"},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,10,5]],"date-time":"2016-10-05T00:00:00Z","timestamp":1475625600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R56DK105097"],"award-info":[{"award-number":["R56DK105097"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2017,2]]},"abstract":"\n Objective<\/jats:title>\n \n To study and compare the function and structure of the urethral sphincter in female Zucker lean (\n ZL<\/jats:styled-content>\n ) and Zucker fatty (\n ZF<\/jats:styled-content>\n ) rats and to assess the viability of\n ZF<\/jats:styled-content>\n fats as a model for female obesity\u2010associated stress urinary incontinence (\n SUI<\/jats:styled-content>\n ).\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n \n Two study arms were created: a\n ZL<\/jats:styled-content>\n arm including 16\u2010week\u2010old female\n ZL<\/jats:styled-content>\n rats (\n ZUC<\/jats:styled-content>\n \u2010Lepr\n fa<\/jats:sup>\n 186;\n n<\/jats:italic>\n = 12) and a\n ZF<\/jats:styled-content>\n arm including 16\u2010week\u2010old female\n ZF<\/jats:styled-content>\n rats (\n ZUC<\/jats:styled-content>\n \u2010Lepr\n fa<\/jats:sup>\n 185;\n n<\/jats:italic>\n = 12). I.p. insulin tolerance testing was carried out before functional study. Metabolic cages, conscious cystometry and leak point pressure (\n LPP<\/jats:styled-content>\n ) assessments were conducted. Urethral tissues were harvested for immunofluorescence staining to check intramyocellular lipid (\n IMCL<\/jats:styled-content>\n ) and sphincter muscle (smooth muscle and striated muscle) composition.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The\n ZF<\/jats:styled-content>\n rats had insulin resistance, a greater voiding frequency and lower\n LPP<\/jats:styled-content>\n compared with\n ZL<\/jats:styled-content>\n rats (\n P<\/jats:italic>\n < 0.05), with more\n IMCL<\/jats:styled-content>\n deposition localized in the urethral striated muscle fibres of the\n ZF<\/jats:styled-content>\n rats (\n P<\/jats:italic>\n < 0.05). The thickness of the striated muscle layer and the ratio of striated muscle to smooth muscle were lower in\n ZF<\/jats:styled-content>\n than in\n ZL<\/jats:styled-content>\n rats.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Obesity impairs urethral sphincter function via\n IMCL<\/jats:styled-content>\n deposition and leads to atrophy and distortion of urethral striated muscle. The\n ZF<\/jats:styled-content>\n rats could be a consistent and reliable animal model in which to study obesity\u2010associated\n SUI<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/bju.13661","type":"journal-article","created":{"date-parts":[[2016,9,21]],"date-time":"2016-09-21T05:26:42Z","timestamp":1474435602000},"page":"317-324","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Transgenic animal model for studying the mechanism of obesity\u2010associated stress urinary incontinence"],"prefix":"10.1111","volume":"119","author":[{"given":"Lin","family":"Wang","sequence":"first","affiliation":[{"name":"Knuppe Molecular Urology Laboratory Department of Urology School of Medicine University of California San Francisco CA USA"},{"name":"Department of Urology Peking University First Hospital Peking University Beijing 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