{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T13:42:14Z","timestamp":1791639734454,"version":"4.3.4"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,4,17]],"date-time":"2019-04-17T00:00:00Z","timestamp":1555459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      To report the first application of\n                      <jats:italic>ex vivo<\/jats:italic>\n                      fluorescence confocal microscopy (FCM) \u2010 a novel optical technology that is capable of providing fast microscopic imaging of unfixed tissue specimens\u2010 in the urological field assessing its diagnostic accuracy for non neoplastic and cancerous prostate tissue (prostatic adenocarcinoma) compared to the 'gold standard' histopathological diagnoses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Patients and methods<\/jats:title>\n                    <jats:p>In all, 89 specimens from 13 patients with clinically localised prostate cancer were enrolled into the study. All patients underwent robot\u2010assisted laparoscopic radical prostatectomy with fresh prostatic tissue biopsies taken at the end of each intervention using an 18\u2010G biopsy punch. Specimens were randomly assigned to the three collaborating pathologists for evaluation. Intra\u2010 and inter\u2010observer agreement was tested by the means of Cohen's \u03ba. The diagnostic performance was evaluated on receiver operating characteristic curve analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The overall diagnostic agreement between\n                      <jats:styled-content style=\"fixed-case\">FCM<\/jats:styled-content>\n                      and histopathological diagnoses was substantial with a 91% correct diagnosis (\u03ba\u00a0=\u00a00.75) and an area under the curve of 0.884 (95%\n                      <jats:styled-content style=\"fixed-case\">confidence interval<\/jats:styled-content>\n                      0.840\u20130.920), 83.33% sensitivity, and 93.53% specificity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      <jats:styled-content style=\"fixed-case\">FCM<\/jats:styled-content>\n                      seems to be a promising tool for enhanced specimens' reporting performance, given its simple application and very rapid microscopic image generation (&lt;5\u00a0min\/specimen). This technique may potentially be used for intraoperative pathological specimens' analysis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bju.14754","type":"journal-article","created":{"date-parts":[[2019,3,25]],"date-time":"2019-03-25T15:25:33Z","timestamp":1553527533000},"page":"469-476","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":85,"title":["<i>Ex vivo<\/i>\n                    fluorescence confocal microscopy: the first application for real\u2010time pathological examination of prostatic tissue"],"prefix":"10.1111","volume":"124","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1597-9595","authenticated-orcid":false,"given":"Stefano","family":"Puliatti","sequence":"first","affiliation":[{"name":"Department of Urology University of Modena and Reggio Emilia  Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Bertoni","sequence":"additional","affiliation":[{"name":"Department of Surgical, Medical, Dental and Morphological Sciences with Interest Transplant, Oncological and Regenerative Medicine University of Modena and Reggio Emilia Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4209-8670","authenticated-orcid":false,"given":"Giacomo M.","family":"Pirola","sequence":"additional","affiliation":[{"name":"Department of Urology University of Modena and Reggio Emilia  Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Azzoni","sequence":"additional","affiliation":[{"name":"Department of Surgical, Medical, Dental and Morphological Sciences with Interest Transplant, Oncological and Regenerative Medicine University of Modena and Reggio Emilia Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigi","family":"Bevilacqua","sequence":"additional","affiliation":[{"name":"Department of Urology University of Modena and Reggio Emilia  Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Eissa","sequence":"additional","affiliation":[{"name":"Department of Urology University of Modena and Reggio Emilia  Modena Italy"},{"name":"Urology Department Faculty of Medicine Tanta University Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Elsherbiny","sequence":"additional","affiliation":[{"name":"Department of Urology University of Modena and Reggio Emilia  Modena Italy"},{"name":"Urology Department Faculty of Medicine Tanta University Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7211-0485","authenticated-orcid":false,"given":"Maria C.","family":"Sighinolfi","sequence":"additional","affiliation":[{"name":"Department of Urology University of Modena and Reggio Emilia  Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna","family":"Chester","sequence":"additional","affiliation":[{"name":"Department of Surgical, Medical, Dental and Morphological Sciences with Interest Transplant, Oncological and Regenerative Medicine University of Modena and Reggio Emilia Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaniko","family":"Kaleci","sequence":"additional","affiliation":[{"name":"Department of Surgical, Medical, Dental and Morphological Sciences with Interest Transplant, Oncological and Regenerative Medicine University of Modena and Reggio Emilia Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9135-0035","authenticated-orcid":false,"given":"Bernardo","family":"Rocco","sequence":"additional","affiliation":[{"name":"Department of Urology University of Modena and Reggio Emilia  Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvatore","family":"Micali","sequence":"additional","affiliation":[{"name":"Department of Urology University of Modena and Reggio Emilia  Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilaria","family":"Bagni","sequence":"additional","affiliation":[{"name":"Department of Pathology Ospedale Policlinico e Nuovo Ospedale Civile S. Agostino Estense Modena University of Modena and Reggio Emilia Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca Reggiani","family":"Bonetti","sequence":"additional","affiliation":[{"name":"Department of Pathology Ospedale Policlinico e Nuovo Ospedale Civile S. Agostino Estense Modena University of Modena and Reggio Emilia Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonino","family":"Maiorana","sequence":"additional","affiliation":[{"name":"Department of Pathology Ospedale Policlinico e Nuovo Ospedale Civile S. Agostino Estense Modena University of Modena and Reggio Emilia Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josep","family":"Malvehy","sequence":"additional","affiliation":[{"name":"Melanoma Unit Dermatology Department Hospital Clinic Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caterina","family":"Longo","sequence":"additional","affiliation":[{"name":"Azienda Unit Sanitaria Locale \u2013 IRCCS di Reggio Emilia Centro Oncologico ad Alta Tecnologia Diagnostica\u2010 Dermatologia Reggio Emilia Modena Italy"},{"name":"Department of Dermatology University of Modena and Reggio Emilia Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3938-610X","authenticated-orcid":false,"given":"Rodolfo","family":"Montironi","sequence":"additional","affiliation":[{"name":"Department of Pathological Anatomy Polytechnic University of the Marche Region School of Medicine United Hospitals Ancona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giampaolo","family":"Bianchi","sequence":"additional","affiliation":[{"name":"Department of Urology University of Modena and Reggio Emilia  Modena Italy"},{"name":"Department of Surgical, Medical, Dental and Morphological Sciences with Interest Transplant, Oncological and Regenerative Medicine University of Modena and Reggio Emilia Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Pellacani","sequence":"additional","affiliation":[{"name":"Department of Surgical, Medical, Dental and Morphological Sciences with Interest Transplant, Oncological and Regenerative Medicine University of Modena and Reggio Emilia Modena Italy"},{"name":"Department of Dermatology University of Modena and Reggio Emilia Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,4,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jso.24109"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2004.09.002"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000049751"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2012.08.025"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2016.08.003"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep27419"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2490-14-86"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2012.04.057"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2010.09591.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/00125480-200101000-00001"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.14984"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.14463"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2013.158"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.13754"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-016-1936-5"},{"key":"e_1_2_8_17_1","unstructured":"MAVIG GmbH.MAVIG VivaScope \u2010 Technical data [Internet]. 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