{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T16:05:57Z","timestamp":1792944357729,"version":"4.5.0"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,6,29]],"date-time":"2017-06-29T00:00:00Z","timestamp":1498694400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U01\u2010DK\u2010082315"],"award-info":[{"award-number":["U01\u2010DK\u2010082315"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K08\u2010DK\u2010094964"],"award-info":[{"award-number":["K08\u2010DK\u2010094964"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81400758"],"award-info":[{"award-number":["81400758"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","award":["2013081110286"],"award-info":[{"award-number":["2013081110286"]}],"id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      To investigate whether treatment with anti\u2010vascular endothelial growth factor (\n                      <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content>\n                      )\u2010neutralizing antibodies can reduce pain and voiding dysfunction in the cyclophosphamide (\n                      <jats:styled-content style=\"fixed-case\">CYP<\/jats:styled-content>\n                      ) cystitis model of bladder pain in mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Adult female mice received anti\u2010\n                      <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content>\n                      \u2010neutralizing antibodies (10\u00a0mg\/kg i.p. B20\u20104.1.1\n                      <jats:styled-content style=\"fixed-case\">VEGF mA<\/jats:styled-content>\n                      b) or saline (control) pre\u2010treatment, followed by\n                      <jats:styled-content style=\"fixed-case\">CYP<\/jats:styled-content>\n                      (150\u00a0mg\/kg i.p.) to induce acute cystitis. Pelvic nociceptive responses were assessed by applying von Frey filaments to the pelvic area. Spontaneous micturition was assessed using the void spot assay.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Systemic anti\u2010\n                      <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content>\n                      \u2010neutralizing antibody treatment significantly reduced the pelvic nociceptive response to\n                      <jats:styled-content style=\"fixed-case\">CYP<\/jats:styled-content>\n                      cystitis compared with control (saline). In the anti\u2010\n                      <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content>\n                      pre\u2010treatment group, there was a significant increase in pelvic hypersensitivity, measured by the area under the curve (\n                      <jats:styled-content style=\"fixed-case\">AUC<\/jats:styled-content>\n                      ) using von Frey filaments at 5\u00a0h post\u2010\n                      <jats:styled-content style=\"fixed-case\">CYP<\/jats:styled-content>\n                      administration (\n                      <jats:italic>P<\/jats:italic>\n                      = 0.004); however, by 48\u00a0h and 96\u00a0h post\u2010\n                      <jats:styled-content style=\"fixed-case\">CYP<\/jats:styled-content>\n                      administration, pelvic hypersensitivity had reduced by 54% and 47%, respectively, compared with the 5\u00a0h post\u2010\n                      <jats:styled-content style=\"fixed-case\">CYP<\/jats:styled-content>\n                      administration time point, and were no longer significantly different from baseline (\n                      <jats:italic>P<\/jats:italic>\n                      = 0.22 and 0.17, respectively). There was no difference in urinary frequency and mean voided volume between the two pre\u2010treatment groups.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Systemic blockade of\n                      <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content>\n                      signalling with anti\u2010\n                      <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content>\n                      \u2010neutralizing antibodies was effective in reducing pelvic\/bladder pain in the\n                      <jats:styled-content style=\"fixed-case\">CYP<\/jats:styled-content>\n                      cystitis model of bladder pain. Our data support the further investigation of the use of anti\u2010\n                      <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content>\n                      antibodies to manage bladder pain or visceral pain.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bju.13924","type":"journal-article","created":{"date-parts":[[2017,6,5]],"date-time":"2017-06-05T11:36:42Z","timestamp":1496662602000},"page":"576-583","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Anti\u2010vascular endothelial growth factor treatment decreases bladder pain in cyclophosphamide cystitis: a Multidisciplinary Approach to the Study of Chronic Pelvic Pain (\n                    <scp>MAPP<\/scp>\n                    ) Research Network animal model study"],"prefix":"10.1111","volume":"120","author":[{"given":"H. Henry","family":"Lai","sequence":"first","affiliation":[{"name":"Division of Urologic Surgery Department of Surgery Washington University School of Medicine St Louis MO USA"},{"name":"Washington University Pain Center Department of Anesthesiology Washington University School of Medicine St Louis MO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baixin","family":"Shen","sequence":"additional","affiliation":[{"name":"Division of Urologic Surgery Department of Surgery Washington University School of Medicine St Louis MO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pooja","family":"Vijairania","sequence":"additional","affiliation":[{"name":"Division of Urologic Surgery Department of Surgery Washington University School of Medicine St Louis MO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Division of Urologic Surgery Department of Surgery Washington University School of Medicine St Louis MO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherri K.","family":"Vogt","sequence":"additional","affiliation":[{"name":"Washington University Pain Center Department of Anesthesiology Washington University School of Medicine St Louis MO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"IV","given":"Robert W.","family":"Gereau","sequence":"additional","affiliation":[{"name":"Washington University Pain Center Department of Anesthesiology Washington University School of Medicine St Louis MO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,6,29]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2015.01.086"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2015.03.007"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0603-669"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000135009.55905.cb"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2011.05.050"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/bju.13832"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2009.08467.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2013.09.003"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6793-12-15"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.90305.2008"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M507783200"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000000224"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2011.05.017"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00074.2014"},{"key":"e_1_2_6_16_1","unstructured":"Bivacizumab treatment for metastatic colorectal cancer advanced nonsquamous non\u2010small cell lung cancer metastatic kidney cancer and gioblastoma.http:\/\/www.avastin.com\/patient. 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