{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T19:05:34Z","timestamp":1789585534154,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,6,27]],"date-time":"2019-06-27T00:00:00Z","timestamp":1561593600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      To evaluate: (i) the neuro\u2010regenerative potential of\n                      <jats:styled-content style=\"fixed-case\">chitosan membrane (CS<\/jats:styled-content>\n                      \u2010Me) on acutely axotomised autonomic neurones\n                      <jats:italic>in vitro<\/jats:italic>\n                      ; (ii) to exclude the possibility that a pro\u2010regenerative biomaterial could interfere with the proliferation activity of prostate cancer cell lines; (iii) to provide an\n                      <jats:italic>in vivo<\/jats:italic>\n                      proof of the biocompatibility and regeneration promoting effect of\n                      <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content>\n                      \u2010Me in a standardised rat model of peripheral nerve injury and repair; (iv) finally, to evaluate the tissue reaction induced by the degrading material; as previous studies have shown promising effects of\n                      <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content>\n                      \u2010Me for protection of the neurovascular bundles for potency recovery in patients that undergo nerve\u2010sparing radical prostatectomy (RP).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Addressing aim (i), the neuro\u2010regenerative potential, organotypic cultures derived from primary sympathetic ganglia were cultured on\n                      <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content>\n                      \u2010Me over 3\u00a0days and neurite extension and axonal sprouting were evaluated.\n                    <\/jats:p>\n                    <jats:p>\n                      Addressing aim (ii), effects of\n                      <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content>\n                      on cancer cells, different human prostate cancer cell lines (\n                      <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>\n                      3,\n                      <jats:styled-content style=\"fixed-case\">DU<\/jats:styled-content>\n                      \u2010145,\n                      <jats:styled-content style=\"fixed-case\">LN<\/jats:styled-content>\n                      \u2010Cap) were seeded on\n                      <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content>\n                      \u2010coated plates or cultured in the presence of\n                      <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content>\n                      \u2010Me dissolution products.\n                    <\/jats:p>\n                    <jats:p>\n                      Addressing aims (iii) and (iv), functional recovery of peripheral nerve fibres and tissue reaction with the biomaterial,\n                      <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content>\n                      \u2010Me and CS nerve guides were used to repair a median nerve injury in the rat. Functional recovery was evaluated during the post\u2010recovery time by the behavioural grasping test.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content>\n                      \u2010Me significantly stimulated axon elongation from autonomic ganglia in comparison to control conditions in organotypic three\u2010dimensional cultures.\n                      <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content>\n                      coating, as well as the dissolution products of\n                      <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content>\n                      \u2010Me, led to a significantly lower proliferation rate of prostate cancer cell lines\n                      <jats:italic>in vitro<\/jats:italic>\n                      .\n                    <\/jats:p>\n                    <jats:p>\n                      Tissue reaction towards\n                      <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content>\n                      \u2010Me and standard CS nerve guides was similar in the rat median nerve model, as was the outcome of nerve fibre regeneration and functional recovery.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The results of this study provide the first experimental evidence in support of the clinical safety of\n                      <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content>\n                      \u2010Me and of their postulated effectiveness for improving functional recovery after RP. The presented results are coherent in demonstrating that acutely axotomised autonomic neurones show increased neurite outgrowth on\n                      <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content>\n                      \u2010Me substrate, whilst the same substrate reduces prostate cancer cell line proliferation\n                      <jats:italic>in vitro<\/jats:italic>\n                      . Furthermore,\n                      <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content>\n                      \u2010Me do not demonstrate any disadvantage for peripheral nerve repair in a standard animal model.