{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T13:29:10Z","timestamp":1767706150274,"version":"build-2065373602"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,10,23]],"date-time":"2020-10-23T00:00:00Z","timestamp":1603411200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100009706","name":"Erzincan \u00dcniversitesi","doi-asserted-by":"publisher","award":["# 731377"],"award-info":[{"award-number":["# 731377"]}],"id":[{"id":"10.13039\/501100009706","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To investigate the therapy of stress urinary incontinence in a preclinical setting cells were injected into the urethrae of minipigs; however, cells injected by William\u2019s needle were frequently misplaced or lost; thus, we investigated if needle\u2010free cell injections using a novel waterjet technology facilitates precise injections in the urethral sphincter complex.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Porcine adipose tissue\u2010derived stromal cells (pADSCs) were isolated from boars, expanded, labelled, and injected in the sphincter of female pigs by waterjet employing two different protocols. After incubation for 15\u00a0min or 3\u00a0days, the urethrae of the pigs were examined. Injected cells were visualised by imaging and fluorescence microscopy of tissue sections. DNA of injected male cells was verified by polymerase chain reaction (PCR) of the sex\u2010determining region (SRY) gene. Cell injections by William\u2019s needle served as controls.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The new waterjet technology delivered pADSCs faster and with better on\u2010site precision than the needle injections. Bleeding during or after waterjet injection or other adverse effects, such as swelling or urinary retention, were not observed. Morphologically intact pADSCs were detected in the urethrae of all pigs treated by waterjet. SRY\u2010PCR of chromosomal DNA and detection of recombinant green fluorescent protein verified the injection of viable cells. In contrast, three of four pigs injected by William\u2019s needle displayed no or misplaced cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Transurethral injection of viable pADSCs by waterjet is a simple, fast, precise, and yet gentle new technology. This is the first proof\u2010of\u2010principle concept study providing evidence that a waterjet injects intact cells exactly in the tissue targeted in a preclinical\n                      <jats:italic>in vivo<\/jats:italic>\n                      situation. To further explore the clinical potential of the waterjet technology longer follow\u2010up, as well as incontinence models have to be studied.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bju.15249","type":"journal-article","created":{"date-parts":[[2020,12,4]],"date-time":"2020-12-04T12:21:12Z","timestamp":1607084472000},"page":"463-472","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Rapid and precise delivery of cells in the urethral sphincter complex by a novel needle\u2010free waterjet technology"],"prefix":"10.1111","volume":"127","author":[{"given":"Walter","family":"Linzenbold","sequence":"first","affiliation":[{"name":"Erbe Elektromedizin GmbH T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luise","family":"J\u00e4ger","sequence":"additional","affiliation":[{"name":"Erbe Elektromedizin GmbH T\u00fcbingen 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