{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T05:35:08Z","timestamp":1791956108143,"version":"4.3.4"},"reference-count":0,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,8,22]],"date-time":"2017-08-22T00:00:00Z","timestamp":1503360000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["R01DK067223"],"award-info":[{"award-number":["R01DK067223"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["F32DK100082"],"award-info":[{"award-number":["F32DK100082"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2017,12]]},"abstract":"\n Objectives<\/jats:title>\n \n To evaluate neuronal nitric oxide (\n NO<\/jats:styled-content>\n ) synthase (\n nNOS<\/jats:styled-content>\n ) phosphorylation,\n nNOS<\/jats:styled-content>\n uncoupling, and oxidative stress in the penis and major pelvic ganglia (\n MPG<\/jats:styled-content>\n ), before and after the administration of the\n cAMP<\/jats:styled-content>\n \u2010dependent protein kinase A (\n PKA<\/jats:styled-content>\n ) agonist colforsin in a rat model of bilateral cavernous nerve injury (\n BCNI<\/jats:styled-content>\n ),which mimics nerve injury after prostatectomy.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n \n Adult male Sprague\u2013Dawley rats were divided into\n BCNI<\/jats:styled-content>\n and sham\u2010operated groups. Each group included two subgroups: vehicle and colforsin (0.1 mg\/kg\/day i.p.). After 3 days, erectile function (intracavernosal pressure) was measured and penis and\n MPG<\/jats:styled-content>\n were collected for molecular analyses of phospho (P)\u2010\n nNOS<\/jats:styled-content>\n (Ser\u20101412 and Ser\u2010847), total\n nNOS<\/jats:styled-content>\n ,\n nNOS<\/jats:styled-content>\n uncoupling, binding of protein inhibitor of\n nNOS<\/jats:styled-content>\n (\n PIN<\/jats:styled-content>\n ) to\n nNOS<\/jats:styled-content>\n , gp91\n phox<\/jats:sup>\n subunit of\n NADPH<\/jats:styled-content>\n oxidase, active caspase 3,\n PKA<\/jats:styled-content>\n catalytic subunit \u03b1 (\n PKA<\/jats:styled-content>\n \u2010C\u03b1; by Western blot) and oxidative stress (hydrogen peroxide [H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n ] and superoxide by Western blot and microdialysis method).\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Erectile function was decreased 3 days after\n BCNI<\/jats:styled-content>\n and normalized by colforsin.\n nNOS<\/jats:styled-content>\n phosphorylation on both positive (Ser\u20101412) and negative (Ser\u2010847) regulatory sites, and\n nNOS<\/jats:styled-content>\n uncoupling, were increased after\n BCNI<\/jats:styled-content>\n in the penis and\n MPG<\/jats:styled-content>\n , and normalized by colforsin. H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n and total reactive oxygen species production were increased in the penis after\n BCNI<\/jats:styled-content>\n and normalized by colforsin. Protein expression of gp91\n phox<\/jats:sup>\n was increased in the\n MPG<\/jats:styled-content>\n after\n BCNI<\/jats:styled-content>\n and was normalized by colforsin treatment. Binding of\n PIN<\/jats:styled-content>\n to\n nNOS<\/jats:styled-content>\n was increased in the penis after\n BCNI<\/jats:styled-content>\n and was normalized by colforsin treatment. Protein expression of active Caspase 3 was increased in the\n MPG<\/jats:styled-content>\n after\n BCNI<\/jats:styled-content>\n and was normalized by colforsin treatment. Protein expression of\n PKA<\/jats:styled-content>\n \u2010C\u03b1 was decreased in the penis after\n BCNI<\/jats:styled-content>\n and normalized by colforsin.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Collectively,\n BCNI<\/jats:styled-content>\n impairs\n nNOS<\/jats:styled-content>\n function in the penis and\n MPG<\/jats:styled-content>\n by mechanisms involving its phosphorylation and uncoupling in association with increased oxidative stress, resulting in erectile dysfunction.\n PKA<\/jats:styled-content>\n activation by colforsin reverses these molecular changes and preserves penile erection in the face of\n BCNI<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/bju.13981","type":"journal-article","created":{"date-parts":[[2017,8,7]],"date-time":"2017-08-07T03:56:39Z","timestamp":1502078199000},"page":"861-872","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["cAMP<\/scp>\n \u2010dependent post\u2010translational modification of neuronal nitric oxide synthase neuroprotects penile erection in rats"],"prefix":"10.1111","volume":"120","author":[{"given":"Serkan","family":"Karakus","sequence":"first","affiliation":[{"name":"The James Buchanan Brady Urological Institute and Department of Urology Johns Hopkins University School of Medicine Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biljana","family":"Musicki","sequence":"additional","affiliation":[{"name":"The James Buchanan Brady Urological Institute and Department of Urology Johns Hopkins University School of Medicine Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin D.","family":"La Favor","sequence":"additional","affiliation":[{"name":"The James Buchanan Brady Urological Institute and Department of Urology Johns Hopkins University School of Medicine Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur L.","family":"Burnett","sequence":"additional","affiliation":[{"name":"The James Buchanan Brady Urological Institute and Department of Urology Johns Hopkins University School of Medicine Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,8,22]]},"container-title":["BJU International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbju.13981","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bjui-journals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bju.13981","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:15:23Z","timestamp":1761743723000},"score":1,"resource":{"primary":{"URL":"https:\/\/bjui-journals.onlinelibrary.wiley.com\/doi\/10.1111\/bju.13981"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,22]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1111\/bju.13981"],"URL":"https:\/\/doi.org\/10.1111\/bju.13981","archive":["Portico"],"relation":{},"ISSN":["1464-4096","1464-410X"],"issn-type":[{"value":"1464-4096","type":"print"},{"value":"1464-410X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,22]]},"assertion":[{"value":"2017-08-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}