{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T22:46:55Z","timestamp":1785192415541,"version":"3.55.0"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,7,5]],"date-time":"2021-07-05T00:00:00Z","timestamp":1625443200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002879","name":"Canadian HIV Trials Network, Canadian Institutes of Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002879","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To undertake the first comprehensive evaluation of the urinary microbiota associated with Hunner lesion (HL) interstitial cystitis\/bladder pain syndrome (IC\/BPS). Despite no previous identification of a distinct IC\/BPS microbial urotype, HL IC\/BPS, an inflammatory subtype of IC\/BPS, was hypothesized most likely to be associated with a specific bacterial species or microbial pattern.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Participants and Methods<\/jats:title>\n                    <jats:p>The bacterial microbiota of midstream urine specimens from HL IC\/BPS and age\u2010 and gender\u2010matched IC\/BPS patients without HL (non\u2010HL IC\/BPS) were examined using the pan\u2010bacterial domain clinical\u2010level molecular diagnostic Pacific Biosciences full\u2010length 16S gene sequencing protocol, informatics pipeline and database. We characterized the differential presence, abundances, and diversity of species, as well as gender\u2010specific differences between and among HL and non\u2010HL IC\/BPS patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 59 patients with IC\/BPS were enrolled (29 HL, 30 non\u2010HL; 43 women, 16 men) from a single centre and the microbiota in midstream urine specimens was available for comparison. The species abundance differentiation between the HL and non\u2010HL groups (12 species) was not significantly different after Bonferroni adjustments for multiple comparisons. Similarly, the nine differentiating species noted between female HL and non\u2010HL patients were not significantly different after similar statistical correction. However, four species abundances (out of the 10 species differences identified prior to correction) remained significantly different between male HL and non\u2010HL subjects:\n                      <jats:italic>Negativicoccus succinivorans, Porphyromonas somerae, Mobiluncus curtisii<\/jats:italic>\n                      and\n                      <jats:italic>Corynebacterium<\/jats:italic>\n                      <jats:italic>renale<\/jats:italic>\n                      . Shannon diversity metrics showed significantly higher diversity among HL male patients than HL female patients (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.045), but no significant diversity differences between HL and non\u2010HL patients overall.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We were not able to identify a unique pathogenic urinary microbiota that differentiates all HL from all non\u2010HL IC\/BPS. It is likely that the male\u2010specific differences resulted from colonization\/contamination remote from the bladder. We were not able to show that bacteria play an important role in patients with HL IC\/BPS.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bju.15519","type":"journal-article","created":{"date-parts":[[2021,6,18]],"date-time":"2021-06-18T10:08:15Z","timestamp":1624010895000},"page":"104-112","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["The bacterial microbiota of Hunner lesion interstitial cystitis\/bladder pain syndrome"],"prefix":"10.1111","volume":"129","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4865-7293","authenticated-orcid":false,"given":"J. Curtis","family":"Nickel","sequence":"first","affiliation":[{"name":"Department of Urology Queen\u2019s University Kingston ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Garth D.","family":"Ehrlich","sequence":"additional","affiliation":[{"name":"Department of MIcrobiology and Immunology Drexel University Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaroslaw E.","family":"Krol","sequence":"additional","affiliation":[{"name":"Department of MIcrobiology and Immunology Drexel University Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azad","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of MIcrobiology and Immunology Drexel University Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhaswati","family":"Sen","sequence":"additional","affiliation":[{"name":"Department of MIcrobiology and Immunology Drexel University Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Archana","family":"Bhat","sequence":"additional","affiliation":[{"name":"Department of MIcrobiology and Immunology Drexel University Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua C.","family":"Mell","sequence":"additional","affiliation":[{"name":"Department of MIcrobiology and Immunology Drexel University Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. Christopher","family":"Doiron","sequence":"additional","affiliation":[{"name":"Department of Urology Queen\u2019s University Kingston ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerri\u2010Lynn","family":"Kelly","sequence":"additional","affiliation":[{"name":"Department of Urology Queen\u2019s University Kingston ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua P.","family":"Earl","sequence":"additional","affiliation":[{"name":"Department of MIcrobiology and Immunology Drexel University Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,7,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2007.09.019"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2016.04.067"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/JU.0000000000001031"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00675-18"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-12-205"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm8030415"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0000000000001892"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00092"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-019-03942-9"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/SPV.0000000000000854"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2015.09.075"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00345-019-02764-0"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/21681805.2020.1744714"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/21681805.2020.1730948"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/iju.12370"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2020.04.067"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2011.03.064"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2011.03.132"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.24500"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(99)80333-1"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-018-0569-2"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-018-1501-6"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq461"},{"key":"e_1_2_7_25_1","unstructured":"JolSJ.Make a Venn Diagram. 2015. 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