{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T14:37:54Z","timestamp":1761748674658,"version":"build-2065373602"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,2,17]],"date-time":"2019-02-17T00:00:00Z","timestamp":1550361600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000009","name":"Foundation for the National Institutes of Health","doi-asserted-by":"publisher","award":["R01 DK098361"],"award-info":[{"award-number":["R01 DK098361"]}],"id":[{"id":"10.13039\/100000009","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003122","name":"Ferring","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003122","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      To investigate the influence of low\u2010dose sildenafil, a phosphodiesterase type 5 inhibitor (\n                      <jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content>\n                      5\u2010I), on the function of the mouse lower urinary tract (\n                      <jats:styled-content style=\"fixed-case\">LUT<\/jats:styled-content>\n                      ).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Adult male mice were decerebrated and arterially perfused with a carbogenated Ringer's solution to establish the decerebrate arterially perfused mouse (\n                      <jats:styled-content style=\"fixed-case\">DAPM<\/jats:styled-content>\n                      ). To allow distinction between central neural and peripheral actions of sildenafil, experiments were conducted in both the\n                      <jats:styled-content style=\"fixed-case\">DAPM<\/jats:styled-content>\n                      and in a \u2018pithed\u2019\n                      <jats:styled-content style=\"fixed-case\">DAPM,<\/jats:styled-content>\n                      which has no functional brainstem or spinal cord. The action of systemic and intrathecal sildenafil on micturition was assessed in urethane\u2010anaesthetised mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In the\n                      <jats:styled-content style=\"fixed-case\">DAPM<\/jats:styled-content>\n                      , systemic perfusion of sildenafil (30 p\n                      <jats:sc>m<\/jats:sc>\n                      ) decreased the voiding threshold pressure [to a mean (\n                      <jats:sc>sem<\/jats:sc>\n                      ) 84.7 (3.8)% of control] and increased bladder compliance [to a mean (\n                      <jats:sc>sem<\/jats:sc>\n                      ) 140.2 (8.3)% of control, an effect replicated in the pithed\n                      <jats:styled-content style=\"fixed-case\">DAPM]<\/jats:styled-content>\n                      . Sildenafil was without effect on most voiding variables but significantly increased the number of bursts of the external urethral sphincter (EUS) per void in\n                      <jats:styled-content style=\"fixed-case\">DAPM<\/jats:styled-content>\n                      [to a mean (\n                      <jats:sc>sem<\/jats:sc>\n                      ) 130.1 (6.9)% of control at 30 p\n                      <jats:sc>m]<\/jats:sc>\n                      and in urethane\u2010anaesthetised mice [to a mean (\n                      <jats:sc>sem<\/jats:sc>\n                      ) 117.5 (5.8)% of control at 14 ng\/kg]. Sildenafil (10 and 30 p\n                      <jats:sc>m<\/jats:sc>\n                      ) increased pelvic afferent activity during both bladder filling and the isovolumetric phase [to a mean (\n                      <jats:sc>sem<\/jats:sc>\n                      ) 205.4 (30.2)% of control at 30 p\n                      <jats:sc>m]<\/jats:sc>\n                      . Intrathecal application of sildenafil (5 \u03bcL of either 150 p\n                      <jats:sc>m<\/jats:sc>\n                      or 1.5 n\n                      <jats:sc>m<\/jats:sc>\n                      ) did not alter cystometry and\n                      <jats:styled-content style=\"fixed-case\">EUS<\/jats:styled-content>\n                      \u2010\n                      <jats:styled-content style=\"fixed-case\">electromyography<\/jats:styled-content>\n                      variables in urethane\u2010anaesthetised mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Low\u2010dose sildenafil increases bladder compliance, increases pelvic nerve afferent activity, and augments the bursting activity of the EUS. We propose that the novel actions on afferent traffic and sphincter control may contribute to its beneficial actions to restore storage and voiding efficiency in\n                      <jats:styled-content style=\"fixed-case\">LUT<\/jats:styled-content>\n                      dysfunction.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bju.14664","type":"journal-article","created":{"date-parts":[[2019,1,12]],"date-time":"2019-01-12T08:50:29Z","timestamp":1547283029000},"page":"163-173","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Sildenafil, a phosphodiesterase type 5 inhibitor, augments sphincter bursting and bladder afferent activity to enhance storage function and voiding efficiency in mice"],"prefix":"10.1111","volume":"124","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4433-370X","authenticated-orcid":false,"given":"Hiroki","family":"Ito","sequence":"first","affiliation":[{"name":"School of Physiology, Pharmacology and Neuroscience Faculty of Biomedical Sciences University of Bristol Bristol UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7320-4931","authenticated-orcid":false,"given":"Basu","family":"Chakrabarty","sequence":"additional","affiliation":[{"name":"School of Physiology, Pharmacology and Neuroscience Faculty of Biomedical Sciences University of Bristol Bristol UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6230-2552","authenticated-orcid":false,"given":"Marcus J.","family":"Drake","sequence":"additional","affiliation":[{"name":"School of Physiology, Pharmacology and Neuroscience Faculty of Biomedical Sciences University of Bristol Bristol UK"},{"name":"Bristol Medical School and Bristol Urological Institute Bristol UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher H.","family":"Fry","sequence":"additional","affiliation":[{"name":"School of Physiology, Pharmacology and Neuroscience Faculty of Biomedical Sciences University of Bristol Bristol UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4780-7592","authenticated-orcid":false,"given":"Anthony J.","family":"Kanai","sequence":"additional","affiliation":[{"name":"Department of Medicine and Pharmacology and Chemical Biology University of Pittsburgh Pittsburgh PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony E.","family":"Pickering","sequence":"additional","affiliation":[{"name":"School of Physiology, Pharmacology and Neuroscience Faculty of Biomedical Sciences University of Bristol Bristol UK"},{"name":"Bristol Anaesthetic, Pain and Critical Care Sciences Translational Health Sciences Bristol Medical School University of Bristol Bristol UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,2,17]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.110.002907"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2015.12.048"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2010.07.508"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2010.09698.x"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2012.02.033"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jsm.12499"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/iju.12699"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0107593"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.00748.x"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2010.10.035"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2012-1431"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.22686"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05453"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.23471"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2017.00049"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2010.03.110"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/004982599238687"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(01)01471-6"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2012.07.025"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2011.00062"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.113.207340"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(00)00457-X"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2012.11255.x"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/luts.12095"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/bju.13225"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2013.03.112"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00111.2010"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1838(89)90130-6"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1982.sp014271"}],"container-title":["BJU International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbju.14664","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bjui-journals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bju.14664","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:41:52Z","timestamp":1761745312000},"score":1,"resource":{"primary":{"URL":"https:\/\/bjui-journals.onlinelibrary.wiley.com\/doi\/10.1111\/bju.14664"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,17]]},"references-count":29,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,7]]}},"alternative-id":["10.1111\/bju.14664"],"URL":"https:\/\/doi.org\/10.1111\/bju.14664","archive":["Portico"],"relation":{},"ISSN":["1464-4096","1464-410X"],"issn-type":[{"type":"print","value":"1464-4096"},{"type":"electronic","value":"1464-410X"}],"subject":[],"published":{"date-parts":[[2019,2,17]]},"assertion":[{"value":"2019-02-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}