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bju.14834","type":"journal-article","created":{"date-parts":[[2019,5,28]],"date-time":"2019-05-28T04:29:55Z","timestamp":1559017795000},"page":"1063-1076","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["New basic insights on the potential of a chitosan\u2010based medical device for improving functional recovery after radical prostatectomy"],"prefix":"10.1111","volume":"124","author":[{"given":"Luisa","family":"Muratori","sequence":"first","affiliation":[{"name":"Department of Clinical and Biological Sciences Cavalieri Ottolenghi Neuroscience Institute University of Turin Orbassano Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Fregnan","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences Cavalieri Ottolenghi Neuroscience Institute University of Turin Orbassano Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Ronchi","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences Cavalieri Ottolenghi Neuroscience Institute University of Turin Orbassano Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten","family":"Haastert\u2010Talini","sequence":"additional","affiliation":[{"name":"Hannover Medical School Institute of Neuroanatomy and Cell Biology Center for Systems Neuroscience (ZSN) Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Metzen","sequence":"additional","affiliation":[{"name":"Hannover Medical School Institute of Neuroanatomy and Cell Biology Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0260-4601","authenticated-orcid":false,"given":"Riccardo","family":"Bertolo","sequence":"additional","affiliation":[{"name":"Glickman Urological and Kidney Institute Cleveland Clinic Cleveland OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Porpiglia","sequence":"additional","affiliation":[{"name":"Division of Urology Department of Oncology San Luigi Gonzaga Hospital University of Turin Orbassano Italy"},{"name":"Department of Clinical and Biological Sciences San Luigi Gonzaga Hospital University of Turin Orbassano Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6962-831X","authenticated-orcid":false,"given":"Stefano","family":"Geuna","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences Cavalieri Ottolenghi Neuroscience Institute University of Turin Orbassano Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,6,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncponc0288"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/CA.2007.0010"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.euf.2016.11.007"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2015.01.012"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/bju.13959"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748-6041\/11\/4\/045010"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2019.00149"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2013.0621"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejn.13054"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1994.1319"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2014.08.005"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0270(94)00169-H"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0195692"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/1545968312465190"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/514610"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.21117"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0270(03)00098-0"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2009.01.011"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.3171\/jns.2005.103.6.1067"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/brb3.813"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9861(20001120)427:3<333::AID-CNE1>3.0.CO;2-T"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0940-9602(00)80117-X"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-014-0197-4"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2017.04.113"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.08.074"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jemermed.2007.05.043"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.10.040"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/9175248"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.250628"},{"key":"e_1_2_7_31_1","first-page":"5289","article-title":"Nanotechnology versus stem cell engineering: in vitro comparison of neurite inductive potentials","volume":"9","author":"Morano M","year":"2014","journal-title":"Int J Nanomed"},{"key":"e_1_2_7_32_1","first-page":"1321","article-title":"Chitosan derivatives inhibit cell proliferation and induce apoptosis in breast cancer cells","volume":"31","author":"Jiang M","year":"2011","journal-title":"Anticancer Res"},{"key":"e_1_2_7_33_1","first-page":"5136","article-title":"In vitro effects of chitosan nanoparticles on proliferation of human gastric carcinoma cell line MGC803 cells","volume":"11","author":"Qi LF","year":"2005","journal-title":"World J Gastroenterol"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2012.10.009"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2014.08.033"},{"key":"e_1_2_7_36_1","first-page":"4887","article-title":"Human prostate carcinoma cells express enzymatic activity that converts human plasminogen to the angiogenesis inhibitor, angiostatin","volume":"56","author":"Gately S","year":"1996","journal-title":"Cancer Res"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.11110911"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2017.02.026"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/micr.22362"}],"container-title":["BJU International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbju.14834","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bjui-journals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bju.14834","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T10:51:15Z","timestamp":1761735075000},"score":1,"resource":{"primary":{"URL":"https:\/\/bjui-journals.onlinelibrary.wiley.com\/doi\/10.1111\/bju.14834"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6,27]]},"references-count":38,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["10.1111\/bju.14834"],"URL":"https:\/\/doi.org\/10.1111\/bju.14834","archive":["Portico"],"relation":{},"ISSN":["1464-4096","1464-410X"],"issn-type":[{"value":"1464-4096","type":"print"},{"value":"1464-410X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,6,27]]},"assertion":[{"value":"2019-06-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}