{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T11:37:40Z","timestamp":1793446660373,"version":"4.5.0"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,12,11]],"date-time":"2023-12-11T00:00:00Z","timestamp":1702252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009539","name":"Life Sciences Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009539","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22K11364"],"award-info":[{"award-number":["22K11364"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To evaluate the effect of intravenous administration of human multilineage\u2010differentiating stress\u2010enduring (Muse) cells on rat postoperative erectile dysfunction (ED) with cavernous nerve (CN) injury without an immunosuppressant.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Male Sprague\u2013Dawley rats were randomised into three groups after CN crush injury. Either human\u2010Muse cells, non\u2010Muse mesenchymal stem cells (MSCs) (both 1.0\u2009\u00d7\u200910\n                      <jats:sup>5<\/jats:sup>\n                      cells), or vehicle was infused intravenously at 3\u2009h after CN injury without immunosuppressant. Erectile function was assessed by measuring intracavernous pressure (ICP) and arterial pressure (AP) during pelvic nerve electrostimulation 28\u2009days after surgery. At 48\u2009h and 28\u2009days after intravenous infusion of Muse cells, the homing of Muse cells and non\u2010Muse MSCs was evaluated in the major pelvic ganglion (MPG) after CN injury. In addition, expressions of C\u2010X\u2010C motif chemokine ligand (\n                      <jats:italic>Cxcl12<\/jats:italic>\n                      ) and glial cell line\u2010derived neurotrophic factor (\n                      <jats:italic>Gdnf<\/jats:italic>\n                      ) in the MPG were examined by real\u2010time polymerase chain reaction. Statistical analyses and comparisons among groups were performed using one\u2010way analysis of variance followed by the Tukey test for parametric data and Kruskal\u2013Wallis test followed by the Dunn\u2013Bonferroni test for non\u2010parametric data.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The mean (SEM) ICP\/AP values at 28\u2009days were 0.51\u2009(0.02) in the Muse cell group, 0.37\u2009(0.03) in the non\u2010Muse MSC group, and 0.36 (0.04) in the vehicle group, showing a significant positive response in the Muse cell group compared with the non\u2010Muse and vehicle groups (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.013 and\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.010, respectively). In the MPG, Muse cells were observed to be engrafted at 48\u2009h and expressed Schwann cell markers S100 (~46%) and glial fibrillary acidic protein (~24%) at 28\u2009days, while non\u2010Muse MSCs were basically not engrafted at 48\u2009h. Higher gene expression of\n                      <jats:italic>Cxcl12<\/jats:italic>\n                      (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.048) and\n                      <jats:italic>Gdnf<\/jats:italic>\n                      (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.040) was found in the MPG of the Muse group than in the vehicle group 48\u2009h after infusion.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Intravenously engrafted human Muse cells recovered rat erectile function after CN injury in a rat model possibly by upregulating\n                      <jats:italic>Cxcl12<\/jats:italic>\n                      and\n                      <jats:italic>Gdnf<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bju.16232","type":"journal-article","created":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T04:34:54Z","timestamp":1700541294000},"page":"332-340","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Intravenously engrafted human multilineage\u2010differentiating stress\u2010enduring (Muse) cells rescue erectile function after rat cavernous nerve injury"],"prefix":"10.1111","volume":"133","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3278-7816","authenticated-orcid":false,"given":"Juntaro","family":"Koyama","sequence":"first","affiliation":[{"name":"Department of Urology Tohoku University Graduate School of Medicine  Sendai Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinichi","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Department of Urology Tohoku University Graduate School of Medicine  Sendai 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