{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T16:27:10Z","timestamp":1788798430994,"version":"build-2803163510"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,9,13]],"date-time":"2021-09-13T00:00:00Z","timestamp":1631491200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006469","name":"Fundo para o Desenvolvimento das Ci\u00eancias e da Tecnologia","doi-asserted-by":"publisher","award":["ID: 30136"],"award-info":[{"award-number":["ID: 30136"]}],"id":[{"id":"10.13039\/501100006469","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To investigate the predictive value of different immunological markers on treatment outcomes after bacille Calmette\u2013Gu\u00e9rin (BCG) induction in high\u2010risk non\u2010muscle\u2010invasive bladder cancer (NMIBC).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Patients and Methods<\/jats:title>\n                    <jats:p>Patients who underwent transurethral resection of bladder tumour for NMIBC were assessed for study eligibility. Urine and blood samples were taken from patients at baseline (immediately before first dose of induction) and after induction (4\u2009h after last [sixth] dose). Urine samples were evaluated for interleukin (IL)\u20102 and IL\u201010 by solid\u2010phase enzyme\u2010linked immunosorbent assay. Blood samples were evaluated for tumour necrosis factor \u03b1 (TNF\u2010\u03b1), cytotoxic T\u2010lymphocyte antigen 4 (CTLA\u20104) and transcription factors (TFs) (GATA\u2010binding protein 3 [GATA3], T\u2010box expressed in T cells [T\u2010bet], and forkhead box protein 3 [FoxP3]) using quantitative reverse transcriptase\u2010polymerase chain reaction analysis. Change pattern and fold change of each evaluable marker was assessed in relation to different treatment outcomes (initial complete response [ICR]\/recurrence\/progression).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Between July 2013 and May 2019, 204 patients were included. Among evaluable markers, urinary IL\u20102 and serum TNF\u2010\u03b1 increased in all patients, serum CTLA\u20104 and FoxP3\n                      <jats:sup>+<\/jats:sup>\n                      showed a predominant decreased pattern in 188 (92.2%) and 192 (94.1%) patients, respectively. An ICR was achieved in 186 (91.2%) patients. Serum TNF\u2010\u03b1 fold change and urinary IL\u201010 change pattern were significantly associated with an ICR (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.001 and\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.03, respectively). At a median (range) follow\u2010up of 37\u2009(20\u201388)\u2009months, 104 (56%) patients developed recurrence. Urinary IL\u201010, serum CTLA\u20104, T\u2010bet\n                      <jats:sup>+<\/jats:sup>\n                      , FoxP3\n                      <jats:sup>+<\/jats:sup>\n                      change patterns and GATA3\n                      <jats:sup>+<\/jats:sup>\n                      \/T\u2010bet\n                      <jats:sup>+<\/jats:sup>\n                      ratio were significantly associated with tumour recurrence (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.001,\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.001,\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.02,\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.009 and\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.001, respectively). Tumour progression occurred in 34 (18.3%) patients. Urinary IL\u201010, serum CTLA\u20104, serum T\u2010bet\n                      <jats:sup>+<\/jats:sup>\n                      change patterns and GATA3\n                      <jats:sup>+<\/jats:sup>\n                      \/T\u2010bet\n                      <jats:sup>+<\/jats:sup>\n                      ratio were independent predictors of tumour progression (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.001,\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.001,\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.02 and\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.001, respectively).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Urinary IL\u201010 and serum TNF\u2010\u03b1 can significantly predict ICR. Moreover, change pattern of urinary IL\u201010, serum CTLA\u20104, TFs (GATA3, T\u2010bet and FoxP3) and GATA3\n                      <jats:sup>+<\/jats:sup>\n                      \/T\u2010bet\n                      <jats:sup>+<\/jats:sup>\n                      ratio after BCG induction can independently predict further BCG response. These markers could be implemented in clinical practice when management options are discussed or in systems with severe BCG shortage.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bju.15582","type":"journal-article","created":{"date-parts":[[2021,8,27]],"date-time":"2021-08-27T04:09:50Z","timestamp":1630037390000},"page":"444-453","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Predictive value of immunological markers after bacille Calmette\u2013Gu\u00e9rin induction in bladder cancer"],"prefix":"10.1111","volume":"130","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7077-8094","authenticated-orcid":false,"given":"Amr A.","family":"Elsawy","sequence":"first","affiliation":[{"name":"Urology and Nephrology Center Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hassan","family":"Abol\u2010Enein","sequence":"additional","affiliation":[{"name":"Urology and Nephrology Center Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud","family":"Laymon","sequence":"additional","affiliation":[{"name":"Urology and Nephrology Center Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asmaa E.","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Urology and Nephrology Center Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Essam","sequence":"additional","affiliation":[{"name":"Urology and Nephrology Center Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman T.","family":"Hamam","sequence":"additional","affiliation":[{"name":"Urology and Nephrology Center Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdel\u2010Aziz A.","family":"Zidan","sequence":"additional","affiliation":[{"name":"Department of Zoology Faculty of Science Damanhour University  Damanhour Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed H.","family":"Zahran","sequence":"additional","affiliation":[{"name":"Urology and Nephrology Center Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2699-2629","authenticated-orcid":false,"given":"Ahmed A.","family":"Shokeir","sequence":"additional","affiliation":[{"name":"Urology and Nephrology Center Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amira","family":"Awadalla","sequence":"additional","affiliation":[{"name":"Urology and Nephrology Center Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,9,13]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2015.06.045"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2011.05.045"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11934-019-0952-y"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2007.10.006"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2009.02.053"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1995.tb05560.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-003-0384-9"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.11352"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2015.06.023"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2011.10.144"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)63839-4"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urolonc.2020.07.013"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urolonc.2020.06.012"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000059681.67567.12"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-016-1945-z"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urolonc.2013.06.008"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00345-018-2332-5"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41585-018-0055-4"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-019-02443-4"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.9537"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2623"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-016-0891-z"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1103336"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2019.1602460"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2015.4064"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2010.10020.x"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0204745"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2017.1358330"}],"container-title":["BJU International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bju.15582","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/bju.15582","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bjui-journals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bju.15582","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T07:58:45Z","timestamp":1761724725000},"score":1,"resource":{"primary":{"URL":"https:\/\/bjui-journals.onlinelibrary.wiley.com\/doi\/10.1111\/bju.15582"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,13]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,10]]}},"alternative-id":["10.1111\/bju.15582"],"URL":"https:\/\/doi.org\/10.1111\/bju.15582","archive":["Portico"],"relation":{},"ISSN":["1464-4096","1464-410X"],"issn-type":[{"value":"1464-4096","type":"print"},{"value":"1464-410X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,13]]},"assertion":[{"value":"2021-05-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}