{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T01:54:03Z","timestamp":1789610043122,"version":"build-2803163510"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,10,26]],"date-time":"2018-10-26T00:00:00Z","timestamp":1540512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DK082644"],"award-info":[{"award-number":["DK082644"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007144","name":"University of Houston","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007144","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000883","name":"Brown Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000883","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2019,3]]},"abstract":"\n Objectives<\/jats:title>\n \n To evaluate the role that intravesical P2X2\/3 purinergic receptors (P2X2\/3Rs) play in early and advanced neurogenic lower urinary tract (\n LUT<\/jats:styled-content>\n ) dysfunction after contusion spinal cord injury (\n SCI<\/jats:styled-content>\n ) in female rats.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n \n Female Sprague\u2010Dawley rats received a thoracic Th8\/Th9 spinal cord contusion with either force of 100 kDy (cN); moderate) or 150 kDy (cN; severe); Sham rats had no injury. Evaluations on urethane\u2010anesthetised rats were conducted at either 2 or 4 weeks after\n SCI<\/jats:styled-content>\n .\n LUT<\/jats:styled-content>\n electrical signals and changes in bladder pressure were simultaneously recorded using cystometry and a set of custom\u2010made flexible microelectrodes, before and after intravesical application of the P2X2\/3R antagonist\n AF<\/jats:styled-content>\n \u2010353 (10 \u03bcM), to determine the contribution of P2X2\/3R\u2010mediated\n LUT<\/jats:styled-content>\n modulation.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Severe\n SCI<\/jats:styled-content>\n significantly increased bladder contraction frequency, and reduced both bladder pressure amplitude and intraluminal\u2010pressure high\u2010frequency oscillations (\n IPHFO<\/jats:styled-content>\n ). Intravesical P2X2\/3R inhibition did not modify bladder pressure or\n IPHFO<\/jats:styled-content>\n in the Sham and moderate\n \u2010SCI<\/jats:styled-content>\n rats, although did increase the intercontractile interval (ICI). At 2 weeks after\n SCI<\/jats:styled-content>\n , the Sham and moderate\u2010\n SCI<\/jats:styled-content>\n rats had significant\n LUT<\/jats:styled-content>\n electromyographic activity during voiding, with a noticeable reduction in\n LUT<\/jats:styled-content>\n electrical signals seen at 4 weeks after\n SCI<\/jats:styled-content>\n . Intravesical inhibition of P2X2\/3R increased the ICI in the Sham and moderate\n \u2010SCI<\/jats:styled-content>\n rats at both time\u2010points, but had no effect on rats with severe\n SCI<\/jats:styled-content>\n . The external urethral sphincter (\n EUS<\/jats:styled-content>\n ) showed strong and P2X2\/3R\u2010independent electrical signals in the Sham and moderate\u2010\n SCI<\/jats:styled-content>\n rats in the early\n SCI<\/jats:styled-content>\n stage. At 4 weeks after\n SCI<\/jats:styled-content>\n , the responsiveness of the\n EUS<\/jats:styled-content>\n was significantly attenuated, independently of\n SCI<\/jats:styled-content>\n intensity.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n This study shows that electrophysiological properties of the\n LUT<\/jats:styled-content>\n are progressively impaired depending on\n SCI<\/jats:styled-content>\n intensity and that intravesical P2X2\/3R inhibition can attenuate electrical activity in the neurogenic\n LUT<\/jats:styled-content>\n at early, but not at semi\u2010chronic\n SCI<\/jats:styled-content>\n . This translational study should be useful for planning clinical evaluations.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/bju.14561","type":"journal-article","created":{"date-parts":[[2018,9,26]],"date-time":"2018-09-26T03:36:19Z","timestamp":1537932979000},"page":"538-547","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Modulatory effects of intravesical P2X2\/3 purinergic receptor inhibition on lower urinary tract electromyographic properties and voiding function of female rats with moderate or severe spinal cord injury"],"prefix":"10.1111","volume":"123","author":[{"given":"Betsy H.","family":"Salazar","sequence":"first","affiliation":[{"name":"Regenerative Medicine and Tissue Engineering Program\u2010Urology Houston Methodist Research Institute Houston TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristopher 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